[Cardioprotective effect of N-stearoylethanolamine under the anaphylactic shock in guinea pigs].

It was shown for the first time that endogenous cannabimimetic compound N-stearoylethanolamine (NSE) administered at the dose 65 mg/kg of body weight during two weeks before anaphylaxis had cardioprotective properties on the model of anaphylactic shock in the guinea pigs. NSE reliably normalized the content of histamine and nitric oxide, decreased the activity both inducible and constitutive NO-synthases, prevented accumulation of thiobarbituric acid reactive substances (TBARS) and normalized the activity of catalase, superoxide dismutase and glutathione peroxidase in cardiomyocytes of animals under anaphylaxis. About of 70% of the NSE-treated animals survived under the anaphylactic shock.

[Changes of nitric oxide level at different stages of primary open-angle glaucoma].

The contents of NO stable metabolites in tears, and blood serum of the patients with different stages of open-angle glaucoma have been studied. The increasing of NO stable metabolite contents was found in tears, aqueous humor and blood serum during glaucoma progression. The depletion of glaucoma pathogenesis oxidative link was found at the terminal stage of the disease.

[Relationship between polyreactive properties of immunoglobulins and level of lipids in them].

Presented paper deals with the relationship between immunoglobulin polyreactive properties and its lipid composition. Serum blood immunoglobulin fraction of an intact rabbit as an experimental model was used. Immunoglobulins (Ig) obtained by this way were transformed into polyreactive immunoglobulins (PRIg) by treatment with chaotropic agent KSCN or reactive oxygen species (ROS) with usage of Fe2+, EDTA and ascorbic acid.

[Characteristics of the effect of implantation of the human ApoA1 gene on the condition of hepatocytes in rats of varying age].

The results obtained show the essential changes in functional state of hepatocyte's plasmatic membrane due to the implantation of human ApoA1 gene to the rat liver. The changes in phospholipid composition, hyperpolarization, increase in activity of membrane bound enzymes, cytochrome P-450 and biosynthesis of liver total proteins have been found. The essential changes characterizing cell effect were more marked in the adult rats, and membrane effect in the old ones.

[Effect of n-palmitoylethanolamine on energy-dependent transport of Ca2+ in intracellular structures of myometrium and their phospholipid composition].

N-palmitoylethanolamine (NPE) was studied for its effect on the systems of energy-dependent transport of Ca2+ in the intracell structures (mitochondria, endoplasmic reticulum) of permeabilized cells of smooth muscles of the rat uterus as well as on the lipid composition of myocytes. NPE in concentration of 10(-5) M partially (by 30-50%) inhibited energy-dependent accumulation of Ca2+ in endoplasmic reticulum and mitochondria of myometrium cells permeabilized by means of treatment of myocytes suspension by digitonin (0.1 mg/ml).

[Effect of N-palmitoylethanolamine on energy-dependent transport of Ca2+ in vesicles of myometrium sarcolemma and their phospholipid composition].

N-palmitoylethanolamine (NPE) was studied for their effect on calcium pump of pig myometrium sarcolemma. NPE in concentration of 10 microM, stimulated by 28-46% Mg2+, ATP-dependent accumulation of Ca2+ in vesicles of plasmatic membrane of uterus myocytes taking absolutely no effect on passive release of this cation from them. NPE modified phospholipid composition of sarcolemma, causing the increase of percentage content of phosphatidylinositol (by 20.

[Effect of N-palmitoyl- and N-stearoylethanolamines on lipid peroxidation in mouse tissues in metastatic Lewis carcinoma].

The ability of two-cell minor lipid components: N-palmitoyl- and N-stearoylethanolamines to influence the Lewis lung carcinoma metastases has been studied. It is shown that these compounds decrease the quantity and the volume of metastases. Acyl derivatives of ethanolamine inhibit simultaneously the process of lipid peroxidation.

[The characteristics of lectin binding to the surface of differentiated neuroblastoma C 1300 cells].

Peculiarities of the binding of lectins of castor bean, cochlea, soya bean, lime bean and wheat germs to the surface of differential neuroblastoma C 1300 N 18 cells have been studied using the method electron cytochemistry. It is found that the quantity of the bound lectin conjugates with colloidal gold on the surface of differentiated cells varied considerably from that on the surface of nondifferentiated cells.

[Morphological differentiation and change in the lipid composition of neuroblastoma C1300 cells treated with gangliosides].

The effect of exogenous gangliosides on the morphological differentiation of neuroblastoma 1300 N18 cells was studied Simultaneously the content of gangliosides and lipid composition of the cells was investigated. Gangliosides were shown to increase the quantity of cells with long neurites. This effect depended on the dose of gangliosides.

[Detection of N-acylethanolamine phospholipids in neuroblastoma C1300 cells].

Phospholipids with high chromatographic mobility are detected in cells of murine neuroblastoma C 1300. One of them is identified (proceeding from data of successive chemical degradation) as N-acylphosphatidylethanolamine. It may be a precursor of physiologically active N-acylethanolamines.

[Identification and properties of insulin receptors in neuroblastoma C 1300 N 18 cells in various functional states].

The presence of insulin receptors in neuroblastoma C 1300 N18 cells was shown by the method of the [125I]insulin binding with cells as well as by the electron-cytochemical methods based on the analysis of the binding of colloid gold-labelled insulin and of agglutinin of wheat embryos with the surface of plasma membrane. It is established that the number and distribution of insulin receptors depend on the functional state of cells. Expression of receptors on the membrane increases after 5'-deoxyuridine-induced differentiation of cells.

[Localization and functional role of gangliosides from nerve tissue of vertebrates].

Gangliosides in the animal organism are typical components of plasma membranes of nerve cells in which the concentration of polysialogangliosides is especially high. The high concentration of tri- and tetrasialoganyliosides and ganglioside GD1b is peculiar to primary cultures of nerve cells, whereas these gangliosides are practically not present in the culture of the transformed nerve cells which have lost their ability to sinaptogenesis, they are not found in cultures of oligodendro- and astroglia as well. The addition of exogenic gangliosides to nerve cells cultures stimulates the formation of processes in these cells and promotes their survivability.

[Transmembrane lipid transport between lecithin liposomes and neuroblastoma C1300 N18 cells].

The method of double isotopic labels was used to study dynamics of lipid metabolism between neuroblastoma C 1300 N 18 A 1 cells and lecithin liposomes which contained 4.5-5 mumol of lecithin in 1 ml of the suspension. The cell lipids were labelled by radioactive carbon and cultivated on the medium with [1-14C] sodium acetate, phosphatidylcholine of liposomes was labelled by tritium.

[Inorganic pyrophosphatase activity of the mouse spleen in the immune response and after treatment with bis-phosphonates].

The inorganic pyrophosphatase activity was determined in different tissues of mice. The immunization of mice by sheep erythrocytes increased the inorganic pyrophosphatase activity of the spleen. The in vivo administration of bisphosphonates (40 mg per 1 g of mass), which are structural analogs of inorganic pyrophosphate (methylene bisphosphonic acid--MBPA, hydroxyethylidene bisphosphonic acid--HEBPA and aminomethylene bisphosphonic acid--AMBPA), inhibited the inorganic pyrophosphatase activity only by MBPA in the thymus and spleen but not in liver.

[Modification of the lipid composition of neuroblastoma C1300 cells using liposomes].

An essential increase in the total cholesterol content with a significant growth in the level of its esters was observed in neuroblastoma C1300 cells differentiated by means of 5'-bromodesoxyuridine and incubated for 1 h with lecithin-cholesterol liposomes (1:1 mol/mol). These cells also displayed an increased amount of polyunsaturated fatty acids in the composition of lecithin molecules and the appearance of lysolicethin as well. Incubation of the cells with lecithin liposomes was accompanied by a decrease in the content of cholincholesterol in the cells.

[Relation between the functional characteristics of fast sodium channels and lipid composition of neuroblastoma C1300 cells].

The paper deals with functional characteristics of fast sodium channels modified by liposomes of differentiated neuroblastoma cells. Cells with a 60% increased total cholesterol content were used. The cells were found to contain lysolecithin.

[Cultured neuroblastoma cells in biochemical studies].

Neuroblastoma is a spontaneous tumour of animals and people. Tumour strains maintained as transplanted tumours and constant cell lines in suspension and monolayer cultures in vitro are obtained. The neuroblastoma cells are able to spontaneous and induced differentiation.

[Various physico-chemical properties of inorganic pyrophosphatase from the mouse spleen].

Manganese, calcium and mercury ions, as well as p-chloromercury benzoate and dithiothreitol are studied for their effect on the activity of inorganic pyrophosphatase (EC 3.6.1.

[Changes in the lipid composition of murine neuroblastoma C 1300 cells during the process of differentiation].

The paper deals with the lipid composition of nondifferentiated cells at the logarithmic and stationary growth phases as well as with differentiated cells of C 1300 neuroblastoma adopted to the Eagle medium. It is shown that phospholipids and cholesterol dominate at the studied growth phases among lipids. Their amount in the differentiation process is thrice as high calculating per cells.

[Transport of Rb+ via activated sodium channels of clone N 18 phi 1 neuroblastoma cells].

A study was made of the Rb+ transport via activated sodium channels of clone N 18 phi 1 neuroblastoma cells cultured in the Eagle medium with 10% bovine serum. The time of population doubling was about 10 h. The cell differentiation was induced by adding bromdeoxyuridine in a concentration of 1-4 10(-5) M.

[Effect of inorganic pyrophosphate and its diphosphonate analogs on glucose-6-phosphatase dehydrogenase activity of mouse organs].

The glucose-6-phosphatase dehydrogenase (EC 1.1.1.

[Effect of methylene diphosphonic acid on certain processes in animal tissues].

It was shown that methylene diphosphonic acid, a structural analogue of pyrophosphate, exhibiting immunodepressant properties, in a dose of 40 micrograms/g body weight inhibits by 20% the activity of inorganic pyrophosphatase in the spleen of BALB mice and does not affect this enzyme in the liver or thymus. In noninbred albino mice inhibition of the activity of the enzyme was recorded only as a result of administering the preparation in a dose of 80 micrograms/g. Histological data on the kidneys, liver, lungs, spleen, lymphatic nodes and thymus indicate that the preparation does not exert a significant toxic action on the body.

[Inhibition of mouse spleen inorganic pyrophosphatase by methylene diphosphonic acid].

Some properties of mouse spleen cytosol inorgainc pyrophosphatase (PPi-ase) (E. C. 3.