Design, synthesis, and biological evaluation of novel pleuromutilin derivatives with methicillin-resistant Staphylococcus aureus -targeting phenol linker groups.

Methicillin-resistant Staphylococcus aureus (MRSA) remains a significant global health threat, necessitating the development of new therapeutic agents. Pleuromutilin derivatives offer a promising solution due to their potent antibacterial activity, particularly against Gram-positive bacteria such as MRSA. In this study, we synthesized a series of pleuromutilin derivatives with phenol linker containing C14 side chains and evaluated in vitro and in vivo antibacterial activities.

Extracerebral Normalization of F-FDG PET Imaging Combined with Behavioral CRS-R Scores Predict Recovery from Disorders of Consciousness.

Background: Identifying patients likely to regain consciousness early on is a challenge. The assessment of consciousness levels and the prediction of wakefulness probabilities are facilitated by F-fluorodeoxyglucose (F-FDG) positron emission tomography (PET). This study aimed to develop a prognostic model for predicting 1-year postinjury outcomes in prolonged disorders of consciousness (DoC) using F-FDG PET alongside clinical behavioral scores.

Prospective Study Comparing [Tc]Tc-DP-FAPI Quantitative SPECT/CT with [Ga]Ga-FAPI-04 PET/CT in Patients with Gastrointestinal Tumors.

Over the past decade, [Ga]Ga-FAPI-04 positron emission tomography (PET)/CT imaging has been widely used for the treatment of various tumors. However, the application of TC-labeled fibroblast activation protein inhibitors in tumors has been less studied. Our team previously demonstrated the safe biological distribution of [Tc]Tc-DP-FAPI in the human body.

Decomposed FDG PET-based phenotypic heterogeneity predicting clinical prognosis and decision-making in temporal lobe epilepsy patients.

Objective: This study utilized a data-driven Bayesian model to automatically identify distinct latent disease factors represented by overlapping glucose metabolism patterns from F-Fluorodeoxyglucose PET (F-FDG PET) to analyze heterogeneity among patients with TLE.

Methods: We employed unsupervised machine learning to estimate latent disease factors from F-FDG PET scans, representing whole-brain glucose metabolism patterns in seventy patients with TLE. We estimated the extent to which multiple distinct factors were expressed within each participant and analyzed their relevance to epilepsy burden, including seizure onset, duration, and frequency.

Design and Synthesis of Pleuromutilin Derivatives as Antibacterial Agents Using Quantitative Structure-Activity Relationship Model.

The quantitative structure-activity relationship (QSAR) is one of the most popular methods for the virtual screening of new drug leads and optimization. Herein, we collected a dataset of 955 MIC values of pleuromutilin derivatives to construct a 2D-QSAR model with an accuracy of 80% and a 3D-QSAR model with a non-cross-validated correlation coefficient () of 0.9836 and a cross-validated correlation coefficient () of 0.

Performance Evaluation of the uMI Panorama PET/CT System in Accordance with the National Electrical Manufacturers Association NU 2-2018 Standard.

The uMI Panorama is a novel PET/CT system using silicon photomultiplier and application-specific integrated circuit technologies and providing exceptional spatial and time-of-flight (TOF) resolutions. The objective of this study was to assess the physical performance of the uMI Panorama in accordance with the National Electrical Manufacturers Association (NEMA) NU 2-2018 standard. Spatial resolution, sensitivity, count rate performance, accuracy, image quality, and TOF resolution were evaluated in accordance with the guidelines outlined in the NEMA NU 2-2018 standard.

Surgical evidence-based comparison of [Ga]Ga-FAPI-04 PET and MRI-DWI for assisting debulking surgery in ovarian cancer patients.

Purpose: Imaging assessment of abdominopelvic tumor burden is crucial for debulking surgery decision in ovarian cancer patients. This study aims to compare the efficiency of [Ga]Ga-FAPI-04 FAPI PET and MRI-DWI in the preoperative evaluation and its potential impact to debulking surgery decision.

Methods: Thirty-six patients with suspected/confirmed ovarian cancer were enrolled and underwent integrated [Ga]Ga-FAPI-04 PET/MRI.

Head-to-Head Comparison of Ga-PSMA-11 with Ga-P137 in Patients with Suspected Prostate Cancer.

P137 is a novel oxalyldiaminopropionic acid-urea-based prostate-specific membrane antigen (PSMA) targeting agent. This study compared the uptake patterns of Ga-P137 and the FDA-approved PET tracer Ga-PSMA-11 for diagnosing prostate cancer (PCa). Sixteen patients suspected of PCa were scanned by Ga-PSMA-11 and Ga-P137 PET/CT, respectively, followed by prospective analysis.

First-in-Humans PET Imaging of Mutation Status in Non-Small Cell Lung and Colorectal Cancer Patients Using [F]PFPMD.

Kirsten rat sarcoma () mutations are an important marker for tumor-targeted therapy. In this study, we sought to develop a KRAS oncoprotein-targeted PET tracer and to evaluate its translational potential for noninvasive imaging of the mutation in non-small cell lung cancer (NSCLC) and colorectal cancer (CRC) patients. [F]PFPMD was synthesized on the basis of AMG510 (sotorasib) by attaching a polyethylene glycol chain to the quinazolinone structure.

The role of the symbiotic microecosystem in cancer: gut microbiota, metabolome, and host immunome.

The gut microbiota is not just a simple nutritional symbiosis that parasitizes the host; it is a complex and dynamic ecosystem that coevolves actively with the host and is involved in a variety of biological activities such as circadian rhythm regulation, energy metabolism, and immune response. The development of the immune system and immunological functions are significantly influenced by the interaction between the host and the microbiota. The interactions between gut microbiota and cancer are of a complex nature.

Ga-FAPI-04 PET for Surveillance of Anastomotic Recurrence in Postoperative Patients with Gastrointestinal Cancer: a Comparative Study with F-FDG PET.

Purpose: This study aims to compare the diagnostic efficacy of Ga-FAPI-04 PET and F-FDG PET for detecting anastomotic recurrence in postoperative patients with gastrointestinal cancer, and to characterize the signal pattern over time at surgical wounds on both PET imaging.

Methods: Gastrointestinal cancer patients who planned to Ga-FAPI-04 and F-FDG PET/CT imaging for postoperative surveillance were involved. The SUV at surgical wounds were assessed.

Novel pyridinium cationic pleuromutilin analogues overcoming bacterial multidrug resistance.

A series of pyridinium cation-substituted pleuromutilin analogues were designed, synthesized and evaluated for their antibacterial activities in vitro and in vivo. Most derivatives showed potent antibacterial activities, especially e4 that displayed the highest antibacterial activity against multi-drug resistant bacteria and was subjected to time-kill kinetics, resistance studies, cytotoxicity and molecular docking assays. Molecular docking results, scanning electron microscopy and o-nitrophenyl-β-galactopyranoside tests showed that e4 not only inhibited bacterial protein synthesis but also disrupted bacterial cell walls.

Pharmacokinetics of tenvermectin in swine, a novel antiparasitic drug candidate-comparison with ivermectin.

Tenvermectin (TVM) is a novel 16-membered macrolide compound isolated and purified from the fermentation broth of genetically engineered Streptomyces avermitilis strain MHJ1011. TVM and ivermectin were administered at the dose of 0.3 mg/kg body weight through a single subcutaneous injection route followed by plasma collectiom and analysis at different time intervals.

Mixture-modelling-based Bayesian MH-RM algorithm for the multidimensional 4PLM.

Several recent works have tackled the estimation issue for the unidimensional four-parameter logistic model (4PLM). Despite these efforts, the issue remains a challenge for the multidimensional 4PLM (M4PLM). Fu et al.

Safety, Biodistribution, and Dosimetry Study of Meplazumab, a Potential COVID-19 Therapeutic Drug, with I-Labeling and SPECT Imaging.

Coronavirus disease 2019 (COVID-19) is a serious threat to public health and is in urgent need of specific drugs. Meplazumab, a humanized monoclonal antibody targeting CD147, was confirmed to competitively block the binding between the spike of syndrome coronavirus 2 (SARS-CoV-2) and CD147, making meplazumab a promising candidate drug for COVID-19. In this study, biodistribution and dosimetry of I-labeled meplazumab were performed to further evaluate its potential as a therapeutic drug for COVID-19.

Static and Dynamic Plantar Pressure Distribution in 94 Patients with Different Stages of Unilateral Knee Osteoarthritis Using the Footscan® Platform System: An Observational Study.

BACKGROUND Plantar pressure analysis is widely used in the study of knee osteoarthritis (KOA). The present study aimed to investigate the static and dynamic plantar pressure distribution in patients with different stages of unilateral KOA using the Footscan® platform system. MATERIAL AND METHODS We recruited 94 patients aged 61.

Bibliometric analysis of human monkeypox research from 1975 to 2022 and novel prevention and control strategies.

Background: Since human monkeypox was reported, many related literatures have been published. This study aimed to evaluate the research hotspots and future development trends of human monkeypox by a bibliometric analysis, to analyze the preventive and control measures of various countries in response to human monkeypox outbreaks.

Methods: The Web of Science Core Collection database was searched for all monkeypox related literature published from 1975 to 2022, and the search strategy was "TS = monkeypox.

Epidemiological Characteristics of COVID-19 and Effective Public Health Interventions in Shenzhen, China.

Objectives: This study aims to analyze and summarize the epidemic characteristics of coronavirus disease 2019 (COVID-19), and the public heath interventions in Shenzhen from 1 January 2022 to 4 April 2022, hoping to provide useful reference for resurgence.

Methods: Data were extracted from the website of Shenzhen Municipal Health Commission from 1 January 2022 to 4 April 2022. The number of new indigenous patients, imported patients, symptomatic and asymptomatic patients, age, gender, regional distribution, screening routes, and clinical subtype were analyzed.

Establishment and Optimization of Radiomics Algorithms for Prediction of KRAS Gene Mutation by Integration of NSCLC Gene Mutation Mutual Exclusion Information.

To assess the significance of mutation mutual exclusion information in the optimization of radiomics algorithms for predicting gene mutation. We retrospectively analyzed 258 non-small cell lung cancer (NSCLC) patients. Patients were randomly divided into training ( = 180) and validation ( = 78) cohorts.

HMGB1/PTEN/PI3K axis participates in the peripheral immune cell differentiation in two representative TCM syndromes of chronic hepatitis B patients.

Liver depression and spleen deficiency syndrome (LDSDS) and spleen-gastric damp-heat syndrome (SGDHS) are two major traditional Chinese medicine syndromes observed in chronic hepatitis B (CHB). Both syndromes exhibit significant differences in the pathogenesis and prognosis, and are closely related to the immune system. However, the underlying mechanisms are largely unknown.

Comparative serum microRNA array analysis of the spleen-stomach dampness-heat syndrome in different diseases: Chronic hepatitis B and chronic gastritis.

Spleen-stomach dampness-heat syndrome (SSDHS) is the common Traditional Chinese Medicine (TCM) syndrome observed in both chronic hepatitis B (CHB) and chronic gastritis (CG). The specialized TCM prescription for CHB and CG patients with SSDHS is same, but there is limited information about the biological characteristics of this TCM syndrome. This study aimed to identify the serum miRNAs profile for the SSDHS in two different diseases in order to evaluate the miRNA-mediated biological characteristics of this TCM syndrome.

Automated Test Assembly for Multistage Testing With Cognitive Diagnosis.

Computer multistage adaptive test (MST) combines the advantages of paper and pencil-based test (P&P) and computer-adaptive test (CAT). As CAT, MST is adaptive based on modules; as P&P, MST can meet the need of test developers to manage test forms and keep test forms parallel. Cognitive diagnosis (CD) can accurately measure students' knowledge states (KSs) and provide diagnostic information, which is conducive to student's self-learning and teacher's targeted teaching.

Pattern of COVID-19 in Sichuan province, China: A descriptive epidemiological analysis.

This study described the epidemiology of 487 confirmed coronavirus disease 2019 (COVID-19) cases in Sichuan province of China, and aimed to provide epidemiological evidence to support public health decision making. Epidemiological information of 487 COVID-19 cases were collected from the official websites of 21 districts (including 18 cities, 3 autonomous prefecture) health commissions within Sichuan between 21st of January 2020 to 17th of April 2020. We focus on the single-day diagnosis, demographics (gender and age), regional distribution, incubation period and symptoms.