State-of-the-Art Light-Driven Hydrogen Generation from Formic Acid and Utilization in Enzymatic Hydrogenations.

A concept of combining photocatalytically generated hydrogen with green enzymatic reductions is demonstrated. The developed photocatalytic formic acid (FA) dehydrogenation setup based on Pt(x)@TiO shows stable hydrogen generation activity, which is two orders of magnitude higher than reported values of state-of-the-art systems. Mechanistic studies confirm that hydrogen generation proceeds via a photocatalytic pathway, which is entirely different from purely thermal reaction mechanisms previously reported.

H-driven biocatalysis for flavin-dependent ene-reduction in a continuous closed-loop flow system utilizing H from water electrolysis.

Despite the increasing demand for efficient and sustainable chemical processes, the development of scalable systems using biocatalysis for fine chemical production remains a significant challenge. We have developed a scalable flow system using immobilized enzymes to facilitate flavin-dependent biocatalysis, targeting as a proof-of-concept asymmetric alkene reduction. The system integrates a flavin-dependent Old Yellow Enzyme (OYE) and a soluble hydrogenase to enable H-driven regeneration of the OYE cofactor FMNH.

Nanowired electrodes as outer membrane cytochrome-independent electronic conduit in .

Extracellular electron transfer (EET) from microorganisms to inorganic electrodes is a unique ability of electrochemically active bacteria. Despite rigorous genetic and biochemical screening of the -type cytochromes that make up the EET network, the individual electron transfer steps over the cell membrane remain mostly unresolved. As such, attempts to transplant entire EET chains from native into non-native exoelectrogens have resulted in inferior electron transfer rates.