Multilocus Sequence Analysis and Detection of Copper Ion Resistance of pv. Causing Bacterial Blight in Cassava.

Cassava ( Crantz) is an important tropical tuber crop around the world. Cassava bacterial blight, caused by pv. , is a key disease that influences cassava production worldwide.

Whole genome resequencing reveal patterns of genetic variation within Colletotrichum acutatum species complex from rubber trees in China.

The Colletotrichum acutatum species complex possesses a diverse number of important traits, such as a wide host range and host preference, different modes of reproduction, and different strategies of host infection. Research using comparative genomics has attempted to find correlations between these traits. Here, we used multi-locus techniques and gene genealogical concordance analysis to investigate the phylogenetic relationships and taxonomic status of the Colletotrichum acutatum species complex using field isolates obtained from rubber trees.

Isolation and Identification of Secondary Metabolites Produced by Phytopathogenic Fungus from .

The secondary metabolites of the phytopathogenic fungus CC01 from were investigated. As a result, two new compounds, 5-acetyl-7-hydroxy-6- methoxybenzofuran-2(3)-one () and ()-2-(2,3-dihydrofuro [3,2-]pyridin-2-yl)propan-2-ol (), together with seven known compounds, 4,6,8-trihydroxy-3,4-dihydronaphthalen-1(2)-one (), 3,6,8-trihydroxy-3,4-dihydronaphthalen-1(2)-one (), curvulin acid (), 2-methyl-5-carboxymethyl- 7-hydroxychromone (), tyrosol (), -hydroxybenzoic acid () and cerevisterol (), were isolated from the fermentation extract by comprehensive silica gel, reverse phase silica gel, Sephadex-LH20 column chromatography and high-performance liquid chromatography (HPLC). The structures of these compounds were identified by using high-resolution electrospray mass spectrometry (HRESIMS), nuclear magnetic resonance spectroscopy (NMR), optical rotation, ultraviolet and infrared spectroscopy techniques and a comparison of NMR data with those reported in the literature.

Characterization and necrosis-inducing activity of necrosis- and ethylene-inducing peptide 1-like proteins from , the causative agent of rubber tree anthracnose.

, a member of the species complex, is an important pathogen causing rubber tree anthracnose. Genome-wide comparative analysis showed this species complex contains more genes encoding necrosis- and ethylene-inducing peptide 1-like proteins (NLPs) than other species complexes, but little is known about their necrosis-inducing roles in host. The aim of this study was to analyze NLPs number and type in , and characterize their necrosis-inducing activity in host or non-host.

Antifungal activity of volatile compounds generated by endophytic fungi Sarocladium brachiariae HND5 against Fusarium oxysporum f. sp. cubense.

The soil-born filamentous fungal pathogen Fusarium oxysporum f. sp. cubense (FOC), which causes vascular wilt disease in banana plants, is one of the most economically important Fusarium species.

Genomic Characteristics and Comparative Genomics Analysis of Two Chinese Strains Causing Corynespora Leaf Fall (CLF) Disease.

Rubber tree leaf fall (CLF) disease, caused by the fungus , is one of the most damaging diseases in rubber tree plantations in Asia and Africa, and this disease also threatens rubber nurseries and young rubber plantations in China. isolates display high genetic diversity, and virulence profiles vary significantly depending on cultivar. Although one phytotoxin (cassicolin) has been identified, it cannot fully explain the diversity in pathogenicity between species, and some virulent strains do not contain the cassiicolin gene.

First Report on Neopestalotiopsis aotearoa of Rubber Tree in China.

Natural rubber is an important industrial raw material and an economically important perennial in China. In recent years, A new leaf fall disease, caused by Neopestalotiopsis aotearoa Maharachch., K.

Pathogenic Adaptations Revealed by Comparative Genome Analyses of Two spp., the Causal Agent of Anthracnose in Rubber Tree.

and cause leaf disease that differ in their symptoms in rubber tree (), and pathogenicity of these two fungal species is also not identical on different cultivars of rubber tree. This divergence is often attributed to pathogen virulence factors, namely carbohydrate-active enzymes (CAZymes), secondary metabolites (SM), and small-secreted protein (SSP) effectors. The draft genome assembly and functional annotation of potential pathogenicity genes of both species obtained here provide an important and timely genomic resource for better understanding the biology and lifestyle of spp.

Genomic characteristics and comparative genomics analysis of the endophytic fungus Sarocladium brachiariae.

Background: Sarocladium brachiariae is a newly identified endophytic fungus isolated from Brachiaria brizantha. A previous study indicated that S. brachiariae had antifungal activity; however, limited genomic information restrains further study.

Colletotrichum Species Causing Anthracnose of Rubber Trees in China.

Anthracnose caused by Colletotrichum is one of the most severe diseases of Hevea brasiliensis. However, research on the diversity and geographical distribution of Colletotrichum remains limited in China. In this study, we investigated the phylogenetic diversity of Colletotrichum isolates associated with symptomatic tissues of H.

Cloning of insertion site flanking sequence and construction of transfer DNA insert mutant library in Stylosanthes colletotrichum.

Stylosanthes sp. is the most important forage legume in tropical areas worldwide. Stylosanthes anthracnose, which is mainly caused by Colletotrichum gloeosporioides, is a globally severe disease in stylo production.

Identifying pathogenicity genes in the rubber tree anthracnose fungus Colletotrichum gloeosporioides through random insertional mutagenesis.

To gain more insight into the molecular mechanisms of Colletotrichum gloeosporioides pathogenesis, Agrobacterium tumefaciens-mediated transformation (ATMT) was used to identify mutants of C. gloeosporioides impaired in pathogenicity. An ATMT library of 4128 C.

HbMyb1, a Myb transcription factor from Hevea brasiliensis, suppresses stress induced cell death in transgenic tobacco.

Tapping panel dryness (TPD) is a complex physiological syndrome found widely in rubber tree (Hevea brasiliensis) plantations that causes severe yield loss in natural rubber-producing countries. In an earlier study, we confirmed that there is a negative correlation between HbMyb1 expression and TPD severity. To further investigate the function of HbMyb1 in TPD, HbMyb1 was over-expressed in tobacco controlled by a CaMV 35S promoter.

[Identification of a hrp-associated gene hpaB in the role of pathogenicity of Xanthomonas oryzae pv. oryzae].

Xanthomonas oryzae pv. oryzae (Xoo), a Gram-negative bacterium, is the causal agent of rice bacterial blight disease, which can cause severe yield loss of rice worldwide. To identify genes contributing to virulence and explore the possible mechanism of pathogenicity, transposon mutagenesis was used to isolate nonpathogenic mutants.

Detecting and differentiating Acremonium implicatum: developing a PCR-based method for an endophytic fungus associated with the genus Brachiaria.

SUMMARY Brachiaria is a pan-tropical genus of grasses with about 100 species. The fungus Acremonium implicatum can develop an endophytic association that is mutually beneficial with Brachiaria species. We developed a polymerase chain reaction (PCR)-based method by first amplifying DNA from A.

Association of decreased expression of a Myb transcription factor with the TPD (tapping panel dryness) syndrome in Hevea brasiliensis.

TPD (tapping panel dryness) is a complex physiological syndrome widely found in rubber tree (Hevea brasiliensis) plantations, which causes severe yield and crop losses in natural rubber-producing countries. The molecular mechanism underlying TPD is not known and there is presently no effective prevention or treatment for this serious disease. To investigate the molecular mechanism of TPD, we isolated and characterized genes for which the change of expression is associated with TPD.