A dual-mode homogeneous electrochemical-colorimetric biosensing sensor for carcinoembryonic antigen detection based on a microfluidic paper-based analysis device.

Dual-mode-based sensors have drawn a lot of interest due to their high accuracy and sensitivity compared to single-response systems. A simple electrochemical and colorimetric dual-mode sensor based on enzyme-linked immunosorbent assay (ELISA), without complex electrode surface modification, was developed for accurate and sensitive detection of carcinoembryonic antigen (CEA). The target CEA is recognized by an antibody coupled to horseradish peroxidase (HRP), which then oxidizes the substrate 3,3',5,5'-tetramethylbenzidine (TMB) to generate both a colorimetric and an electrochemical signal.

Exosome Isolation and Detection: From Microfluidic Chips to Nanoplasmonic Biosensor.

Exosomes are becoming more widely acknowledged as significant circulating indicators for the prognosis and diagnosis of cancer. Circulating exosomes are essential to the development and spread of cancer, according to a growing body of research. Using existing technology, characterizing exosomes is quite difficult.

Prognostic value of extrahepatic metastasis on colon cancer with liver metastasis: a retrospective cohort study.

Introduction: The occurrence of metastasis is a threat to patients with colon cancer (CC), and the liver is the most common metastasis organ. However, the role of the extrahepatic organs in patients with liver metastasis (LM) has not been distinctly demonstrated. Therefore, this research aimed to explore the prognostic value of extrahepatic metastases (EHMs).

Associations of Dietary Intakes with Gynecological Cancers: Findings from a Cross-Sectional Study.

Background: Gynecological cancers, including cervical cancer, ovarian cancer and endometrial cancer are leading causes of cancer-related death in women worldwide. Diet plays an important role in cancer development, which is widely accepted. However, the associations between dietary intakes and gynecological cancers remain unclear.

Cancers and COVID-19 Risk: A Mendelian Randomization Study.

Observational studies have shown increased COVID-19 risk among cancer patients, but the causality has not been proven yet. Mendelian randomization analysis can use the genetic variants, independently of confounders, to obtain causal estimates which are considerably less confounded. We aimed to investigate the causal associations of cancers with COVID-19 outcomes using the MR analysis.

Which Probiotic Is the Most Effective for Treating Acute Diarrhea in Children? A Bayesian Network Meta-Analysis of Randomized Controlled Trials.

Acute diarrhea is a major cause of morbidity and mortality in children under five. Probiotics are beneficial for treating acute diarrhea in children, but unclear which specific probiotic is the most effective. We performed a Bayesian network meta-analysis to examine the comparative effectiveness of probiotics.

The active fragments of ghrelin cross the blood-brain barrier and enter the brain to produce antinociceptive effects after systemic administration.

G (1-5)-NH, G (1-7)-NH, and G (1-9) are the active fragments of ghrelin. The aim of this study was to investigate the antinociceptive effects, their ability to cross the blood-brain barrier, and the receptor mechanism(s) of these fragments using the tail withdrawal test in male Kunming mice. The antinociceptive effects of these fragments (2, 6, 20, and 60 nmol/mouse) were tested at 5, 10, 20, 30, 40, 50, and 60 min after intravenous (i.

Wnt/β‑catenin signaling: Causes and treatment targets of drug resistance in colorectal cancer (Review).

Colorectal cancer (CRC) is the third most common malignant tumor in humans. Chemotherapy is used for the treatment of CRC. However, the effect of chemotherapy remains unsatisfactory due to drug resistance.

Synthesis of Gold Nanoparticle Stabilized on Silicon Nanocrystal Containing Polymer Microspheres as Effective Surface-Enhanced Raman Scattering (SERS) Substrates.

Developing ideal surface-enhanced Raman scattering (SERS) substrates is significant in biological detection. Compared with free non-aggregated noble metal nanoparticles, loading metal nanoparticles on a large matrix can achieve a higher SERS effect due to the existence of many "hot spots". A novel SERS substrate with intense "hot spots" was prepared through reducing gold ions with silicon nanocrystal containing polymer microspheres.

A metal-organic zeolitic framework with immobilized urease for use in a tapered optical fiber urea biosensor.

A tapered single-mode coreless single-mode (SCS) structure with high sensitivity for sensing refractive index is described. In order to achieve high specificity of optical biosensors, here enzyme capsulation film was achieved by embedding urease in zeolitic imidazolate framework (ZIF-8/urease) through in situ growth approach on the coreless fibers. Determination of urea is achieved through online monitoring of its binding to the urease in zeolitic imidazolate framework.

Octadecylamine degradation through catalytic activation of peroxymonosulfate by FeMn layered double hydroxide.

A kind of heterogeneous catalyst, FeMn layered double hydroxide (Fe-Mn-LDH), was fabricated by coprecipitation process and used as PMS activator to degrade a novel organic pollutant octadecylamine (ODA). And the X-ray diffraction (XRD), scanning electron microscope (SEM), transmission electron microcopy (TEM), Mapping and X-ray photoelectron spectra (XPS) measurements were utilized to characterize the fresh and used Fe-Mn-LDH. After a serious of degradation experiments, it was clearly to see that the activator possessed excellent activation property for PMS and was capable of removing 85% ODA (10 mg·L) within 25 min obviously higher than pure PMS.

Microfluidic device for the analysis of MDR cancerous cell-derived exosomes' response to nanotherapy.

Exosomes are membrane-enclosed extracellular vesicles which have been indicated as important biomarkers of cancerous cell functionality, such as multiple drug resistance (MDR). Nanoparticles based chemotherapy is a promising strategy to overcome MDR by interfering the production and composition of exosomes. Therefore, tumor-derived exosomes post-treatment by nanotherapy are implied to play critical roles of biomarkers on cancer MDR analysis.

Enzyme-Embedded Metal-Organic Framework Colloidosomes via an Emulsion-Based Approach.

Improving the activity and stability of enzymes is significant in enzyme immobilization. Here a facile approach to prepare ring-like ZIF-8 colloidosomes and spherical catalase-embedded ZIF-8 colloidosomes is developed via one-step emulsion-based technique at the water/butanol interface. The influence of the concentrations of ZIF-8 nanocrystals and Pluronic F127 as well as the oil-water ratio was investigated.

Silver-Nanoparticle-Embedded Porous Silicon Disks Enabled SERS Signal Amplification for Selective Glutathione Detection.

As the major redox couple and nonprotein thiol source in human tissues, the level of glutathione (GSH) has been a concern for its relation with many diseases. However, the similar physical and chemical properties of interference molecules such as cysteine (Cys) and homocysteine (Hcy) make discriminative detection of GSH in complex biological fluids challenging. Here we report a novel surface-enhanced Raman scattering (SERS) platform, based on silver-nanoparticle-embedded porous silicon disks (PSDs/Ag) substrates for highly sensitive and selective detection of GSH in biofluids.

Multi-step encapsulation of chemotherapy and gene silencing agents in functionalized mesoporous silica nanoparticles.

Drug to carrier ratio is an important consideration in designing drug platforms, since a low loading capacity necessitates the use of high doses of carriers, which can result in side effects. Here, we have engineered a platform to co-deliver small molecule drugs and small interfering RNA (siRNA). This platform consists of cyclodextrin-grafted polyethylenimine (CP) functionalized mesoporous silica nanoparticles (MSNP).

Correction: In situ growth of fluorescent silicon nanocrystals in a monolithic microcapsule as a photostable, versatile platform.

Correction for 'In situ growth of fluorescent silicon nanocrystals in a monolithic microcapsule as a photostable, versatile platform' by Guixian Zhu, et al., Nanoscale, 2016, 8, 15645-15657.

In situ growth of fluorescent silicon nanocrystals in a monolithic microcapsule as a photostable, versatile platform.

A facile, one-step method was developed for the in situ formation of fluorescent silicon nanocrystals (SiNC) in a microspherical encapsulating matrix. The obtained SiNC encapsulated polymeric microcapsules (SiPM) possess uniform size (0.1-2.

A versatile quantitation platform based on platinum nanoparticles incorporated volumetric bar-chart chip for highly sensitive assays.

Platinum nanoparticles incorporated volumetric bar-chart chip (PtNPs-V-Chip) is able to be used for point-of-care tests by providing quantitative and visualized readout without any assistance from instruments, data processing, or graphic plotting. To improve the sensitivity of PtNPs-V-Chip, hybridization chain reaction was employed in this quantitation platform for highly sensitive assays that can detect as low as 16 pM Ebola Virus DNA, 0.01ng/mL carcinoembryonic antigen (CEA), and the 10 HER2-expressing cancer cells.

In Situ Reductive Synthesis of Structural Supported Gold Nanorods in Porous Silicon Particles for Multifunctional Nanovectors.

Porous silicon nanodisks (PSD) were fabricated by the combination of photolithography and electrochemical etching of silicon. By using PSD as a reducing agent, gold nanorods (AuNR) were in situ synthesized in the nanopores of PSD, forming PSD-supported-AuNR (PSD/AuNR) hybrid particles. The formation mechanism of AuNR in porous silicon (pSi) was revealed by exploring the role of pSi reducibility and each chemical in the reaction.