The novel cathelicidin-DM antimicrobial peptide conjugated carbomer and thermosensitive chitosan hydrogel speeds up wound-healing in both non-infected and S. aureus-infected wounds.

The emergence of chronic wound infections and bacterial resistance presents substantial clinical challenges that impact millions worldwide. Antimicrobial peptides (AMPs), recognized for their potent antimicrobial properties, are considered promising alternatives to conventional antibiotics in light of escalating drug resistance. In previous research, we isolated an AMP named cathelicidin-DM from Duttaphrynus melanostictus, which exhibited broad-spectrum efficacy against multidrug-resistant bacteria and demonstrated wound-healing capabilities.

Population genomics reveals demographic history and selection signatures of hazelnut ().

Hazelnut ( spp.) is known as one of the four famous tree nuts in the world due to its pleasant taste and nutritional benefits. However, hazelnut promotion worldwide is increasingly challenged by global climate change, limiting its production to a few regions.

Plastome phylogenomics provide new perspective into the phylogeny and evolution of Betulaceae (Fagales).

Background: Betulaceae is a relatively small but morphologically diverse family, with many species having important economic and ecological values. Although plastome structure of Betulaceae has been reported sporadically, a comprehensive exploration for plastome evolution is still lacking. Besides, previous phylogenies had been constructed based on limited gene fragments, generating unrobust phylogenetic framework and hindering further studies on divergence ages, biogeography and character evolution.

Wound healing mechanism of antimicrobial peptide cathelicidin-DM.

Chronic wound infections and the development of antibiotic resistance are serious clinical problems that affect millions of people worldwide. Cathelicidin-DM, an antimicrobial peptide from , has powerful antimicrobial activity and wound healing efficacy. So, it could be a potential candidate to address this problem.

Stigmatic Transcriptome Analysis of Self-Incompatible and Compatible Pollination in Fisch. × L.

Self-incompatibility (SI) protects plants from inbreeding depression due to self-pollination and promotes the outcrossing process to maintain a high degree of heterozygosity during evolution. is an important woody oil and nut species that shows sporophytic SI (SSI). Yet the molecular mechanism of SI in remains largely unknown.

Species divergence and phylogeography of Corylus heterophylla Fisch complex (Betulaceae): Inferred from molecular, climatic and morphological data.

Historical geo-climatic changes have shaped the geographical distributions and genetic diversity of numerous plant taxa in East Asia, which promote species divergence and ultimately speciation. Here, we integrated multiple approaches, including molecular phylogeography, ecological niche modeling, and morphological traits to examine the nucleotide diversity and interspecific divergence within Corylus heterophylla complex (C. heterophylla, C.

Characteristics of the Fungal Communities and Co-occurrence Networks in Hazelnut Tree Root Endospheres and Rhizosphere Soil.

Hazelnut has gained economic value in China in recent years, but its large-scale planting and research started later than other countries. Conducting basic research on hazelnut trees requires studying their related microorganisms. Here, we used high-throughput DNA sequencing to quantify the fungal communities in the root endospheres and rhizosphere soil of four hazelnut species.

Seasonal Changes in Soil Microbial Community and Co-Occurrence Network of Species of the Genus .

Hazelnut is one of the four major nuts in the world and has high nutritional and economic value. This study employed Illumina sequencing of ITS rDNA and 16S rRNA genes to identify the seasonal changes in soil microbial community, the predominant environmental factors driving microbial community composition, and the differences in soil microbial composition among different species of the genus . We found that the soil microbial community composition of species of changed significantly with the change in seasons.

A chromosome-level reference genome of the hazelnut, Corylus heterophylla Fisch.

Background: Corylus heterophylla Fisch. is a species of the Betulaceae family native to China. As an economically and ecologically important nut tree, C.

Effects of Living Cover on the Soil Microbial Communities and Ecosystem Functions of Hazelnut Orchards.

Living cover is an important management measure for orchards in China, and has certain influences on soil properties, microorganisms, and the micro-ecological environment. However, there are few studies on the effects of living cover on the soil changes in hazelnut orchards. In this study, we compared the soils of living cover treatments with and the soils of no cover treatments, and analyzed the observed changes in soil properties, microorganisms, and microbial functions by using high-throughput ITS rDNA and 16S rRNA gene Illumina sequencing.

Selection and Validation of Reference Genes for Quantitative RT-PCR Analysis in Fisch. L.

(1) Background: the species of have sporophytic type of self-incompatibility. Several genes related to recognition reaction between pollen and stigma have been identified in hazelnuts. To better understand the self-incompatibility (SI) response, we screened the suitable reference genes by using quantitative real-time reverse transcription PCR (qRT-PCR) analysis in hazelnut for the first time.

Molecular cloning and expression analysis of hybrid hazelnut (Corylus heterophylla × Corylus avellana) ChaSRK1/2 genes and their homologs from other cultivars and species.

The self-incompatibility system of Corylus is a sporophytic type that is phenotypically similar to that of Brassica. While the molecular mechanism of sporophytic self-incompatibility (SSI) has been investigated extensively in Brassica (Brassicaceae), little is known about the corresponding mechanism in Corylus (Betulaceae). Here, we discuss the SSI mechanism with respect to S-locus receptor kinase (SRK) gene homologs.

Multilocus data reveal deep phylogenetic relationships and intercontinental biogeography of the Eurasian-North American genus Corylus (Betulaceae).

The evolutionary history of the genus Corylus, a tertiary disjunct lineage consisting of approximately 15-20 taxa with New and Old World distribution, has not been fully studied using molecular tools. In this research, we reconstructed comprehensive phylogenies of this genus using multiple datasets (genome-wide SNPs; complete chloroplast genomes; and nuclear ribosomal ITS sequences) based on detailed sampling of 17 Corylus species currently recognized. Divergence times were estimated using a fossil calibrated relaxed clock model, and ancestral area reconstruction were inferred using Bayesian binary MCMC (BBM) method and the dispersal-extinction-cladogenesis (DEC) model.

The complete chloroplast genomes of three Betulaceae species: implications for molecular phylogeny and historical biogeography.

Background: Previous phylogenetic conclusions on the family Betulaceae were based on either morphological characters or traditional single loci, which may indicate some limitations. The chloroplast genome contains rich polymorphism information, which is very suitable for phylogenetic studies. Thus, we sequenced the chloroplast genome sequences of three Betulaceae species and performed multiple analyses to investigate the genome variation, resolve the phylogenetic relationships, and clarify the divergence history.

Resolving the Speciation Patterns and Evolutionary History of the Intercontinental Disjunct Genus (Betulaceae) Using Genome-Wide SNPs.

Understanding the underlying mechanisms of species origin, divergence, and distribution patterns of the intercontinental disjunct taxa has long fascinated botanists. Based on 4,894 genome-wide single-nucleotide polymorphism dataset, we present a molecular phylogenetic reconstruction of genus (Betulaceae), which have a disjunct distribution between Eurasia and North America (NA). The aim is to explore the speciation patterns and evolutionary relationships of species by establishing a general phylogenetic framework with extensive sampling.

Comparative Genomics and Phylogenetic Analysis Revealed the Chloroplast Genome Variation and Interspecific Relationships of (Betulaceae) Species.

L. is an economically and phylogenetically important in the family Betulaceae. Taxonomic and phylogenetic relationships of species have long been controversial for lack of effective molecular markers.

Assessment of Genetic Diversity and Population Genetic Structure of Corylus mandshurica in China Using SSR Markers.

Corylus mandshurica, also known as pilose hazelnut, is an economically and ecologically important species in China. In this study, ten polymorphic simple sequence repeat (SSR) markers were applied to evaluate the genetic diversity and population structure of 348 C. mandshurica individuals among 12 populations in China.

Expression and Functional Analysis of WRKY Transcription Factors in Chinese Wild Hazel, Corylus heterophylla Fisch.

Plant WRKY transcription factors are known to regulate various biotic and abiotic stress responses. In this study we identified a total of 30 putative WRKY unigenes in a transcriptome dataset of the Chinese wild Hazel, Corylus heterophylla, a species that is noted for its cold tolerance. Thirteen full-length of these ChWRKY genes were cloned and found to encode complete protein sequences, and they were divided into three groups, based on the number of WRKY domains and the pattern of zinc finger structures.

[Effects of saline-alkali stress on electrical impedance spectroscopy parameters and ion contents in shoots of Ping'ou hybrid hazelnut].

To study the adaptability to salt-alkaline stress of Ping'ou hybrid hazelnut, 'Liaozhen 3' shoots which were treated with three types of stress neutral NaCl, alkaline Na2CO3, and mixed salt-alkali, and the changes in electrical impedance spectroscopy (EIS) parameters and mineral ion contents were subsequently determined. The correlations between the EIS parameters and mineral ion contents were analyzed. The results showed that with the increasing level of NaCl, specific high- frequency resistance (r), specific low-frequency resistance ( r(l)), specific intracellular resistance (r(i)) and specific extracellular resistance (r(e)) of shoots decreased firstly, then increased, and finally decreased again.

Transcriptome sequencing and identification of cold tolerance genes in hardy Corylus species (C. heterophylla Fisch) floral buds.

Background: The genus Corylus is an important woody species in Northeast China. Its products, hazelnuts, constitute one of the most important raw materials for the pastry and chocolate industry. However, limited genetic research has focused on Corylus because of the lack of genomic resources.

Vascular anatomy of kiwi fruit and its implications for the origin of carpels.

Kiwi fruit is of great agricultural, botanical, and economic interest. The flower of kiwi fruit has axile placentation, which is typical for Actinidiaceae. Axile placentation is thought derived through fusion of conduplicate carpels with marginal placentation according to the traditional doctrine.

[Methylenetetrahydrofolate reductase and methionine synthase reductase gene polymorphisms in ethnic Han women from Linyi].

Objective: To explore the distribution of genetic polymorphisms of methylenetetrahydrofolate reductase (MTHFR) 677C/T, 1298A/C and methionine synthase reductase (MTRR) 66A/G among ethnic Han females from Linyi, and to correlate it with serum level of homocysteine (Hcy).

Methods: A cross-sectional study was carried out. Oral epithelial cell samples were collected from 825 subjects.

Effect of pre-pregnancy body mass index on adverse pregnancy outcome in north of China.

Purpose: To investigate the effect of pre-pregnancy BMI on adverse maternal and neonatal outcomes in pregnancy women of northern China.

Methods: We conducted a retrospective population-based cohort study of 5,047 singleton nulliparous pregnancies. The subjects were categorized into four groups by BMI-underweight (BMI < 18.

Highly sensitive and spatially resolved polyvinyl alcohol/acrylamide photopolymer for real-time holographic applications.

By employing low molecular-weight polyvinyl alcohol (PVA) as binder, the spatial resolution of a red-sensitive PVA/acrylamide based photopolymer are improved from 1000 lines/mm to 3000 lines/mm. By increasing the ambient temperature during the holographic recording, the photosensitivity of photopolymer is also increased about 5 times. The optimized photopolymer system has high capacity such as high photosensitivity (8 mJ/cm(2)), high spatial resolution (over 3000 lines/mm) and high diffraction efficiency (over 94%).