Publications by authors named "Guiting Kang"
Article Synopsis
- Researchers investigated a new method called PRIME-Del (PDel) for precise genomic deletions in plants, achieving an average deletion efficiency of 55.8% for small DNA fragments and up to 84.2% for larger deletions in rice.
- The study also introduced a combined strategy, PDel/Syn, which utilized base mutations to correct unintended deletions, resulting in an average deletion efficiency of 58.1% at multiple genomic targets.
- These innovative strategies aim to improve the precision of genetic modifications in transgenic rice, potentially enhancing agricultural traits and research into protein functions.
The ability to manipulate the genome in a programmable manner has illuminated biology and shown promise in plant breeding. Prime editing, a versatile gene-editing approach that directly writes new genetic information into a specified DNA site without requiring double-strand DNA breaks, suffers from low efficiency in plants. In this study, N-terminal reverse transcriptase-Cas9 nickase fusion performed better in rice than the commonly applied C-terminal fusion.
View Article and Find Full Text PDFThe CRISPR-Cas9 system enables simple, rapid, and effective genome editing in many species. Nevertheless, the requirement of an NGG protospacer adjacent motif (PAM) for the widely used canonical Cas9 (SpCas9) limits the potential target sites. The xCas9, an engineered SpCas9 variant, was developed to broaden the PAM compatibility to NG, GAA, and GAT PAMs in human cells.
View Article and Find Full Text PDFThe development of CRISPR/Cas9-mediated base editing has made genomic modification more efficient. However, selection of genetically modified cells from millions of treated cells, especially plant cells, is still challenging. In this study, an efficient surrogate reporter system based on a defective hygromycin resistance gene was established in rice to enrich base-edited cells.
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