Environmental Factors Drive the Biogeographic Pattern of Root Endophytic Fungal Diversity in the Arid Regions of Northwest China.

subsp. Rousi (Abbrev. ) stands as a vital botanical asset in ameliorating the ecological landscape of the arid regions in Northwest China, where its rhizospheric microorganisms serve as linchpins in its growth and developmental dynamics.

Effects of Tibetan Sheep-Derived Compound Probiotics on Growth Performance, Immune Function, Intestinal Tissue Morphology, and Intestinal Microbiota in Mice.

Probiotics play an important role in animal growth, immunity, and gut microbial balance and are now widely used in agriculture, food, and medicine. This study analysed the effects of different concentrations of Tibetan sheep compound probiotics on the immunity, tissue morphology, and intestinal microbiota of mice using histological, molecular, and 16S rRNA techniques. The results showed that the composite probiotics sourced from Tibetan sheep improved the growth performance of mice, increased the length of small intestinal villi and mucosal thickness, and enhanced the intestinal barrier function of mice.

Development and application of microsatellite markers in subsp. Rousi ( L.) based on transcriptome sequencing.

subsp. Rousi is a cold- and drought-tolerant pioneer species with significant economic and ecological value. Evaluating its genetic diversity and population structure is of great importance for guiding the development and utilization of resources.

Exploring effect of herbal monomers in treating gouty arthritis based on nuclear factor-kappa B signaling: A review.

Gouty arthritis (GA) is an inflammatory disease caused by disorders of the purine metabolism. Although increasing number of drugs have been used to treat GA with the deepening of relevant research, GA still cannot be cured by simple drug therapy. The nuclear factor-kappa B (NF-κB) signaling pathway plays a key role in the pathogenesis of GA.

Effects of three different probiotics of Tibetan sheep origin and their complex probiotics on intestinal damage, immunity, and immune signaling pathways of mice infected with type C.

Tibetan sheep have unique intestinal microorganisms in their intestines that are adapted to the highland alpine and anoxic environment. To further clarify the probiotic properties of Tibetan sheep-derived probiotics, we selected three Tibetan sheep-derived probiotic isolates ( EF1-mh, BS1-ql, and LS-ql) to investigate the protective mechanisms of monocultures and their complex strains against type C infection in mice. We established a model of type C infection and used histology and molecular biology to analyze the effects and mechanisms of different probiotic treatments on mice after type C infection.

Ultrathin ZIF-8 wrapping on Au-dotted Ag-nanowires for highly selective SERS-based CO gas detection.

Ultrathin ZIF-8 wrapping was constructed on Au-dotted Ag-nanowires to obtain Ag@Au@ZIF-8. A thin film (Ag@Au@ZIF-8 NWs/TF) was constructed to selectively adsorb and enrich CO2 molecules, which solved the problem that SERS signals could not be detected due to the poor affinity of the metal surface and the low concentration of gas molecules.

Transcriptomic analysis of drought stress responses of sea buckthorn (Hippophae rhamnoidessubsp. sinensis) by RNA-Seq.

Sea buckthorn is one of the most important eco-economic tree species in China due to its ability to grow and produce acceptable yields under limited water and fertilizer availability. In this study, the differentially expressed genes under drought stress (DS) of sea buckthorn were identified and compared with control (CK) by RNA-Seq. A total of 122,803 unigenes were identified in sea buckthorn, and 70,025 unigenes significantly matched a sequence in at least one of the seven databases.

Determination of multidrug resistance mechanisms in Clostridium perfringens type A isolates using RNA sequencing and 2D-electrophoresis.

In this study, we screened differentially expressed genes in a multidrug-resistant isolate strain of Clostridium perfringens by RNA sequencing. We also separated and identified differentially expressed proteins (DEPs) in the isolate strain by two-dimensional electrophoresis (2-DE) and mass spectrometry (MS). The RNA sequencing results showed that, compared with the control strain, 1128 genes were differentially expressed in the isolate strain, and these included 227 up-regulated genes and 901 down-regulated genes.

Morphological Observation and Comparative Transcriptomic Analysis of Clostridium perfringens Biofilm and Planktonic Cells.

Bacterial biofilms can enhance survival in adverse environments and promote infection. However, little is known about biofilm formation by Clostridium perfringens. To better characterize this process, we used SEM to observe the surfaces of C.

[Phylogenetic relationships of seabuckthorn based on ITS sequences].

Sequences of the internal transcribed spacer (ITS) was compared in three seabuckthrons (Hippophae rhamnoides subsp. sinensis, H. tibetana and H.

Oral administration of tepoxalin in the horse: a PK/PD study.

Tepoxalin is a non-steroidal anti-inflammatory drug with analgesic, anti-inflammatory, and antipyretic properties and has been recently introduced into veterinary medicine. The aim of this study was to evaluate the pharmacokinetic/pharmacodynamic (PK/PD) profile of tepoxalin to assess whether it would be suitable for clinical use in horses. Six female fasting/fed horses were given 10mg/kg tepoxalin orally in a cross-over study.

Cytopathic effect of classical swine fever virus NS3 protein on PK-15 cells.

In order to further research the relationship between classical swine fever virus' (CSFV) NS3 protein and the cytopathic effect (CPE) in cells infected with the CSFV, and to reveal the effect of protein NS3 on the host cells, the NS3 of CSFV Shimen strain amplified by RT-PCR was subcloned into the pEGFP-C1, named pEGFP-C1-NS3. The insert position, the size and the reading frame were correct for restriction enzyme digestion and sequence analysis. The pEGFP-C1-NS3 and pEGFP-C1 were transfected into PK-15 cells by liposome, and positive cell clones were gained by G418.