Effect of 5-Aza-2'-deoxycytidine on T-cell acute lymphoblastic leukemia cell biological behaviors and PTEN expression.

Objective: We currently face a sharp increase of T-cell acute lymphoblastic leukemia (T-ALL) incidence and a challenge of unmasking its complex etiology. The deoxycytidine analog 5-Aza-2'-deoxycytidine (5-Aza-dC) is currently the most common nucleoside methyltransferase inhibitor. The objective of this study was to clarify the role of 5-Aza-dC in T-ALL cell biological behaviors and phosphatase and tensin homolog deleted on chromosome ten (PTEN) expression.

Single-cell sequencing technology to characterize stem T-cell subpopulations in acute T-lymphoblastic leukemia and the role of stem T-cells in the disease process.

Background: Precursor T-cell acute lymphoblastic leukemia (Pre-T ALL) is a malignant neoplastic disease in which T-cells proliferate in the bone marrow. Single-cell sequencing technology could identify characteristic cell types, facilitating the study of the therapeutic mechanisms in Pre-T ALL.

Methods: The single-cell sequencing data (scRNA-seq) of Pre-T ALL were obtained from public databases.

[The Expression and Correlation of , and Calreticulin in Diffuse Large B-Cell Lymphoma].

Objective: To analyze the expression and correlation of microRNA-195 (miR-195), and calreticulin in diffuse large B-cell lymphoma (DLBCL).

Methods: From April 2020 to April 2021, 80 DLBCL patients with complete data archived by the Pathology Department of Handan First Hospital and The Second Hospital of Hebei Medical University were selected as the study group, and 70 patients with reactive lymph node hyperplasia were selected as the control group. The expressions of and were detected by real-time fluorescence quantitative PCR, and the expression of calreticulin was detected by Western blot.

[Relationship between Polymorphism of miR-155 and Its Target Gene MyD88 and Clinicopathological Features of Diffuse Large B-cell Lymphoma].

Objective: To investigate the relationship between the polymorphism of miR-155 and its target gene MyD88 and clinicopathological features of diffuse large B-cell lymphoma (DLBCL).

Methods: 135 cases of DLBCL patients in our hospital from March 2015 to August 2017 were selected, and 90 cases of reactive hyperplasia of lymph nodes were selected as the control group. The relative expression of miR-155 and MyD88 gene polymorphism were detected in the two groups, and the relationship between miR-155 and MyD88 gene polymorphism and clinicopathological characteristics of DLBCL was analyzed.

TUC338 Promotes Diffuse Large B Cell Lymphoma Growth via Regulating EGFR/PI3K/AKT Signaling Pathway.

TUC338 is emerging as a novel vital long noncoding RNA (lncRNA) in human cancer; however, its role in diffuse large B cell lymphoma (DLBCL) remains unknown. In this study, we found that TUC338 was remarkably upregulated in DLBCL tissues as compared to matched normal tissues. High TUC338 was closely related to advanced Ann Arbor stage, resistance to CHOP-like treatment, and high IPI (International Prognostic Index).

[Effects of Long Non-coding RNA-TUC338 on the Migration and Proliferation of Lymphoma Cells via PI3K/AKT Signaling Pathway].

Objective: To investigate the effect of long non-coding RNA-TUC338 on the proliferation and migration of lymphoma cells.

Methods: The expression of TUC338 in different lymphoma cells was detected by fluorescence quantitative PCR, cell proliferation by sulforhodamine B (SRB) assay, migration of lymphoma cells by transwell assay, and protein expression in PI3K/AKT signaling pathway by Western blot.

Results: The expression levels of TUC338 in lymphoma cells Daudi, U937, BC-3, and Raji significantly increased in comparison with human normal T lymphocytes H9 (t=13.

[Expression and Clinical Significance of LncRNA KCNQ1OT1 in Patients with Acute Myeloid Leukemia].

Objective: To investigate the expression of LncRNA KCNQ1OT1 in patients with acute myeloid leukemia (AML) and to analyze the relation of LncRNA KCNQ1OT1 expression levels with clinicopathological features.

Methods: A total of 68 patients with AML were enrolled in the study, 48 out of them were suffered from acute myeloid leukemia (AML) and 20 reached to complete remission (CR), 30 age-matched patients with iron-deficient anemia were included in control group, the peripheral blood samples of all the patients were collected, and the real-time fluorescent quantitative PCR (qRT-PCR) was used to detect the expression of LncRNA KCNQ1OT1, meanwhile, the correlation of its expression with clinicopathological characteristics and prognosis was analyzed.

Results: The expression of LncRNA KCNQ1OT1 in AML patients was significantly higher than that in the patient with complete remission and iron-deficient anemia (F=14.

Synthesis of a ferric hydroxide-coated cellulose nanofiber hybrid for effective removal of phosphate from wastewater.

Ferric hydroxide-coated cellulose nanofibers (Fe(OH)3@CNFs) were synthesized for the removal of phosphate from wastewater. The maximum sorption capacity of Fe(OH)3@CNFs for phosphate was estimated to be 142.86mg/g, demonstrating a superior adsorption capacity compared with many adsorbents reported in the literature.

Effects of ionic liquids on the characteristics of synthesized nano Fe(0) particles.

In this paper, high specific surface area zerovalent Fe(0) nanoparticles with unusual morphology and high reductive activity were prepared using a chemical reductive reaction of iron chloride hydrate with sodium borohydride in an aqueous solution of ionic liquids (ILs). The ionic liquids, [C(4)mim]X (X = Cl(-), Br(-), BF(4)(-), PF(6)(-)) and [C(n)mim][BF(4)] (n = 4, 6, 8), were used for this purpose. Transmission electron microscopy, X-ray diffraction, and Brunauer-Emmett-Teller surface area measurements were used to characterize the morphology, structure, and physical properties of the as-prepared Fe(0).