Screening active components from for pancreatic β-cells protection.

Context: species (Rosaceae) have been used in folk medicine to treat diabetes due to their hypoglycaemic activity.

Objective: To screen the active components that act as hypoglycaemic agents in Focke and the underlying mechanisms.

Materials And Methods: Aqueous stem extract of was incubated with MIN6 β-cells, PBS was used as the blank control.

Separation mechanism of oleanane and ursane pentacyclic triterpenoid isomers by coordination chromatography.

This paper focuses on the study of the separation mechanism of oleanane and ursane pentacyclic triterpenoid isomers by a coordination chromatographic method. Based on the calculation analysis, beta-cyclodextrin (beta-CD) and its derivatives were selected as the suitable agents. The experimental results showed that the resolution of madecassoside isomers with the addition of glucosyl-beta-cyclodextrin (Glu-beta-CD) to the mobile phase (11.

Separation rule of oleanane and ursane pentacyclic triterpenoids isomers from nature plants by coordination chromatography.

Many of oleanolic and ursolic pentacyclic triterpenoid isomers generally coexist. There is a small difference in their structures. Based on coordination chromatography theory, a reversed-phase high-performance thin-layer chromatography (HPLC) method has been investigated for improving the isomers' resolution by adding suitable agents in mobile phase, and the separation rule was summarized.

[Optimization of culture conditions for production of extracellular polysaccharides by Morchella esculenta].

Objective: To optimize the conditions for exopolysaccharides (EPS) production by Morchella esculenta GIM 5.69.

Methods: A Plackett-Burman design was used to evaluate the effects of variables factors.

Isolation, partial characterisation and immunomodulatory activities of polysaccharide from Morchella esculenta.

Background: Polysaccharides extracted from fungi or algae have shown a variety of medical activities, and the exploitation of polysaccharides for application in pharmacy has been very promising. In this study, the immunomodulatory properties of polysaccharides from Morchella esculenta were investigated to identify immunostimulatory activity and potentially contribute to the therapeutic potential of Morchella esculenta.

Results: A water-soluble polysaccharide, MEP, was obtained from the fermentation broth of Morchella esculenta.

[Investigation on the convertion between ginkgolide B and its derivative].

A derivative decomposed from ginkgolide B (GB) was isolated by semi-preparative chromatography. The results of the high performance liquid chromatography (HPLC) showed that the retention time of the derivative was approximately three times of that of GB with ultraviolet detection (UV), and the derivative can not exist independently without the presence of GB. The UV spectrum showed that the lamdamax of the derivative was 212.

[Separation and determination of madecassic acid in triterpenic genins of Centella asiatica by high performance liquid chromatography using beta-cyclodextrin as mobile phase additive].

Centella asiatica (L. ) Urban is a tropical medicinal plant with a long history of therapeutic uses. Madecassic acid and terminolic acid, which are a pair of structural isomers, are two constituents of Centella asiatica.