Label-free multiphoton imaging of -amyloid plaques in Alzheimer's disease mouse models.

-Amyloid ( ) plaque, representing the progressive accumulation of the protein that mainly consists of , is one of the prominent pathological hallmarks of Alzheimer's disease (AD). Label-free imaging of plaques holds the potential to be a histological examination tool for diagnosing AD. We applied label-free multiphoton microscopy to identify extracellular plaque as well as intracellular accumulation for the first time from AD mouse models.

Automated label-free detection of injured neuron with deep learning by two-photon microscopy.

Stroke is a significant cause of morbidity and long-term disability globally. Detection of injured neuron is a prerequisite for defining the degree of focal ischemic brain injury, which can be used to guide further therapy. Here, we demonstrate the capability of two-photon microscopy (TPM) to label-freely identify injured neurons on unstained thin section and fresh tissue of rat cerebral ischemia-reperfusion model, revealing definite diagnostic features compared with conventional staining images.

Polarization-independent Fabry-Perot interferometer in a hole-type silicon photonic crystal.

We propose and numerically demonstrate a polarization-independent Fabry-Perot interferometer (PI-FPI) based on the self-collimation effect in a hole-type silicon photonic crystal (PhC). By use of the polarization peak matching method, a resonance frequency of the transverse-electric modes can equal that of the transverse-magnetic modes in the PI-FPI, although the transmission spectra are quite polarization dependent due to birefringence of the PhC. For the operating wavelength of 1550 nm, the PI free spectral range of the PI-FPI is up to 32.