SAGE transcript profiling of the juvenile cambial region of Eucalyptus grandis.

Despite the importance of Eucalyptus spp. in the pulp and paper industry, functional genomic approaches have only recently been applied to understand wood formation in this genus. We attempted to establish a global view of gene expression in the juvenile cambial region of Eucalyptus grandis Hill ex Maiden.

Comparison of the expression profiles of susceptible and resistant Eucalyptus grandis exposed to Puccinia psidii Winter using SAGE.

Eucalyptus grandis Hill ex Maiden and its hybrids are commonly planted by the Brazilian pulp and paper industry, but they are the most susceptible to the neotropical rust disease caused by Puccinia psidii Winter. In an initial attempt to understand the mechanisms of resistance, we constructed two contrasting Serial Analysis of Gene Expression (SAGE) libraries using susceptible and resistant individuals from a segregating half-sibling E. grandis population.