Translational Regulation of Duplicated Gene Expression Evolution in Allopolyploid Cotton.

Polyploidy, a prevalent event in plant evolution, drives phenotypic diversification and speciation. While transcriptional changes and regulation in polyploids have been extensively studied, the translational level impact remains largely unexplored. To address this gap, we conducted a comparative transcriptomic and translatomic analysis of cotton leaves from allopolyploid species (AD) and (AD) relative to their model A-genome and D-genome diploid progenitors.

Programmable RNA N-methyladenosine editing with CRISPR/dCas13a in plants.

N-methyladenonsine (mA) is the most prevalent internal modification of messenger RNA (mRNA) and plays critical roles in mRNA processing and metabolism. However, perturbation of individual mA modification to reveal its function and the phenotypic effects is still lacking in plants. Here, we describe the construction and characterization of programmable mA editing tools by fusing the mA writers, the core catalytic domain of the MTA and MTB complex, and the AlkB homologue 5 (ALKBH5) eraser, to catalytically dead Cas13a (dCas13a) to edit individual mA sites on mRNAs.