Response surface optimization of ultrasound-assisted enzymatic extraction polysaccharides from Lycium barbarum.

In this study, an efficient ultrasound-assisted enzymatic extraction procedure for the water-soluble polysaccharides from the fruit of Lycium barbarum was investigated and optimized. Response surface methodology (RSM) based on a three-level four-factor Box Behnken Design (BBD) was employed to optimize the extraction conditions inlcluding extraction time, ultrasonic output power, cellulose concentration and extraction temperature. The experimental data were adequately fitted into a second-order polynomial model.

Drying effects on the antioxidant properties of polysaccharides obtained from Agaricus blazei Murrill.

Three polysaccharides (ABMP-F, ABMP-V, ABMP-A) were obtained from Agaricus blazei Murrill via methods such as freeze drying, vacuum drying and air drying, respectively. Their chemical compositions were examined, and antioxidant activities were investigated on the basis of assay for hydroxyl radical, DPPH radical, ABTS free radical scavenging ability and assay for Fe(2+)-chelating ability. Results showed that the three ABMPs have different physicochemical and antioxidant properties.

Effects of extraction methods on the antioxidant activities of polysaccharides from Agaricus blazei Murrill.

Five polysaccharides were obtained from Agaricus blazei Murrill (ABM) through different extraction methods including hot water extraction, single enzyme extraction (pectinase, cellulase or papain) and compound enzymes extraction (cellulase:pectinase:papain). Their characteristics such as the polysaccharide yield, polysaccharide content, protein content, infrared spectra were determined, and antioxidant activities were investigated on the basis of hydroxyl radical, DPPH free radical, ABTS free radical and reducing power. The results showed that five extracts exhibited antioxidant activities in a concentration-dependent manner.

Response surface optimization of enzyme-assisted extraction polysaccharides from Dictyophora indusiata.

An enzyme-assisted procedure for the extraction of the water-soluble polysaccharides from the stipe of Dictyophora indusiata was investigated using response surface methodology. The orthogonal array design was employed to optimize the concentration of three kinds of enzyme (cellulase, papain and pectolyase) and the optimal cellulose, papain and pectolyase concentration were 2.0% (wt.