Publications by authors named "Guilherme Nardi Becker"
Article Synopsis
- Brazil has experienced high COVID-19 cases and deaths, making Latin America a major pandemic epicenter, with early sustained virus transmission and ongoing gaps in understanding.
- Researchers analyzed over 17,000 near-complete SARS-CoV-2 genomes from across Brazil and Paraguay, finding multiple viral lineages predominantly imported from Europe before local transmission clusters emerged after November 2020.
- Effective genomic surveillance has been crucial in tracking the real-time spread of significant variants, such as Gamma (P.1) and Zeta (P.2), and understanding cross-border virus movement into Paraguay.
Article Synopsis
- - Brazil became a major COVID-19 epicenter in Latin America by May 2021, experiencing high transmission rates and deaths, but gaps remain in understanding how the virus spreads nationally.
- - The study analyzes nearly complete SARS-CoV-2 genomes from Brazil and Paraguay, revealing that the initial wave was marked by multiple imported viral lineages mainly from Europe, leading to significant local transmission clusters.
- - As the pandemic progressed without effective restrictions, Brazil saw the emergence and spread of concerning variants like Gamma and Zeta, highlighting the need for extensive genomic surveillance in South America for better pandemic management and public health strategies.
Background: Clinical diagnosis of human brucellosis (HB) is often difficult due to non-specific symptoms. Immunological tests have been the most common method used in HB diagnosis, but molecular methods based on quantitative polymerase chain reaction (qPCR) have largely replaced these diagnostic methods. The aim of this study was to validate a HB diagnostic qPCR method; assessing different target Brucella genes, and the influence of biological matrices (serum vs.
View Article and Find Full Text PDFLaboratory exposures to spp. are preventable. After an outbreak in Brazil, human brucellosis was made statutorily reportable as well as laboratory accidents.
View Article and Find Full Text PDFRecently it was developed the Colistin Broth Disk Elution test which uses colistin disks as a source of these antibiotics. The aim of this study was to evaluate the performance of protocols that used diminished volumes of the reagents: the Colistin Broth Microelution (CBM) (1 mL) and the Microelution-Plates Test (MPT) (200 μL), as well as the Colistin Susceptibility Test Tube (CSTT), which uses only one colistin disk added to a tube containing broth. The tests were performed with 85 Gram-negative isolates collected from surveillance studies.
View Article and Find Full Text PDFBackground: Enterobacter is a common nosocomial microorganism and its carbapenem's resistance has increased. The management of these cases is unclear.
Objective: We evaluated 16 patients with KPC-producing Enterobacter aerogenes infections, detailing the site of infection, therapy, clinical and epidemiological data.