Sulfated chitosan coated polylactide membrane enhanced osteogenic and vascularization differentiation in MC3T3-E1s and HUVECs co-cultures system.

This study aimed to compare the effects of the two type chitosan derivatives, sulfated chitosan (SCS) and phosphorylated chitosan (PCS), coated on poly(d,l-lactide) (PDLLA) membrane via polydopamine, respectively, on vascularization and osteogenesis in vitro. Mouse preosteoblast cells (MC3T3-E1s) and human umbilical vein endothelial cells (HUVECs) were used as co-cultures system. The effects of two type membranes on calcium deposition, alkaline phosphatase (ALP) activity, vascularization related factors nitric oxide (NO) and angiogenic growth factor vascular endothelial growth factor (VEGF) were assessed.

Different influence of sulfated chitosan with different sulfonic acid group sites on HUVECs behaviors.

The vascularization within the scaffold is still a significant challenge in tissue engineering applications. Sulfated chitosan (SCS) as an amazing substance have been used in tissue engineering to stimulate angiogenesis. However, it is not clear whether they have difference in the ability to promote vascularization of SCS with different sulfonic acid group sites.

Sulfonated chitosan and phosphorylated chitosan coated polylactide membrane by polydopamine-assisting for the growth and osteogenic differentiation of MC3T3-E1s.

In this study, a poly(d,l-lactide) (PDLLA) membrane was prepared by the solution casting method, then the surface of the membrane was modified by polydopamine (PDOPA) as a substrate, followed by adsorption of different chitosan derivative sulfonated chitosan (SCS) or/and phosphorylated chitosan (PCS) to obtain different functionalized membranes, and two kinds of chitosan derivatives characterized by FTIR, elemental analysis and zeta potential. And different membranes were evaluated through surface potential, hydrophilicity, surface morphology and chemical compositions. In vitro, the cell culture results showed that the membrane functionalized by chitosan derivative could promote the proliferation of MC3T3-E1s and enhance the osteogenic differentiation by up-regulating the expression level of osteogenic genes compared to the PDLLA and P/PDOPA membranes.