Bosentan, an endothelin receptor antagonist, reduces leucocyte adhesion and inflammation in a murine model of inflammatory bowel disease.

Background And Aims: Endothelins, a group of polyfunctional cytokines, induce the adhesion of circulating leucocytes to venous endothelium, an initial step in the pathogenesis of a cellular infiltrate in inflammatory bowel disease (IBD). The effect of bosentan, a non-selective endothelin receptor antagonist, on leucocyte adhesion and inflammation in a murine model of IBD was studied.

Materials And Methods: Thirty BALB/c mice were divided into three groups of 10 animals: untreated controls, chronic colitis [dextran sodium sulphate (DSS), 3% w/v for 30 days], and treatment with bosentan (30 mg/kg i.

Submucosal collecting venules: a reliable site for intestinal intravital microscopy in rats.

Under physiological conditions and after interventions such as ischemia/reperfusion, postcapillary mesenteric venules are most commonly used for in vivo assessment of intestinal microcirculation by intravital microscopy (IVM). In experimental models of gut inflammation, however, IVM was found to be hampered by postinflammatory tissue injury. In this study, postcapillary submucosal collecting venules located at the junction of mesentery and ileum are introduced and evaluated for IVM in a rat model of indomethacin-induced ileitis.

Immunohistochemical expression of P53 and oncogenes in ulcerative colitis-associated colorectal carcinoma.

The role of the tumor suppressor gene p53 and proto-oncogenes mdm-2, waf-1,and bcl-2 in sporadic colorectal carcinoma (CRC) has been well investigated. However, little is known about the role of these genes in the development of ulcerative colitis-associated colorectal carcinoma (CAC). Colectomy specimens from patients with CAC, patients with ulcerative colitis (UC) and dysplasia, patients with long-standing UC without carcinoma or dysplasia, and patients with CRC were investigated in comparison to normal colon (NC) specimens from patients with diverticulosis without histologic signs of inflammation.