Association of T-cell reactivity with beta-cell function in recent onset type 1 diabetes patients.

Objective: The aim of the current study was to investigate whether autoantigen directed T-cell reactivity relates to beta-cell function during the first 78 weeks after diagnosis of type 1 diabetes.

Research Design And Methods: 50 adults and 49 children (mean age 27.3 and 10.

Effect of heat shock protein peptide DiaPep277 on beta-cell function in paediatric and adult patients with recent-onset diabetes mellitus type 1: two prospective, randomized, double-blind phase II trials.

Background: Aim of this trial was to test whether heat shock protein peptide DiaPep277 treatment in adult and paediatric patients with recent-onset type 1 diabetes (T1D) is safe and whether it can preserve endogenous insulin production.

Methods: Two studies were performed in a prospective, multicentre, double-blind, placebo-controlled trial. Fifty adult (study p520, aged 16-44 years) and 49 paediatric patients (study p521, 4-15 years) with recent-onset T1D were treated subcutaneously at four different time points with 0.

Comparison of cytokine ELISpot assay formats for the detection of islet antigen autoreactive T cells. Report of the third immunology of diabetes society T-cell workshop.

The identification of sensitive assay formats capable of distinguishing islet autoreactive T cells directly ex vivo in blood is a major goal in type 1 diabetes research. Recently, much interest has been shown in the cytokine enzyme linked immunospot assay (CK ELISpot), an assay potentially capable of fulfilling these difficult criteria. To address the utility of this assay in detecting autoreactive T cells, a 'wet' workshop was organized using the same fresh blood sample and coded antigens.

Human heat shock protein 60 induces maturation of dendritic cells versus a Th1-promoting phenotype.

Heat shock protein (HSP) 60 nonspecifically activates cells of the innate immune system. In the present study, we characterized the effects of human HSP60 maturation, cytokine release, and T cell-activating capacity of bone marrow-derived dendritic cells (DC). Furthermore, we analyzed HSP60-induced signal transduction in DC.

Cytokine detection by ELISPOT: relevance for immunological studies in type 1 diabetes.

Diabetes mellitus type 1 is a chronic disease in which the insulin-secreting ss-cells are selectively destroyed by an immune-mediated process. Autoantibodies directed against several islet antigens are useful parameters to estimate the risk to develop diabetes, but cell-mediated immunity involving T lymphocytes plays a major part in causing the specific destruction of ss-cells. T cells are characterized by their antigen-specificity, phenotype and cytokine-secreting profile.