Insights into Emydid Turtle Cytogenetics: The European Pond Turtle as a Model Species.

Our knowledge of Testudines evolution is limited by the lack of modern cytogenetic data. Compared to other reptiles, there is little information even on chromosome banding, let alone molecular cytogenetic data. Here, we provide detailed information on the karyotype of the European pond turtle Emys orbicularis, a model Emydidae, employing both chromosome banding and molecular cytogenetics.

The Weierstrassian movement patterns of snails.

Weierstrassian Lévy walks are the archetypical form of random walk that do not satisfy the central limit theorem and are instead characterized by scale invariance. They were originally regarded as a mathematical abstraction but subsequent theoretical studies showed that they can, in principle, at least, be generated by chaos. Recently, Weierstrassian Lévy walks have been found to provide accurate representations of the movement patterns of mussels () and mud snails () recorded in the laboratory under controlled conditions.

Serpentine soils affect heavy metal tolerance but not genetic diversity in a common Mediterranean ant.

Natural habitats with serpentine soils are rich in heavy metal ions, which may significantly affect ecological communities. Exposure to metal pollutants results, for instance, in a reduction of population genetic diversity and a diffused higher tolerance towards heavy metals. In this study, we investigated whether chronic exposure to metals in serpentine soils affect accumulation patterns, tolerance towards metal pollutants, and genetic diversity in ants.

The Rules of Aggression: How Genetic, Chemical and Spatial Factors Affect Intercolony Fights in a Dominant Species, the Mediterranean Acrobat Ant Crematogaster scutellaris.

Nest-mate recognition plays a key role in the biology of ants. Although individuals coming from a foreign nest are, in most cases, promptly rejected, the degree of aggressiveness towards non nest-mates may be highly variable among species and relies on genetic, chemical and environmental factors. We analyzed intraspecific relationships among neighboring colonies of the dominant Mediterranean acrobat ant Crematogaster scutellaris integrating genetic, chemical and behavioral analyses.

Pre-exposure to Cadmium or Zinc Alters the Heart Rate Response of the Crayfish Procambarus clarkii Towards Copper.

Exposure to sub-lethal concentrations of a pollutant induces, in some organisms, an acclimation process which increases their resistance to other substances (cross-acclimation). Understanding this phenomenon is important as a basis for a better comprehension of the effects of pollutants in ecosystems. In this paper we investigated whether the exposure to Cd or Zn is able to modify the heart rate response of the crayfish Procambarus clarkii to acute Cu stress.

When the rule becomes the exception. no evidence of gene flow between two Zerynthia cryptic butterflies suggests the emergence of a new model group.

There is increasing evidence that most parapatric cryptic/sister taxa are reproductively compatible across their areas of contact. Consequently, the biological species concept, which assumes absence of interbreeding, is becoming a not so effective criterion in evolutionary ecology. Nevertheless, the few parapatric sister taxa showing complete reproductive barriers represent interesting models to study speciation processes and the evolution of reproductive isolation.

Exploring the effects of seasonality and chemical pollution on the hepatopancreas transcriptome of the Manila clam.

The assessment of marine environmental health is a complex but fundamental task both for ecosystem conservation and food safety related to the human consumption of marine products. Manila clams inhabiting the Venice Lagoon constitute an excellent case study for evaluating the effects of complex mixtures of industrial and urban effluents on aquatic organisms. Clams were collected in different seasons at four locations within the Venice Lagoon.

Transcriptome sequencing and microarray development for the Manila clam, Ruditapes philippinarum: genomic tools for environmental monitoring.

Background: The Manila clam, Ruditapes philippinarum, is one of the major aquaculture species in the world and a potential sentinel organism for monitoring the status of marine ecosystems. However, genomic resources for R. philippinarum are still extremely limited.

Inhibitory effect of Pb2+ on the transport cycle of the Na+,K+-ATPase.

The effect of Pb(2+) on the transport cycle of the Na(+),K(+)-ATPase was characterized in detail at a molecular level by combining electrical and biochemical measurements. Electrical measurements were performed by adsorbing purified membrane fragments containing Na(+),K(+)-ATPase on a solid-supported membrane. Upon adsorption, the Na(+),K(+)-ATPase was activated by carrying out concentration jumps of different activating substrates, for example, Na(+) and ATP.

Characterization of microsatellite loci in the European pond turtle Emys orbicularis.

A set of eight highly polymorphic microsatellite markers was isolated and characterized from a genomic library enriched for dinucleotide repeats in the European pond turtle, Emys orbicularis. The markers were tested for polymorphism in a total of 33 turtles sampled in two natural ponds in the nature reserve of Kerkini, northern Greece. Number of alleles varied from 10 to 18, and expected heterozygosity ranged between 0.

Acclimatable cardiac and ventilatory responses to copper in the freshwater crayfish Procambarus clarkii.

Mortality and physiological tests following exposure to waterborne copper were performed in the red swamp crayfish Procambarus clarkii from a central Italian population. Mortality tests gave an estimated 96 h LC50 value (with 95% confidence limits) of 162 (132-211) mg L(-1) waterborne copper II. Variations in cardiac and ventilatory rates were simultaneously monitored using a non-invasive plethysmographic technique.

Neuronal control of the cardiac responses to osmotic stress in the gastropod limpet Patella caerulea.

Mediterranean limpets Patella caerulea were exposed to different salinity conditions and treated with drugs interfering with neuronal control of heartbeat. Heart rate was monitored using a non-invasive method. Limpets were superfused with control (33 g l(-1)), hyposaline (0 and 10 g l(-1)) or hypersaline (56 and 66 g l(-1)) artificial seawater.

Tetrodotoxin prevents copper-induced bradycardia in gastropod limpets.

Acute exposure to waterborne copper is followed by a reduction in heart rate in gastropod limpets. In order to understand the mechanism of this effect, exposure to copper (0.25 or 0.

Thermal benefits from familiarity with the environment in a reptile.

The tortoise Testudo hermanni Gmelin is non-territorial, frequents non-personal shelters and is generalist in diet, but stays within a home range very stable in time. Analysis of shell temperatures obtained at set intervals by radiothermometers showed that while tortoises resident in the study area behave as homoiotherms, animals imported from a similar environment appear relatively poikilotherms. Moreover, residents are more efficient in basking, reaching the daily thermal maxima systematically earlier than the unfamiliar animals.