Dual-potential electrochemiluminescence cytosensor based on a metal-organic framework and ABEI-PEI-Au@AgNPs for the simultaneous determination of phosphatidylserine and epidermal growth factor receptors on an apoptotic cell surface.

A new electrochemiluminescence (ECL) cytosensor is proposed for the simultaneous determination of phosphatidylserine (PS) and epidermal growth factor receptor (EGFR) based on the ECL signals of metal-organic framework-5 (MOF-5) loaded CdS quantum dots and N-(aminobutyl)-N-(ethylisoluminol)-polyethylenimine capped Au and Ag nanoparticles. Apoptosis promotes the exposure of PS and reduces the expression of EGFR in cell membranes. Two spatially resolved areas on dual-disk glassy carbon electrodes were designed to eliminate the interference from different ECL probes.

Electrochemiluminescence resonance energy transfer of MnCO for ultrasensitive amyloid-β protein detection.

A composite material MnCO/poly(diallyl dimethyl ammonium chloride) (PDDA)/Ag with excellent electrochemiluminescence (ECL) performance and high biocompatibility was prepared by adding MnCO and PDDA to silver nanoparticles (AgNPs). MnCO/PDDA/Ag and Au@SiONPs were used as ECL donors and acceptors, respectively. Thus, an effective ECL-resonance energy transfer (RET) sensing platform was established.

[Selenium speciation in watermelon by g-CN enrichment combined with capillary electrophoresis-inductively coupled plasma-mass spectrometry].

Selenium is one of the essential trace elements in the human body, and it plays a critical role in human health. In this work, 2.0 g melamine was placed in an alumina crucible, which was heated in a box-type resistance furnace for 2 h at 600 ℃, at the heating rate of 3 ℃/min, and then cooled to room temperature.

Spatially-resolved dual-potential sandwich electrochemiluminescence immunosensor for the simultaneous determination of carbohydrate antigen 19-9 and carbohydrate antigen 24-2.

A new electrochemiluminescence (ECL) immunosensor based on spatially-resolved dual-potential technology was designed for the simultaneous determination of carbohydrate antigen 19-9 (CA 19-9) and carbohydrate antigen 24-2 (CA 242). Luminol-AgNPs@ZIF-67 was used as the anodic probe, and Pt nanoparticle-functionalized graphitic carbon nitride nanosheets (g-CN@PtNPs) were used as the cathodic probe. Two spatially-resolved areas on the dual-disk glassy carbon electrode (DDGCE) were modified with a AuNPs film by electrodeposition to improve the conductivity of the sensing interface.

A potential-resolved electrochemiluminescence resonance energy transfer strategy for the simultaneous detection of neuron-specific enolase and the cytokeratin 19 fragment.

An electrochemiluminescence resonance energy transfer (ECL-RET) immunosensor was developed based on the potential-resolved technology for the simultaneous detection of neuron-specific enolase (NSE) and the cytokeratin 19 fragment (CYFRA21-1). The absorption spectrum of gold nanorods (AuNRs) perfectly overlapped with the ECL spectra of SnS2@Pt and Ru(bpy)32+/Zn-MOF, so they exhibited an excellent ECL-RET effect with high efficiency. Zn-MOF possesses a large surface area, which allows for the loading of Ru(bpy)32+.

Electrochemiluminescence immunoassay of human chorionic gonadotropin using silver carbon quantum dots and functionalized polymer nanospheres.

A composite, reduced graphene oxide (rGO) doped with silver nanoparticles (Ag NPs), was prepared by using binary reductants of sodium citrate and hydrazine hydrate. Carbon quantum dots (CQDs) synthesized by papaya peel combined with silver ions to form a CQDs-loaded silver nanoparticle (AgCQDs) nanocomposite. Polymer nanospheres (PNS) were generated via the infinite coordination polymer of ferrocene dicarboxylic acid and employed as carriers to load AgCQDs.

Electrochemiluminescent immunoassay for neuron specific enolase by using amino-modified reduced graphene oxide loaded with N-doped carbon quantum dots.

An ultrasensitive electrochemiluminescence based sandwich immunoassay is presented for determination of neuron specific enolase. The method uses silver-cysteine nanowires as the capture probe and a composite made of amino-modified reduced graphene oxide and nitrogen-doped carbon quantum dots as the signal probe. It was synthesized by covalent coupling of amino-modified reduced graphene oxide to the carboxy groups of nitrogen-doped carbon quantum dots.

A highly efficient introduction system for single cell- ICP-MS and its application to detection of copper in single human red blood cells.

A method of simultaneous cell counting and determination of metals in single cells using time-resolved inductively coupled plasma-mass spectrometry (ICP-MS) was reported. A facile, low cost and highly efficient single-cell introduction system of time-resolved ICP-MS consists of a flow cell, a visual contrast calibration device, a customized nebulizer and a fabricated spray chamber. The flow cell includes a cell sample tube, a sheath liquid tube and a flow chamber.

Facile Preparation of Boron and Nitrogen Codoped Green Emission Carbon Quantum Dots for Detection of Permanganate and Captopril.

A hydrothermal strategy for preparing boron and nitrogen codoped carbon quantum dots was studied using the precursors of -amino salicylic acid, boric acid and ethylene glycol dimethacrylate. The boron and nitrogen codoped carbon quantum dots have high fluorescence intensity, good monodispersity, high stability, superior water solubility, and a fluorescence quantum yield of 19.6%.

A Novel Carbon Quantum Dots Signal Amplification Strategy Coupled with Sandwich Electrochemiluminescence Immunosensor for the Detection of CA15-3 in Human Serum.

A sensitive sandwich electrochemiluminescence immunosensor was established by employing graphene oxide-PEI-carbon quantum dots (CQDs)-Au nanohybrid as probe to measure carbohydrate antigen 15-3 (CA15-3), a breast cancer biomarker. In this work, nanocomposites of Ag nanoparticles and polydopamine (AgNPs-PDA) were synthesized by redox reaction between dopamine and Ag. The nanocomposite with high surface area can provide an efficient substrate for immobilizing initial antibody (Ab1).

A novel ECL sensor based on a boronate affinity molecular imprinting technique and functionalized SiO@CQDs/AuNPs/MPBA nanocomposites for sensitive determination of alpha-fetoprotein.

In this work, a boronate-affinity sandwich electrochemiluminescence (ECL) sensor was constructed to detect alpha-fetoprotein (AFP) based on a multiple signal amplification strategy. Gold nanoparticles (AuNPs) were utilized and modified on the surface with chitosan in order to facilitate electron transfer. The composite of the molecularly imprinted polymer (MIP) enhanced the selectivity of alpha-fetoprotein detection.

Ginkgo leaf-based synthesis of nitrogen-doped carbon quantum dots for highly sensitive detection of salazosulfapyridine in mouse plasma.

A simple, economical hydrothermal strategy for synthesizing nitrogen-doped carbon quantum dots (N-CQDs) was developed using Ginko leaves as a carbon source. These N-CQDs have strong blue fluorescence, excitation-relevant emissions, high monodispersity, good stability, good water solubility, and a 22.8% fluorescence quantum yield.

Based on reduced graphene oxide-copper sulfide-carbon nitride nanosheets composite electrochemiluminescence sensor for determination of gatifloxacin in mouse plasma.

A new method for determination of gatifloxacin hydrochloride (GAT) by reduced graphene oxide-copper sulfide (rGO-CuS) composite coupled with graphite-like carbon nitride nanosheets (g-CN NSs) modified glassy carbon electrode was developed. In this work, rGO-CuS composite was synthesized by one-pot hydrothermal method and used for enhancing sensitivity of GAT. g-CN NSs were synthesized as radiant agent.

Based on ZnSe quantum dots labeling and single particle mode ICP-MS coupled with sandwich magnetic immunoassay for the detection of carcinoembryonic antigen in human serum.

A highly sensitive sandwich-type magnetic immunoassay based on inductively coupled plasma mass spectrometry detection in single particle mode, with ZnSe Quantum dots (QDs) serving as model tags, was proposed. The transient signals induced by the flash of ions (Zn) in the plasma torch from the ionization of nanoparticles tagged on antibody were recorded in a single particle mode. The frequency of transient signals is directly related to the concentration of nanoparticle tags, and the concentration of nanoparticle tagged antibodies can be quantified by the frequency of transient signals.