[Determining of the content of determination of elements of Pedicularis from different places by ICP-MS after micriware-assisted digestion].

The content of elements in Pedicularis was determined by ICP-MS aftermicriware-assisted digestion. Recovery and RSD of the method is 95%-102% and 1.24%-2.

Preparative separation of methylswertianin, swerchirin and decussatin from the Tibetan medicinal plant Swertia mussotii using high-speed counter-current chromatography.

Introduction: Xanthones, the primary constituents of Swertia mussotii, are known to possess a variety of biological activities, including anti-depressant, anti-leukaemic, anti-tumour, anti-tubercular, choleretic, diuretic, anti-microbial, anti-fungal, anti-inflammatory, anti-viral, cardiotonic and hypoglycemic properties. However, high performance, environmentally friendly methods for isolating and purifying xanthones from S. mussotii are not currently available.

[Determination of trace determination of elements in medicinal materials of cultivated and wild Rhizoma et Radix Notopterygii vegetated in different months by flame atomic absorption spectrometry].

The contents of six trace elements Ca, Mg, Fe, Mn, Cu and Zn in the medicinal materials of cultivated and wild Rhizoma et Radix Notopterygii vegetated in different months were determined by flame atomic absorption spectrometry (FAAS) with air-acetylene flame, and the feature of trace elements contents and their seasonal dynamics were analyzed using SPSS16.0 analysis software. The recovery rate obtained by standard addition method ranged between 96.

Comparative study on mineral elements in the roots of Rheum tanguticum from Qinghai-Plateau.

In the present paper, the authors analysed 10 mineral elements in the roots of Rheum tanguticum collected from 30 different habitats. The mean concentration values of the 10 elements decreased as follows: Ca>Mg>K>Fe>Mn>Cr>Zn>Ni>Cu>Se. Ca, Mg, K and Fe were abundant in this herb.

Glucose-6-phosphate dehydrogenase-dependent hydrogen peroxide production is involved in the regulation of plasma membrane H+-ATPase and Na+/H+ antiporter protein in salt-stressed callus from Carex moorcroftii.

Glucose-6-phosphate dehydrogenase (G6PDH) is important for the activation of plant resistance to environmental stresses, and ion homeostasis is the physiological foundation for living cells. In this study, we investigated G6PDH roles in modulating ion homeostasis under salt stress in Carex moorcroftii callus. G6PDH activity increased to its maximum in 100 mM NaCl treatment and decreased with further increased NaCl concentrations.

[Season features of mineral elements in the root of cultivated and wild Anisodus tanguticus].

The Anisodus tanguticus (Maxim.) Pascher is a Chinese traditional medicinal material and Tibet herb of local Qing-hai. The authors collected, tanguticus and wild Anisodus tanguticus (Maxim.

Effects of emodin on the gene expression profiling of human breast carcinoma cells.

Background: The mechanism of emodin-mediated cell apoptosis has been investigated extensively in many types of human cancer cells. Our previous study demonstrated that emodin induced apoptosis through the decrease of Bcl-2/Bax ratio and the increase of cytoplasm cytochrome c concentration in human breast cancer BCap-37 cells. However, emodin's reaction to breast cancer cells remains elusive.

Proteomic detection of changes in protein expression induced by cordycepin in human hepatocellular carcinoma BEL-7402 cells.

The nucleoside analogue cordycepin (3 '-deoxyadenosine, 3 '-dA), one of the components of Cordyceps militaris, has been shown to inhibit the growth of various tumor cells. However, the probable mechanism is still obscure. In this study, the inhibition of cell growth and changes in protein expression induced by cordycepin were investigated in BEL-7402 cells.

Rhein induces apoptosis and cell cycle arrest in human hepatocellular carcinoma BEL-7402 cells.

Rhein, an anthraquinone derivative of rhubarb, inhibits the proliferation of various human cancer cells. In this paper, we focused on studying the effects of rhein on human hepatocelluar carcinoma BEL-7402 cells and further understanding the underlying molecular mechanism in an effort to make the potential development of rhein in the treatment of cancers. Using MTT assay and flow cytometry, we demonstrate a critical role of rhein in the suppression of BEL-7402 cell proliferation in a concentration- and time-dependent manner.

Emodin-induced apoptosis in human breast cancer BCap-37 cells through the mitochondrial signaling pathway.

Emodin, a natural anthraquinone compound isolated from the rhizome of rhubarb, is reported to suppress the growth of tumor in many clinical situations. In this study, we focused on the effect of emodin in human breast cancer BCap-37 cells and further understand the underlying molecular mechanism in treating breast cancer. Using MTT assay and flow cytometry, we demonstrated the critical role of emodin in the suppression of the proliferation of BCap-37 cells based on a concentration-and time-dependent manner.

Effects of azadirachtin on six inorganic cation distributions in Ostrinia furnacalis (G.).

Azadirachtin (Az), as a botanical insecticide, is relatively safe and biodegradable. It affects a wide variety of biological processes, including the reduction of feeding, suspension of molting, death of larvae and pupae, and sterility of emerged adults in a dose-dependent manner. However,the mode of action of this toxin remains obscure.

Determination of long-chain fatty acids in bryophyte plants extracts by HPLC with fluorescence detection and identification with MS.

A sensitive method for the determination of long-chain fatty acids (LCFAs) (>C20) using 1-[2-(p-toluenesulfonate)-ethyl]-2-phenylimidazole-[4,5-f]-9,10-phenanthrene (TSPP) as tagging reagent with fluorescence detection and identification with post-column APCI/MS has been developed. The LCFAs in bryophyte plant samples were obtained based on distillation extraction with 1:1 (v/v) chloroform/methanol as extracting solvent. TSPP could easily and quickly label LCFAs at 90 degrees C in the presence of K2CO3 catalyst in DMF.

Influence of lanthanum on the accumulation of trace elements in chloroplasts of cucumber seedling leaves.

Influence of La3+ on the accumulation of trace elements (75Se, 56 Co, 83 Rb, 48 V, 95 mTc, and 67Ga) in chloroplasts of cucumber seedling leaves was studied by a radioactive multi-tracer technique. At the same time, chloroplast contents of different concentrations of La3+ treatment were calculated. It was observed that chloroplast contents peaked at 0.

[Study on HPLC fingerprint of Swertia mussotii var. mussotii from north-west area of Sichuan Province I].

Objective: To constitute HPLC fingerprint of the methanol extract from Swertia mussotii grown in Sichuan Province.

Method: RP-HPLC, methanol and water including 0.02% acid as mobile phase, gradient elution, flow rate 1.

[Interrelation between plant species diversity and soil environmental factors in bird island of Qinghai Lake].

The study on the relationship between plant species diversity and soil factors in the bird island of Qinghai Lake indicated that this island was a low diversity district, its Shannon-Wienner index and species richness decreased with the increasing soil available K, water soluble salt concentration and pH, and there were significant linear and quadratic correlations between them. Stepwise linear regressions showed that soil available K and water soluble salt were the key factors to estimate Shannon-Wienner index and species richness in this island, respectively, and no correlation was found between species evenness and soil factors.

[Determination of haleniaside and demethoxyhaleniaside in wild and cultivated Halenia ellipitica D. Don. by reversed-phase high performance liquid chromatography].

A simple and accurate method for the determination of haleniaside and demethoxyhaleniaside in Halenia ellipitica D. Don. materials and patent medicines with high performance liquid chromatography (HPLC) was developed.

Influence of lanthanum on the uptake of trace elements in cucumber plant.

The effects of La3+ on the uptake of trace elements (Se, Co, V, and Tc) in cucumber plants were studied by a radioactive multitracer technique. It was observed that the uptake and distribution of these trace elements in roots, stems, and leaves are different under different La3+ treatments. Furthermore, in the control, the plant accumulates 75Se, 56Co, and 48V all in the order roots>leaves>stems, whereas 95mTc was in the order leaves>stems>roots.