Clinical application of chromosome microarray analysis and karyotyping in prenatal diagnosis in Northwest China.

Introduction: Karyotyping and chromosome microarray analysis (CMA) are the two main prenatal diagnostic techniques currently used for genetic testing. We aimed to evaluate the value of chromosomal karyotyping and CMA for different prenatal indications.

Methods: A total of 2084 amniocentesis samples from pregnant women who underwent prenatal diagnosis from 16 to 22 + 6 weeks of gestation between January 2021 and December 2022 were retrospectively collected.

Triglyceride-glucose index in early pregnancy predicts the risk of gestational diabetes: a prospective cohort study.

Objective: This study aimed to investigate the association between the triglyceride-glucose (TyG) index in early pregnancy and the development of gestational diabetes mellitus (GDM) in the second trimester. The primary objectives were to evaluate the predictive potential of the TyG index for GDM, determine the optimal threshold value of the TyG index for GDM assessment, and compare the predictive performance of the TyG index alone versus its combination with maternal age and pre-pregnancy body mass index on GDM. Moreover, the study explored the association between the TyG index in early pregnancy and the risk of other pregnancy-related complications (PRCs), such as placental abruption and gestational hypertension.

Clinical application of single nucleotide polymorphism microarray analysis in pregnancy loss in Northwest China.

Spontaneous abortion is the most common complication of early pregnancy. In this study, we aim to investigate the clinical application value of genetic diagnosis using single nucleotide polymorphism (SNP) microarray analysis on the products of conception and to characterize the types of genetic abnormalities and their prevalence in pregnancy loss in Northwest China. Over 48 months, we selected 652 products of conception, which included chorionic villi, fetal tissues, germ cell samples, amniotic fluid samples, cord blood samples, and a cardiac blood sample.

Association Analysis of Gene Sequencing by NeoSeq Combined with Tandem Mass Spectrum and Four Neonatal Diseases.

Background: NeoSeq is a new method of gene sequencing for newborn screening. The goal is to explore the relationship between gene sequencing by NeoSeq combined with tandem mass spectrum (TMS) and four neonatal diseases.

Methods: A total of 1,989 newborns from August 2010 to December 2021 were enrolled.

Integrated Proteomic and N-Glycoproteomic Profiling of Placental Tissues of Patients with Preeclampsia.

Background: Preeclampsia (PE) is a multi-system disorder of pregnancy that poses a serious threat to maternal and perinatal health worldwide. This study aims to evaluate the global alterations of protein expression and N-glycosylations that are crucial for PE pathogenesis. Here, tandem mass tag labeling combined with LC-MS/MS was employed to determine the global expression of all proteins and intact glycopeptide in placentas from three healthy pregnant women, three patients with early-onset severe PE, and three patients with late-onset severe PE.

From an identity process theory perspective: a daily investigation of why and when ostracism triggers ingratiation.

Although existing studies suggest the relationship between ostracism and ingratiation, the knowledge about why and when ostracism promotes ingratiatory behaviors remains limited. Drawing from identity process theory, the current study examines the influence of ostracism on ingratiatory behaviors through the mediating role of self-identity threat on a daily timescale and the cross-level moderation of core self-evaluation. Through a diary study of 117 Chinese college students across 14 consecutive days, we found that daily ostracism had a positive indirect effect on daily ingratiatory behaviors through daily self-identity threat.

Associations between AGT, MTHFR, and VEGF gene polymorphisms and preeclampsia in the Chinese population.

Introduction: Preeclampsia (PE) is a pregnancy-specific multisystemic syndrome. This study aimed to investigate the associations between angiotensinogen (AGT), methylenetetrahydrofolate reductase (MTHFR), vascular endothelial growth factor (VEGF) polymorphisms, and PE in the Han Chinese population.

Methods: We genotyped 26 single-nucleotide polymorphisms (SNP) in three genes by using QuantStudio™ 12 K Flex Real-Time PCR technology in 168 patients with PE and 204 healthy pregnant control subjects.

Screening and Functional Analysis of Gene Mutations in a Cohort of Chinese Patients With Congenital Hypothyroidism.

Backgrounds: As a crucial enzyme in thyroid hormone synthesis, the genetic defective thyroid peroxidase () was one of the main genetic factors leading to congenital hypothyroidism (CH).

Methods: Mutations in the gene were screened and identified in 219 patients with CH from northwest China by using high-throughput sequencing and bioinformatics analysis. The biological function of detected variants was studied by experiments and homology modeling.

Ethnic disparities in postpartum hemorrhage after cesarean delivery: a retrospective case-control study.

Purpose: To explore the relationship of ethnicity and postpartum hemorrhage (PPH) for women who underwent cesarean delivery (CD) and examine the risk factors for PPH in distinct ethnic groups in China.

Methods: We conducted case-control studies with the maternity data from the 11,778 CD cases, in Xinjiang Uygur Autonomous Region. Initially, multivariable logistic regression was used to estimate the disparity of race-ethnicity on the risk of PPH in ethnic Han, Uygur, Hui and Kazakh.

Conservative management versus cesarean hysterectomy in patients with placenta increta or percreta.

Objective: To compare conservative management and cesarean hysterectomy in patients with placenta increta or percreta.

Materials And Methods: In this multicenter retrospective study, we recorded data on 2219 patients with placenta increta or percreta from 20 tertiary care centers in China from 1 January 2011 to 31 December 2015. Propensity score analysis was used to control for baseline characteristics.

The prevalence of CGG repeat expansion mutation in FMR1 gene in the northern Chinese women of reproductive age.

Background: The prevalence of CGG repeat expansion mutation in FMR1 gene varies among different populations. In this study, we investigated the prevalence of this mutation in women of reproductive age from northern China.

Methods: A total of 11,891 pre-conceptional or pregnant women, including 5037 pregnant women and 7357 women with the history of spontaneous abortion or induced abortion due to delayed growth of the embryos, were recruited.

Efficacy of tenofovir in preventing perinatal transmission of HBV infection in pregnant women with high viral loads.

Mother-to-child transmission is the major cause of chronic hepatitis B virus (HBV) infection. This double-blind trial tested the effect of tenofovir disoproxil fumarate (TDF) in preventing vertical transmission. Pregnant women who were HBsAg/HBeAg-positive with a HBV DNA titer ≥ 2×10 IU/mL were randomly assigned to the control (n = 60) and TDF-treated (n = 60) groups.

Mutational Spectrum Analysis of Seven Genes Associated with Thyroid Dyshormonogenesis.

Objective: Thyroid dyshormonogenesis (DH) is a genetically heterogeneous inherited disorder caused by thyroid hormone synthesis abnormalities. This study aims at comprehensively characterizing the mutation spectrum in Chinese patients with DH.

Subjects And Methods: We utilized next-generation sequencing to screen for mutations in seven DH-associated genes (TPO, DUOX2, TG, DUOXA2, SLC26A4, SLC5A5, and IYD) in 21 Chinese Han patients with DH from Xinjiang Province.

Post-marketing safety surveillance and reevaluation of Motherwort injection: A clinical study of 10 094 cases.

Objective: To investigate the safety profiles of Motherwort injection (MI).

Methods: A multi-center, prospective and drug- derived hospital intensive monitoring method was conducted to assess the safety of MI in real world applications. This study was based on a very large population after the injection was approved and marketed in China.

The value of the 24-h proteinuria in evaluating the severity of preeclampsia and predicting its adverse maternal outcomes.

Background: To identify the 24-h proteinuria value with quantitative analysis and how it correlates with the severity of preeclampsia and subsequent adverse maternal outcomes in the Chinese population.

Study Design: Eleven hospitals in 10 provinces across China were chosen, in which 1,738 pregnant women complicated by hypertensive disorders of pregnancy (HDP) with the records of 24 h proteinuria were enrolled. They were allocated into four groups: patients with maximal quantified proteinuria < 0.

Maternal and neonatal outcomes of placenta increta and percreta from a multicenter study in China.

Objective: The objective of this study is to identify the maternal and neonatal outcomes in women with placenta increta or placenta percreta in China.

Materials And Methods: We retrospectively analyzed 2219 cases from 20 tertiary care centers in China between January 2011 and December 2015. All cases were diagnosed of placenta increta or placenta percreta, based on either intraoperative findings or histopathological findings.

A study of the association between the sFlt-1/PIGF ratio and preeclampsia in Xinjiang Uygur Autonomous Region of China.

Aim: It was previously reported that the ratio of soluble fms-like tyrosine kinase-1 (sFlt-1) to placental growth factor (PlGF) can predict the clinical onset of preeclampsia. This study seeks to validate the association between ratios of sFlt-1/PlGF with preeclampsia and to identify the contribution of ethnicity across diverse populations of the Xinjiang Uygur Autonomous Region.

Methods: Pregnant women were classified into those with preeclampsia (n = 136) and healthy controls (n = 350).

A protein in pig spleen similar to immune suppressive protein of stress.

Aim: To purify a protein in pig spleens, which was similar to immune suppressive protein of stress (ISPS), and characterize its properties and functions.

Methods: 1) Pig spleen was extracted in dilute hydrochloric acid. 2) The extract was ultra-filtrated for having high molecular weight proteins (Mr>30 000).

[Involvement of immune system in the pathogenesis of viral myocarditis].

Myocarditis is thought to be commonly caused by various viruses, and accumulating evidence links viral myocarditis with the eventual development of dilated cardiomyopathy. Heart disease is the most prevalent cause of morbidity and mortality in developed countries. Cytokines are being increasingly recognized as an important factor in the pathogenesis of myocarditis and cardiomyopathy.

[Calcitonin gene-related peptide induces chemokine interleukine-8 synthesis in human monocytes].

Objective: To study the effect of calcitonin gene-related peptide (CGRP) on the function of immune cells.

Methods: Human monocytes were cultured with calcitonin gene-related peptide in vitro and activated with lipopolysaccharide (LPS). The IL-8 level in the supernatant was measured with ELISA and the IL-8 mRNA expression in monocytes was observed by reverse transcription polymerase chain reaction (RT-PCR).