Publications by authors named "Gui-zhen Zhao"
Aim: To build a hepatitis B virus (HBV)-infected human trophoblast cell model in vitro and determine the mechanism of intrauterine HBV infection.
Methods: Serum from hepatitis B-infected patients containing HBV DNA >10(9) was drawn, subsequently inoculated into human trophoblast cells in vitro (JEG3) and passage-cultured. The supernatants and intracellular HBV viral load of inoculated cells were tested by real-time PCR, and HBV DNA was determined by Southern blot.
[Study on the relationship between the serum levels of Th1/Th2 cytokines and the clinical manifestations of chronic hepatitis C and the outcome of interferon therapy].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
October 2009
Article Synopsis
- The study aimed to explore how serum levels of Th1/Th2 cytokines relate to the progression of viral hepatitis C and the effectiveness of interferon therapy.
- Researchers used tests like ELISA and quantitative PCR to measure cytokines and HCV loads, finding that cytokine levels differed between chronic hepatitis C patients and healthy controls.
- Results showed that while no cytokine levels correlated with interferon therapy outcomes before treatment, increased serum IFN-gamma after treatment was linked to better long-term responses.
Article Synopsis
- The study investigates how the hepatitis B virus (HBV) is passed from pregnant women to their fetuses, focusing on placental tissue and cultured cells.
- Various laboratory techniques, including ELISA and RT-PCR, were used to examine placental samples from pregnant women with HBV.
- Findings indicated a correlation between HBV levels in mothers and their babies; HBV was present in placental cells, suggesting that the virus can cross the placental barrier and potentially infect the fetus.
[The factors associated with viral relapse after interferon treatment in chronic hepatitis C patients].
Zhonghua Gan Zang Bing Za Zhi
August 2006
Article Synopsis
- The study aimed to explore the links between hepatitis C virus (HCV) genotype, viral load, ALT levels, and factors influencing viral relapse post-IFN treatment in chronic hepatitis C patients.
- The research involved analyzing HCV RNA levels and genotypes in 208 patients treated with PEG-IFN alpha -2a and Roferon-A over a 24-week period, followed by a 24-week monitoring phase.
- Results showed a high viral relapse rate of 48.7% among responders, with higher relapse rates in genotype 1 patients compared to non-genotype 1, suggesting that HCV genotype has a significant impact on the likelihood of relapse after treatment.
[Retreatment with interferon of relapsed chronic hepatitis C patients].
Zhonghua Gan Zang Bing Za Zhi
January 2006
Article Synopsis
- The study aimed to assess the effectiveness of interferon (IFN) retreatment in chronic hepatitis C patients who relapsed after prior IFN therapy, focusing on various influencing factors.
- Significant results indicated that PEG-IFNalpha-2a treatment led to higher end of treatment virus response (ETVR) and sustained viral response (SVR) rates compared to CIFNalpha-2a, particularly in patients with genotype 1.
- Overall, while some patients did not respond to retreatment, PEG-IFNalpha-2a was found to be more effective than CIFNalpha-2a, with viral load having no impact on treatment response.
Article Synopsis
- This study investigates the factors that predict sustained viral response in chronic hepatitis C patients treated with two types of interferon: roferon-A and pegasys.
- The research involved a randomized trial with assessments of HCV genotypes and RNA levels before and after a 24-week treatment, examining various clinical characteristics using logistic regression.
- Results indicated that younger, female patients with specific infection routes and lower viral loads had better response rates, while those with higher AST/ALT ratios and viral loads showed greater sustained response post-treatment.
Article Synopsis
- The study aimed to identify predictors of response to interferon (IFN) therapy in chronic hepatitis C patients using multivariate logistic regression analysis.
- Patients were randomly assigned to receive either pegasys or Roferon-A for 24 weeks, with their HCV RNA levels measured before, during, and after treatment.
- The results indicated that while HCV genotype was not a predictor of immediate viral response, it was significant for sustained response, with pegasys and HCV genotype being key independent factors affecting treatment outcomes.
Impact of virus genotype on interferon treatment of patients with chronic hepatitis C: a multicenter controlled study.
Hepatobiliary Pancreat Dis Int
August 2004
Article Synopsis
- The study evaluated how HCV genotype affects the response to interferon treatment in patients with chronic hepatitis C.
- A total of 202 patients were analyzed, with a majority infected by genotype 1, who showed lower sustained viral response (SVR) rates compared to those with non-1 genotypes.
- Results indicated that patients with genotype 1 had a higher likelihood of viral relapse and lower treatment effectiveness, suggesting that genotype significantly influences treatment success.
[The influence of HCV genotype on the IFN treatment of patients with chronic hepatitis C].
Zhonghua Gan Zang Bing Za Zhi
February 2004
Article Synopsis
- This study examines how different HCV genotypes impact the effectiveness of interferon (IFN) treatment in chronic hepatitis C patients.
- It was found that patients with genotype 1 had lower sustained viral response (SVR) rates (25.3%) compared to those with non-1 genotypes (43.2%), suggesting genotype 1 may lead to less effective treatment outcomes.
- While Pegasys treatment led to comparable end-of-treatment viral response (ETVR) rates for both genotypes, the significant difference in SVR rates highlights the need for personalized treatment approaches based on HCV genotype.
Objective: To study the role of tumor necrosis factor-alpha (TNFalpha) and caspase-3 expression on hepatocyte apoptosis in experimental model of fulminant hepatic failure (FHF).
Methods: Mouse experimental model of FHF was induced by lipopolysaccharide (LPS) and D-galactosamine (D-GalN). Serum TNFalpha level and TNFalpha mRNA expression in liver were tested by ELISA and reverse transcriptase PCR (RT-PCR) method, respectively.
HCV genotypes in hepatitis C patients and their clinical significances.
World J Gastroenterol
December 1999