Objective: To determine the content of total flavonoids and rutin in different parts of wild and cultivated Hypericum attenuatum.
Methods: Ethanol ultrasonic extraction method was used to extract total flavonoids and rutin in different parts of Hypericum attenuatum. With rutin as reference substance, UV spectrophotometry and high performance liquid chromatography were applied to deter- mine the total flavonoids and rutin content in Hypericum attenuatum samples (leaves, stems and flowers).