Intestinal Absorption of Triterpenoids and Flavonoids from Glycyrrhizae radix et rhizoma in the Human Caco-2 Monolayer Cell Model.

has been used as a traditional Chinese medicine for the treatment of various diseases. Triterpenoids and flavonoids from the plant have many beneficial effects and their chemical structures are modified in the gastrointestinal tract after oral administration. However, absorption of these triterpenoids and flavonoids still needs to be defined.

[Value of bronchoalveolar lavage as a rescue measurement for systemic lupus erythematosus complicated with diffuse alveolar hemorrhage].

Objective: To explore the value of bronchoalveolar lavage as an emergency treatment for systemic lupus erythematosus (SLE) patients with concurrent diffuse alveolar hemorrhage (DAH).

Methods: A total of 21 SLE plus DAH patients were divided randomly into 2 groups. The patients in Group A received methylprednisolone 1000 mg/d for 3 days while those in Group B methylprednisolone 1000 mg/d for 3 days in combination with a bronchoalveolar lavage.

[Investigation on the information system of immunization site at township level in Jilin province].

Objective: To understand the status of the expanded program on immunization (EPI) ralated to the immunization site at towns, and provide reliable base for it.

Methods: Questionnaires about the status of information system were filled out by all immunization site at towns, and descriptive method was applied to analyze the EPI and infomation service, personnel, equipments, funds and information system status.

Results: Among all the immunigation site in Jilin province, monthly running accounts for 75.

[Surveillance on poliomyelitis neutralization antibody level in the border areas in Jilin province].

Objective: This project is designed for understand poliomyelitis (Polio) neutralization antibody level in the the border areas in Jilin province.

Method: 263 serum samples were collected from age groups of under 1-year-old, 1-2, 3-4, 5-6, 7-14, 15-19 and above 20-years-old. Microplate neutralization test was used to determine antibody level.

[Genotype and gene charactrization of wild-type measles viruses circulated in Jilin Province].

Objective: To study the genotype and gene characterization of measles wild viruses circulated in Jilin provinces, and to provide scientific evidences for setting down controlling and preventing strategy and measures.

Methods: 38 strains of measles virus isolated in 2001-2006 were genotyped by RT-PCR-RFLP, some strains of measles virus in Jilin province were chosen for the phylogenetic analysis and for the homology analysis of nucleotide and amino acid sequences.

Results: All the 38 strains of measles virus were identified as H1 genotype by RT-PCR-RFLP, and 29 strains of them were identified further as H1 a by sequence analysis.

[Application of a simple method for the detection of measles virus genome by loop-mediated isothermal amplification (LAMP)].

Objective: A new simple RT-LAMP method was applied to detect measles virus nucleic acid and compared with nest-RT-PCR.

Methods: Compare the detection rate of the RT-LAMP method with that of nest-RT-PCR by detecting measles virus nucleic acid from measles virus and clinical samples.

Results: The nucleic acid positive rates of all 23 strains of measles virus are all 100% by the two methods.

[Study on chemical constituents in seeds of Helicia nilagirica (II)].

Objective: To study the chemical constituents in seeds of Helicia nilagirica.

Method: The ethanol extract was seperated by petroleum ether, dichloromethane, n-butanol in sequence, then isolated by silica gel column chromatography. The structures were identified and elucidated by physicochemical properties and spectral analysis.