[Effects of Chaihu Shugan San on behavior and plasma levels of corticotropin releasing hormone and adrenocorticotropic hormone of rats with chronic mild unpredicted stress depression].

Objective: To investigate the effects of Chaihu Shugan San (CHSGS), a compound traditional Chinese herbal medicine, on behavior and plasma levels of corticotropin releasing hormone (CRH) and adrenocorticotropic hormone (ACTH) of rats with chronic mild unpredicted stress depression.

Methods: Forty male Sprague-Dawley rats were randomly divided into 4 groups: normal control group, untreated group, fluoxetine group and CHSGS group. Except the normal control group, rats were singly housed and exposed to an unpredicted sequence of mild stressor for continuous 4 weeks to induce depression.

[Clinical experience of successful management of a human case with A/H5N1 infection in Hunan Province].

Objective: To study the clinical features and successful management of a survived human case with A/H5N1 infection.

Methods: The data of a confirmed case of human case with A/H5N1 infection in Hunan province were collected and analyzed.

Results: This patient with A/H5N1 infection in Hunan province was confirmed by reverse-transcription polymerase chain reaction (RT-PCR) for A/H5N1 in airway secretions.

[Correlation between serum MMP-2 and MMP-9 in patients with acute myocardial infarction before and after PCI].

Aim: To investigate the correlation between serum MMP-2 and MMP-9 in patients with acute myocardial infarction (AMI) before and after PCI.

Methods: The enzymatic activity and protein expression level of serum MMP-2 and MMP-9 in 56 patients with AMI and 20 control subjects were detected by the SDS-PAGE enzymograph and Western blot before and 1-7 days after PCI. The correlation between MMP-2 and MMP-9 was also analyzed.

[Mechanism and protective effect of glossy ganoderma decoction on the activities of RNA polymerase in hepatocyte of rabbits with Amanita mushroom poisoning].

Objective: To investigate the mechanism of glossy ganoderma decoction in Amanita mushroom poisoning.

Methods: Twenty male New Zealand white rabbits were randomly divided into 4 groups, including a normal control, a model poison group, and 2 treatment groups (different doses of glossy ganoderma decoction). The activities of hepatocyte RNA polymerase were measured by ultraviolet spectrophotometry and liver function were measured.

Clinical experience in treatment of Amanita mushroom poisoning with Glossy Ganoderma Decoction and routine Western medicines.

Objective: To assess the effects of treatment of Amanita mushroom poisoning with Glossy anoderma Decoction (, GGD).

Methods: Twelve patients with acute Amanita mushroom poisoning received conventional treatment (penicillin and reduced glutathione) combined with oral administration of GGD (treated group), which was prepared out of 200 g Glossy ganoderma decocted in water to 600 mL, and 200 ml was given once, three times a day for 7 successive days; while conventional treatment alone was given to the other 11 patients assigned to the control group. The therapeutic efficacy and changes in serum levels of total bilirubin (TBil), bile acids (BA), alanine transaminase (ALT), and aspartate transaminase (AST) activities in the two groups were compared.

[Clinical observation on treatment of Russula subnigricans poisoning patients by Ganoderma lucidum decoction].

Objective: To observe the effect of Ganoderma lucidum decoction in treating Russula subnigricans poisoning (RSP) patients.

Methods: The 14 patients of RSP in the treated group were treated with GLD (GLD, one dose was prepared by 100 g of Ganoderma lucidum decocted with water to 600 ml), on the base of conventional treatment, and 11 patients received conventional therapy in the previous year were taken as control. The clinical efficacy and parameters in them were compared, including the urine N-acetyl-D-glucosaminidase (NAG, which reflects the injury of kidney), the red blood cell and protein in urine, the alanine transaminase (ALT, which reflects the injury of liver), and the aspartate aminotransferase (AST, which reflects the injury of heart).