Ghrelin, neuropeptide Y (NPY) and cholecystokinin (CCK) in blunt snout bream (Megalobrama amblycephala): cDNA cloning, tissue distribution and mRNA expression changes responding to fasting and refeeding.

Blunt snout bream (Megalobrama amblycephala Yih, 1955) is an endemic freshwater fish in China for which the endocrine mechanism of regulation of feeding has never been examined. Ghrelin, neuropeptide Y (NPY) and cholecystokinin (CCK) play important roles in the regulation of fish feeding. In this study, full-length cDNAs of ghrelin, NPY and CCK were cloned and analyzed from blunt snout bream.

Genetic diversity of and differentiation among five populations of blunt snout bream (Megalobrama amblycephala) revealed by SRAP markers: implications for conservation and management.

The blunt snout bream (Megalobrama amblycephala) is an important freshwater aquaculture fish throughout China. Because of widespread introductions of this species to many regions, the genetic diversity of wild and natural populations is now threatened. In the present study, SRAP (sequence-related amplified polymorphism) markers were used to assess genetic diversity of blunt snout bream.

Complete mitochondrial DNA genome of triploid pengze crucian carp (Carassius auratus).

The complete mitochondrial genome sequence of triploid pengze crucian carp was 16,580 bp in length. The mitochondrial genome contains 22 transfer RNAs, 13 protein-coding genes, 2 ribosomal RNAs and 2 non-coding regions (control region and origin of light-strand replication). All genes were encoded on the heavy strain except for ND6 and eight tRNA genes.

Complete mitochondrial DNA genome of tetraploid Carassius auratus gibelio.

The complete mitochondrial genome was sequenced from the tetraploid Carassius auratus gibelio in this study. The genome sequence was 16,576 bp in length. The mitochondrial genome contains 13 protein-coding genes, 2 ribosomal RNAs, 22 transfer RNAs, and 2 non-coding regions (control region and origin of light-strand replication).

Complete mitochondrial DNA genome of Pseudobagrus medianalis (Siluriformes: Bagridae).

The complete mitochondrial genome was sequenced from the freshwater fish, Pseudobagrus medianalis (Siluriformes: Bagridae) in this study. The genome sequence was 16,647 bp in length, and the gene order and contents were identical with the bagridae fishes. The mitochondrial genome contains 13 protein-coding genes, 2 ribosomal RNAs, 22 transfer RNAs and 2 non-coding regions (control region and origin of light-strand replication).

Complete mitochondrial genome of Yangtze River wild common carp (Cyprinus carpio haematopterus) and Russian scattered scale mirror carp (Cyprinus carpio carpio).

We sequenced the complete mitogenomes of (Cyprinus carpio haematopterus) and Russian scattered scale mirror carp (Cyprinus carpio carpio). Comparison of these two mitogenomes revealed that the mitogenomes of these two common carp strains were remarkably similar in genome length, gene order and content, and AT content. There were only 55 bp variations in 16,581 nucleotides.

Complete mitochondrial genome of Xingguo red carp (Cyprinus carpio var. singuonensis) and purse red carp (Cyprinus carpio var. wuyuanensis).

The complete mitochondrial genomes of Xingguo red carp (Cyprinus carpio var. singuonensis) and purse red carp (Cyprinus carpio var. wuyuanensis) were sequenced.

Molecular cloning and expression analysis of two β-defensin genes in the blunt snout bream (Megalobrama amblycephala).

β-Defensins are a group of cysteine-rich, cationic antimicrobial peptides that play important roles in innate immune system against pathogenic microbes invading. In this study, the part-length cDNA sequences of two β-defensin genes (maΒD-1, maΒD-2) in blunt snout bream (Megalobrama amblycephala) were identified. Homology analysis showed that the cDNA sequences of maΒD-1 and maΒD-2 had high similarities to those in common carp and zebrafish.

Molecular cloning and expression analysis of liver-expressed antimicrobial peptide 1 (LEAP-1) and LEAP-2 genes in the blunt snout bream (Megalobrama amblycephala).

Liver-expressed antimicrobial peptide 1 (LEAP-1) and LEAP-2 are widespread in fish and extremely important components of the host innate immune system. In this study, full-length cDNAs of LEAP-1 and LEAP-2 were cloned and sequenced from blunt snout bream, Megalobrama amblycephala. The open reading frames (ORF) of LEAP-1 and LEAP-2 genes encode putative peptides of 94 and 92 amino acids, which possess eight and four conserved cysteine residues, respectively.

Complete mitochondrial DNA genome of Pseudobagrus brevicaudatus (Siluriformes: Bagridae).

The complete mitochondrial genome of Pseudobagrus brevicaudatus (Siluriformes: Bagridae) was sequenced in this study. The total length of the mitogenome is 16,533 bp, with the base composition of 31.6% A, 26.

Complete mitochondrial DNA genome of Pseudobagrus truncatus (Siluriformes: Bagridae).

In this study, the complete mitochondrial DNA (mtDNA) sequence of Pseudobagrus truncatus (Siluriformes: Bagridae) was determined. The complete mtDNA genome sequence of P. truncatus is 16,533 bp in size.

Mitochondrial genome sequence of the Botia superciliaris (Teleostei, Cypriniformes).

The Botia superciliaris is endemic to China and distributed mainly in the upper reaches of the Yangtze River. The complete mitochondrial genome was 16,597 bp in length, which contained 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and 1 displacement loop (D-loop) locus. Except for the ND6 gene and eight tRNA genes, all other mitochondrial genes were encoded on the heavy strand.

Complete mitochondrial DNA genome of Leiocassis crassilabris(Siluriformes: Bagridae).

The polymerase chain reaction method was employed to obtain the complete mitochondrial genome of Leiocassis crassilabris from the Yangtze River, China. The mitogenome of L. crassilabris is 16,534 bp in length, consisting of 37 genes coding for 13 proteins, 22 transfer RNAs, 2 ribosomal RNAs, and 1 control region.

Mitochondrial genome of the Shorthead catfish (Pelteobagrus eupogon).

The complete mitochondrial genome of Shorthead catfish (Pelteobagrus eupogon) was obtained by DNA sequencing based on the PCR fragments. The entire genome was 16,562 bp in length, with a gene content of 13 protein-coding, 2 ribosomal RNA, and 22 transfer RNA genes, and order identical to that observed in most other vertebrates. Those genes are arranged compactly with no introns and few intergenic nucleotides.

Mitochondrial genome sequence of the shining catfish (Pelteobagrus nitidus).

The complete mitochondrial DNA sequence of Pelteobagrus nitidus was determined using a PCR-based method. The total length of mitochondrial DNA is 16,532 bp. The contents of the P.

Mitochondrial DNA sequence of yellow catfish (Pelteobagrus fulvidraco).

Yellow catfish (Pelteobagrus fulvidraco) belongs to the family Bagridae, which is one of the most important economic freshwater aquaculture species in China. In this study, we reported the complete sequence of the mitochondrial genome of P. fulvidraco.

[Polymorphism of MSTN gene and its association with growth traits in yellow catfish (Pelteobagruse fulvidraco)].

Myostatin (MSTN) gene has negative influence on the growth of muscle, which belongs to transforming growth factor-β family. Using single-strand conformation polymorphism (SSCP), single nucleotide polymorphisms (SNPs) of MSTN gene were detected in yellow catfish. Correlation analysis between MSTN polymorphisms and growth traits was also carried out.

[Sequence and phylogeny analysis of the complete mitochondrial genome of Pelteobagrus vachelli].

Morphological classification of Bagridae fishes is relatively difficult due to various kinds and similar shape, and the phyletic evolution is not very clear in some species. To provide basic data to the classification of Bagridae and Siluriformes fishes, the complete mitochondrial genome of Pelteobagrus vachelli was obtained by PCR based on 16 primers, which were designed on the basis of related species mtDNA sequences. The complete mitochondrial genome is 16 527 bp in length, including 2 ribosomal RNA genes, 22 transfer RNA genes, 13 protein-coding genes, and a non-coding control region.

Molecular cloning and differential expression patterns of copper/zinc superoxide dismutase and manganese superoxide dismutase in Hypophthalmichthys molitrix.

Copper/zinc superoxide dismutase (Cu,Zn-SOD) cDNA and manganese superoxide dismutase (Mn-SOD) cDNA were first cloned from silver carp Hypophthalmichthys molitrix using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) method. The open reading frame (ORF) of Cu,Zn-SOD is 465 bp and encodes a 154 amino acids (aa) protein, whereas the ORF of Mn-SOD is 675 bp and encodes a 224 aa protein. Multiple polypeptide sequence alignment showed high identity both of Cu,Zn-SOD (70-87%) and Mn-SOD (80-96%) with the species compared.

[Improvement of the silver-stained technique of polyacrylamide gel electrophoresis.].

Polyacrylamide gel electrophoresis is widely applied in the SSR, SNP markers analysis and the construction of genetic map. It is disadvantage to use conventional silver-stained methods in experiment due to taking a very long time and having fussy steps. In this paper, a silver-stained method of DNA in PAGE gel was improved.

[Genetic variation analysis of two silver carp populations in the middle and upper Yangtze River by microsatellite.].

Thirty nine microsatellite markers were applied to analyze the genetic diversity of two silver carp populations in the middle and upper reaches of the Yangtze River. The following parameters were calculated: average observed number of alleles, average effective number of alleles, genetic heterozygosity, polymorphism information content (PIC), genetic similarity coefficient, genetic distance, Hardy-Weinberg balance deflection index. These results indicated that the average observed number of alleles in each microsatellite locus of the Wanzhou population and Jianli populations is 6.