[Gene expression and activities analysis of a new fusion protein (RGD) 3/tTF].

To develop a new fusion protein (RGD)3/tTF for the therapy of the selective thrombosis of tumor blood vessels. The fused gene (RGD) 3/tTF was reconstructed by PCR, was cloned into vector pET22 b(+), and expressed in E. coli BL21 (DE3).

[Gene's construction, expression and activity analysis of humanized single-domain antibody against human lung cancer].

Aim: To construct the gene of humanized single-domain antibody hu3D3V(H) against human lung cancer, to express it in E.coli and analyze its activity.

Methods: The mAb3D3V(H) was humanized by using CDRs grafting technique.