[Analysis of the main components of inner ear antigens inducing autoimmune Meniere's disease in guinea pigs].

Objective: To investigate the main components of inner ear antigens inducing autoimmune Meniere's disease (AIMD) in guinea pigs.

Methods: The guinea pigs were immunized with isologous crude inner ear antigens (ICIEAg). Then, the hearing function was measured with auditory brainstem response (ABR), the vestibular function was measured with electronystagmography (including spontaneous nystagmus and caloric test), and inner ear histopathological changes were observed by inner ear celloidin section with haematoxylin-eosin staining and observed under light microscope.

[Expression of KISS-1 and GnRH in rat hypothalamus].

Objective: To assay the expression of KiSS-1 and GnRH in the male rat hypothalamus at different developmental stages, and to explore the significance of KiSS-1 in sex development onset and normal reproduction regulation.

Methods: Expression analyses of KiSS-1 and GnRH genes were conducted in the rat hypothalamus at different developmental stages with RT-PCR and real time-PCR. The testosterone level was assayed by chemoluminescence technique.