An Immuno-Separated Assay for Ochratoxin Detection Coupled with a Nano-Affinity Cleaning-Up for LC-Confirmation.

An immuno-separated assay for ochratoxin A detection coupled with a nano-affinity cleaning up for LC-confirmation was developed. Firstly, ochratoxin A was modified to quantum dot beads for immuno-fluorescent reporters. Secondly, FeO magnetic nanoparticles were conjugated with protein G for immuno-magnetic adsorbents.

1H-Pyrrole-2-carboxylic acid.

In the title compound, C(5)H(5)NO(2), the pyrrole ring and its carboxyl substituent are close to coplanar, with a dihedral angle of 11.7 (3)° between the planes. In the crystal structure, adjacent mol-ecules are linked by pairs of O-H⋯O hydrogen bonds to form inversion dimers.

1-Ethyl-1H,6H-pyrrolo[2,3-c]azepine-4,8(5H,7H)-dione.

The title compound, C(10)H(12)N(2)O(2), was synthesized by cyclization of 3-(1-ethyl-pyrrole-2-carboxamido)propanoic acid in the presence of polyphospho-ric acid and diphospho-rus pentoxide. In the crystal structure, adjacent mol-ecules are linked by N-H⋯O hydrogen bonds, forming chains extending along the b axis.

3-(1-Ethyl-1H-pyrrole-2-carboxamido)propionic acid monohydrate.

The title compound, C(10)H(14)N(2)O(3)·H(2)O, was synthesized by alkyl-ation of methyl 3-(1H-pyrrole-2-carboxamido)-propion-ate with ethyl bromide, followed by saponification and acidification. In the crystal structure, inter-molecular O-H⋯O and N-H⋯O hydrogen bonds link the mol-ecules, forming layers parallel to the ac plane.

Methyl 3-[(1-butyl-1H-indol-3-yl)carbonyl-amino]propionate.

In the title mol-ecule, C(17)H(22)N(2)O(3), the mean plane of the terminal (C=O)OMe fragment and the indole plane form a dihedral angle of 78.94 (3)°. Inter-molecular N-H⋯O hydrogen bonds link the mol-ecules into chains extended along the c axis.

Methyl (1H-pyrrol-2-ylcarbonyl-amino)acetate.

In the crystal structure of the title compound, C(8)H(10)N(2)O(3), mol-ecules are linked by N-H⋯O hydrogen bonds, forming ribbons of centrosymmetric dimers extending along the c axis.