[Establishment of rat integrated discrete multiple organ cell culture (IdMOC) model].

Objective: To establish the integrated discrete multiple organ cell culture (IdMOC) system.

Methods: Rat primary cell of hepatocyte, nephrocyte, cardiomyocytes, alveolar macrophage, dermal fibroblasts were isolated by collagenase digestion, separation of bronchial lavage, two-step digestion method and cultured respectively, with monolayer culture. To establish the integrated discrete multiple organ cell culture (IdMOC) system, glass slides of five different cells were used to the same dish with 10% FBS DMEM medium cultured 7d, using MTT comparison primary cells cultured alone and cocultured when growth.

Major achievements in relation to dyslexia in Chinese characters.

Objective: To review the major achievements in dyslexia in Chinese characters, hoping to give some clues for future studies.

Data Sources: Both Chinese and English language literature search using PUBMED, and original articles published in main Chinese and international journals.

Study Selection: After reviewing the literature, 54 articles were selected that specifically addressed the stated purpose.

[Identification and tissue localization of intermediate filament protein in Angiostrongylus cantonensis].

Objective: To identify the type of the intermediate filament (IF) protein of Angiostrongylus cantonensis and analyze its tissue localization.

Methods: Recombinant pET-IF of antigen IF was expressed in E.coli with IPTG induction, and the expression products were purified by His.