Identification of chemical inhibitors targeting long noncoding RNA through gene signature-based high throughput screening.

Scalable methods for functionally high-throughput screening of RNA-targeting small molecules are currently limited. Here, an RNA knockdown gene signature and high-throughput sequencing-based high-throughput screening (HTS) were integrated to identify RNA-targeting compounds. We first generated a gene signature characterizing the knockdown of the long non-coding RNA LINC00973.

miR-135a-5p alleviates cerebral ischemia-reperfusion injury by inhibiting pyroptosis mediated through the DDX3X/NLRP3 pathway.

MicroRNAs (miRNAs) are widely involved in signal transduction and regulation during cerebral ischemia-reperfusion injury (CIRI). This study investigates the molecular mechanisms of the specific miRNA/DDX3X/NLRP3 pathway in early-stage CIRI and explores its potential clinical applications. Through public database analysis, miR-135a-5p targeting DDX3X after CIRI was determined.

Chemerin attenuates acute kidney injury by inhibiting ferroptosis via the AMPK/NRF2/SLC7A11 axis.

Acute kidney injury (AKI) is a common and life-threatening condition associated with cell death, where ferroptosis plays a critical role. Chemerin, primarily produced in white adipose tissue, has multiple biological functions in renal pathophysiology. However, to date, whether and how chemerin regulates the progression of AKI remain unclear.

Effects of soy flour formulation and pretreatment on the properties of gluten-free cookies: A comprehensive study from flour, dough, to baked products.

Grain-based gluten-free cookies are often nutritionally inferior owing to their low protein content. This study aimed to enhance the nutritional value of gluten-free cookies by incorporating soy flour and to investigate the effects of different types of modified soy flour on the properties of gluten-free dough and cookies. Results indicate that all types of modified soy flour significantly decreased water absorption capacity (p < 0.

A novel dicyanoisophorone-based fluorescent probe for rapid detection of acetylcholinesterase in biological systems.

Acetylcholinesterase (AChE) plays a vital role in various neurological diseases including brain disorders, neurotransmission alterations, and cancer. Developing effective methods to image AChE in biological samples is essential for understanding its mechanisms in biosystems. Here, we introduce a novel fluorescent probe CNA, that enables detection of AChE at 520 nm with rapid response time of 60 s and a detection limit of 0.

Acteoside relieves diabetic retinopathy through the inhibition of Müller cell reactive hyperplasia by regulating TXNIP and mediating Kir4.1 channels in a PI3K/Akt-dependent manner.

Diabetic retinopathy (DR) is a severe microangiopathy of diabetes. Müller cells play an important role in the development of DR. Acteoside (ACT) has been reported to be effective in the treatment of DR.

The decline in tropical land carbon sink drove high atmospheric CO growth rate in 2023.

Atmospheric CO growth rate (CGR), reflecting the carbon balance between anthropogenic emissions and net uptake from land and ocean, largely determines the magnitude and speed of global warming. The CGR at Mauna Loa Baseline Observatory reached a record high in 2023. We quantified major components of the global carbon balance for 2023, by developing a framework that integrated fossil fuel CO emissions data and an atmospheric inversion from the Global ObservatioN-based system for monitoring Greenhouse GAses (GONGGA) with two artificial intelligence (AI) models derived from dynamic global vegetation models.

A Nattokinase-Loaded Nanozyme for Alleviating Acute Myocardial Infarction via Thrombolysis and Antioxidation.

Acute myocardial infarction (MI) induced by thrombus formation is a prevalent cardiovascular disorder, and thrombolytic therapy continues to be a principal treatment modality. Prior research indicates a substantial association among MI, thrombosis, and the activation of oxidative stress pathways. The effectiveness of current thrombolytic drugs is relatively constrained, and the need for innovative and versatile thrombolytic medications remains critical.

fMRI used to observe the acute craniocerebral response of esophageal cancer related depressive patients treated by rTMS: Initial experience.

To observe the immediate craniocerebral response, changes of spontaneous nerve activity and functional connection after repeated transcranial magnetic stimulation (rTMS) in esophageal cancer patients with depression (ECPD) by functional magnetic resonance imaging (fMRI), and to explore the therapeutic effect, neuroactivity response and mechanism. Eleven patients with ECPD were enrolled to treated with single rTMS. The patients were examined by fMRI before and after the treatment.

Single-tube detection of a foodborne bacterial pathogen using user-friendly portable device.

Timely and reliable detection of foodborne bacterial pathogen is crucial for reducing disease burden in low- and middle-income countries. However, laboratory-based methods are often inaccessibility in resource-limited settings. Here, we developed a single-tube assay and a low-cost palm-sized device for on-site detection of the representative foodborne bacterial pathogen, Salmonella Enteritidis.

A xanthene-based fluorescent probe capable of selectively detecting phosgene in living mouse model.

In this study, we introduced a novel fluorescent probe RNP, which demonstrated outstanding sensitivity and specificity for phosgene detection. Upon phosgene exposure, the probe exhibited rapid fluorescence turn-on within a short re-sponse time (6 s). RNP was also effective in both cellular and murine experiments, where fluorescence enhancement was observed in the respiratory tract of mice.

Palmitoyl copper peptide and acetyl tyrosine complex enhances melanin production in both A375 and B16 cell lines.

Copper peptide, a low molecular weight peptide composed of glycyl-L-histidyl-l-lysine-copper, possesses anti-aging, anti-inflammatory, and wound healing properties. In this study, we investigated the effects of a combination agent CP-AcT, composed of palmitoyl copper peptide (pal-GHK-Cu) and acetyl tyrosine (N-Acetyl-l-tyrosine), on melanin production in the human malignant melanoma cell line A375 and the mouse melanoma cell line B16. Firstly, the cytotoxicity of CP-AcT at various concentrations (0-8 μg/mL) on HaCat, HFF, A375, and B16 cells was evaluated.

In situ engineered CeOS/CeO nanofibrous heterojunctions for photocatalytic HO synthesis via S-scheme charge separation.

Photocatalytic HO synthesis offers an efficient and sustainable means to convert solar energy into chemical energy, representing a forefront and focal point in photocatalysis. S-scheme heterojunctions demonstrate the capability to effectively separate photogenerated electrons and holes while possessing strong oxidation and reduction abilities, rendering them potential catalysts for photocatalytic HO synthesis. However, designing S-scheme heterojunction photocatalysts with band alignment and close contact remains challenging.

[Integrated Three-steps Method for Identifying Priority Pollutants in Reclaimed Water].

Trace organic contaminants （TrOCs） in reclaimed water can pose potential ecological and health risks during long-term reuse, but the current water quality standards have not restricted their limits. For preventing and controlling the potential risks from TrOCs, an integrated method EHL for identifying priority pollutants in reclaimed water was proposed. This method followed three steps： First, a meta-analysis was performed to assess the exposure of TrOCs in reclaimed water, and a database of TrOCs exposure （E） was established.

Psychosis and the self: How spontaneous discussions of subjective experiences compare in the clinical high-risk and first-episode psychosis populations.

Objective: Prior qualitative studies show that individuals with psychoticlike experiences express difficulties concerning their identity. However, previous work has studied individuals at clinical high risk for psychosis (CHR) and individuals with first-episode psychosis (FEP) separately. Here, we compare the experiences of individuals at CHR, individuals with FEP, and healthy individuals.

Differential Expressions of circRNAs and Regulatory Mechanisms of ceRNA Network in Liver of Wilson's Disease TX Mice.

Background: Wilson's disease (WD) is a hereditary disorder characterized by an abnormality in copper metabolism. Liver fibrosis, and potentially cirrhosis, induced by copper accumulation are critical factors in the pathogenesis of WD. CircRNAs exhibit high stability and play crucial roles in numerous biological processes.

METTL16 is Required for Meiotic Sex Chromosome Inactivation and DSB Formation and Recombination during Male Meiosis.

Meiosis in males is a critical process that ensures complete spermatogenesis and genetic diversity. However, the key regulators involved in this process and the underlying molecular mechanisms remain unclear. Here, we report an essential role of the mA methyltransferase METTL16 in meiotic sex chromosome inactivation (MSCI), double-strand break (DSB) formation, homologous recombination and SYCP1 deposition during male meiosis.

Ventral Hippocampal CA1 GADD45B Regulates Susceptibility to Social Stress by Influencing NMDA Receptor-Mediated Synaptic Plasticity.

Growth arrest DNA damage-inducible protein 45 β (GADD45B) has been reported to be a regulatory factor for active DNA demethylation and is implicated in the modulation of synaptic plasticity and chronic stress-related psychopathological processes. However, its precise role and mechanism of action in stress susceptibility remain elusive. In this study, we found a significant reduction in GADD45B expression specifically in the ventral, but not the dorsal hippocampal CA1 (dCA1) of stress-susceptible mice.

Association Between Preoperative Lymphocyte-to-Monocyte Ratio and Occurrence of Postoperative Cognitive Dysfunction: A Prospective Cohort Study.

Purpose: Postoperative cognitive dysfunction (POCD) is a common postoperative complication. Studies have reported that lymphocyte-to-monocyte ratio (LMR) was a predictor of many diseases associated with inflammation. However, further examination of the relationship between preoperative LMR and POCD is needed.

Metabolic Chaos in Kidney Disease: Unraveling Energy Dysregulation.

Background: Acute kidney injury (AKI) and chronic kidney disease (CKD) share a fundamental disruption: metabolic dysfunction.

Methods: A literature review was performed to determine the metabolic changes that occur in AKI and CKD as well as potential therapeutic targets related to these changes.

Results: In AKI, increased energy demand in proximal tubular epithelial cells drives a shift from fatty acid oxidation (FAO) to glycolysis.

Predicting OSA Using Radiographs of the Airway Anatomy.

Objective: This study aims to analyze the tongue body shape and upper airway anatomical parameters in patients with Obstructive Sleep Apnea (OSA) and to explore the anatomical causes of OSA.

Methods: A total of 345 subjects participated in this study. Lateral pharyngeal images of the upper respiratory tract were captured in both normal and mandibular advancement states using X-ray plain film.

Fluorescent probes for sensing peroxynitrite: biological applications.

Peroxynitrite (ONOO) is a quintessential reactive oxygen species (ROS) and reactive nitrogen species (RNS), renowned for its potent oxidizing and nitrifying capabilities. Under normal physiological conditions, a baseline level of ONOO is present within the body. However, its production escalates significantly in response to oxidative stress.

Experiences and perceptions of fear of falling in older adults: A systematic review and meta-synthesis of qualitative studies.

Objective: To examine and synthesize qualitative data about the experiences of fear of falling in older adults.

Method: This review used the Joanna Briggs methodology for qualitative systematic reviews. The PubMed, Cochrane Library, Embase, Web of Science, PsycINFO, CINAHL, CBMdisc, CNKI, Wanfang, and VIP databases were searched up to December 2023.

Single-cell sequencing reveals transcriptional dynamics regulated by ERα in mouse ovaries.

Context: Estrogen receptor α (ERα) is a key regulator of reproductive function, particularly in ovarian development and function, yet the specifics of its role at the molecular level remain unclear.

Aims: The study aims to elucidate the molecular mechanisms of ERα-regulated transcriptional dynamics in ovarian cells using ERα knockout (αERKO) mice created via CRISPR/Cas9.

Methods: Single-cell RNA sequencing (scRNA-seq) was used to compare transcriptomes from individual ovarian cells in both wild type and αERKO mice.