Mutagenesis of Odorant Receptor Coreceptor Reveals the Odorant-Detected Behavior of the Predator .

The successful mating of the hoverfly and the search for prey aphids are of great significance for biological control and are usually mediated by chemical cues. The odorant receptor co-receptor () genes play a crucial role in the process of insect odor perception. However, the function of in the mating and prey-seeking behaviors of the hoverfly remains relatively unexplored.

Ultrastructural and Descriptive Study on the Adult Body Surface of (Lepidoptera: Crambidae).

Moore, 1885 (Lepidoptera: Crambidae) is an economically important lepidopteran pest that caused severe damage to the plantation area of (Lour.) Gilg, 1825 (Thymelaeaceae), resulting in extensive defoliation of the trees during an epidemic. In this study, we used scanning electron microscopy (SEM) to analyze the external morphology and ultrastructure of sensilla on various body parts of .

Development of a New Sex Attractant via the Peripheral Coding of Pheromones in .

Sex pheromones play an essential role when moths are searching for mates. Male olfactory receptor neurons (ORNs) are the primary determinant during peripheral pheromone recognition. Here, we identified the sex pheromones of a global agricultural pest, , using gas chromatography coupled with mass spectrometry and electroantennographic detection.

Conserved Odorant Receptor, EcorOR4, Mediates Attraction of Mated Female to 1-Octen-3-ol.

Odorant receptors (ORs) in insects are crucial for the detection of chemical signals. However, the functions of the conserved OR genes among insect species are rarely studied. In this study, we analyzed a well-conserved OR clade in Diptera insects and cloned a gene from this clade, , in the hoverfly .

Functional characterization of sex pheromone receptors in Spodoptera frugiperda, S. exigua, and S. litura moths.

Moths possess an extremely sensitive and diverse sex pheromone processing system, in which pheromone receptors (PRs) are essential to ensure communication between mating partners. Functional properties of some PRs are conserved among species, which is important for reproduction. However, functional differentiation has occurred in some homologous PR genes, which may drive species divergence.

Identification and Functional Characterization of Sex Pheromone Receptors in the Oriental Fruit Moth, (Lepidoptera: Tortricidae).

The oriental fruit moth, , is a worldwide pest that damages Rosaceae fruit trees. Sex pheromones play an important role in controlling this pest; however, the corresponding chemosensation mechanism is currently unknown. In this study, 60 candidate odorant receptors, including eight pheromone receptors (PRs), were identified by antennal transcriptome analysis.

Functional map of the macroglomerular complex of male Helicoverpa armigera.

The mechanism of sex pheromone reception in the male cotton bollworm Helicoverpa armigera has been extensively studied because it has become an important model system for understanding insect olfaction. However, the pathways of pheromone processing from the antenna to the primary olfactory center in H. armigera have not yet been clarified.

[Efficacy and safety of black tomato concentrate in the treatment of erectile dysfunction: A prospective randomized controlled open trial].

Objective: To evaluate the efficacy and safety of black tomato concentrate (BTC), which is rich in polyphenols, in the treatment of ED.

Methods: We conducted a prospective randomized open clinical study of 150 ED patients from December 2018 to February 2020, and treated the them with placebo (n = 50), BTC (n = 50) and Compound Xuanju Capsules (CXC) (n = 50), respectively, all for 8 weeks. Before and at 4 and 8 weeks after treatment, we obtained the scores of the patients on IIEF-5, Erection Hardness Score (EHS), Sexual Encounter Profile (SEP-2,3) and General Assessment Questionnaire (GAQ-1,2), related biochemical indexes and the T level, followed by comparison among the three groups.

Optimization of a pheromone lure by analyzing the peripheral coding of sex pheromones of Spodoptera frugiperda in China.

BACKGOUND Sex pheromones of the fall armyworm, Spodoptera frugiperda, show differences in composition and proportions in different geographical populations, but always contain Z9-14:OAc as the major component. Odorant receptor neurons (ORNs) housed in the long trichoid sensilla (TS) of male antennae are essential to detect female-released sex pheromones in moths. RESULTS: In this study, we identified seven components from pheromone gland extracts of female S.

Ionotropic receptor 8a is involved in the attraction of Helicoverpa armigera to acetic acid.

Ionotropic receptors (IRs) were first found in Drosophila melanogaster, and derive from ionotropic glutamate receptors (iGluRs), which are implicated in detecting acids, ammonia, amine, temperature and humidity. Although IRs are involved in sensing acid odors in a few insects, such as D. melanogaster, Aedes aegypti, and Manduca sexta, the function of IRs in Helicoverpa armigera is still unknown.

Neuronal architecture and functional mapping of the taste center of larval Helicoverpa armigera (Lepidoptera: Noctuidae).

The sense of taste plays a crucial role in herbivorous insects by discriminating nutrients from complex plant metabolic compounds. The peripheral coding of taste has been thoroughly studied in many insect species, but the central gustatory pathways are poorly described. In the present study, we characterized single neurons in the gnathal ganglion of Helicoverpa armigera larvae using the intracellular recording/staining technique.

Identification and physiological function of CsPrip, a new aquaporin in Chilo suppressalis.

Aquaporin (AQP) transport solutes across cell membranes in both unicellular and multicellular organisms. In this study, the aquaporin CsPrip was identified in Chilo suppressalis, an important pest of rice. CsPrip was comprised of two variants, CsPrip_v1 and CsPrip_v2; the former variant was <103 bp was shorter than the latter, although both exhibited the same open reading frame (ORF).

Distinct protocerebral neuropils associated with attractive and aversive female-produced odorants in the male moth brain.

The pheromone system of heliothine moths is an optimal model for studying principles underlying higher-order olfactory processing. In , three male-specific glomeruli receive input about three female-produced signals, the primary pheromone component, serving as an attractant, and two minor constituents, serving a dual function, that is, attraction versus inhibition of attraction. From the antennal-lobe glomeruli, the information is conveyed to higher olfactory centers, including the lateral protocerebrum, via three main paths - of which the medial tract is the most prominent.

Aromatic Volatiles and Odorant Receptor 25 Mediate Attraction of to Flowers.

Flowering plants attract pollinators with volatile chemicals that include aromatic compounds. Syrphid flies are the largest group of flower visitors in Diptera, but little is known about how they detect floral scents at the molecular level. Here, electroantennogram (EAG) recordings from the antennae of were used to measure responses from 14 aromatic compounds.

Functional characterization of pheromone receptor candidates in codling moth Cydia pomonella (Lepidoptera: Tortricidae).

Sex pheromones serve a critical role in Lepidopterans finding mates. Male moths perceive and react to sex pheromones emitted by conspecific females through a delicate pheromone communication system. Pheromone receptors (PRs) are the key sensory elements at the beginning of that process.

Revisiting the Labial Pit Organ Pathway in the Noctuid Moth, .

Lepidopteran species detect CO via a specialized organ located on the peripheral segment of the labial palps, the labial palp pit organ (LPO). Based on tracing of LPO sensory neurons targeting one distinct antennal-lobe glomerulus, Kent and her colleagues described the projections originating from the LPO in the sphinx moth as "" already in the 1980 ties. In spite of similar reports from studies of other lepidopteran species, however, it has been an unresolved issue whether additional termination areas of the labial nerve, such as the gnathal ganglion (GNG) and the ventral nerve cord, are actually output sites of LPO neurons.

Evaluating the ecotoxicological effects of Pb contamination on the resistance against Lymantria dispar in forest plant, Larix olgensis.

Background: Heavy metal contamination in forest ecosystems has become increasingly severe, and there is an urgent need to better understand the ecotoxicological effects of heavy metals on the whole forest ecosystems, especially their effects on insect resistance of forest plant. In the present study, the resistance against gypsy moth (Lymantria dispar) larvae in Larix olgensis seedlings grown in non-amended or Pb-amended (at 500 and 1500 mg kg ) soil was evaluated.

Results: Pb from the treated soil could be transferred and exerted bio-toxicological effects along the food chain consisting of L.

The improved resistance against gypsy moth in Larix olgensis seedlings exposed to Cd stress association with elemental and chemical defenses.

Background: Cadmium (Cd), as an environmental pollutant, can endanger various biological and chemical characteristics of plants in multiple aspects. In this study, the effects of Cd contamination or exposure for 30 and 60 days at 1, 2, 4 mg kg concentrations on the resistance of Larix olgensis seedlings to the gypsy moth (Lymantria dispar) larvae were investigated.

Results: Our results showed that Cd stress did not significantly affect the growth and biomass parameters of the larch seedlings, which might be attributed to the scavenging mechanism of reactive oxygen species (e.

Development and Clinical Validation of Multiple Cross Displacement Amplification Combined With Nanoparticles-Based Biosensor for Detection of : Preliminary Results.

Tuberculosis is still a major threat to global public health. Here, a novel diagnosis assay, termed as multiple cross displacement amplification combined with nanoparticle-based lateral flow biosensor (MCDA-LFB), was developed to simultaneously detect IS and IS of (MTB) in DNA extracted from reference strain H37Rv and clinical samples. The amplification can be finished within 30 min at a fixed temperature (67°C), thus the whole procedure, including rapid template preparation (15 min), isothermal reaction (30 min) and result reporting (2 min), can be completed within 50 min.