Publications by authors named "Gui Lian Li"
Article Synopsis
- * Researchers analyzed 146 isolates, identifying various mutations and assessing their impact on EMB resistance through minimum inhibitory concentration testing and statistical modeling.
- * Results showed that certain mutations (Met306Val, Met306Ile, Gly406Ala, and Gln497Arg) were significantly related to EMB resistance, with some mutations strongly correlating with high-level resistance, highlighting the complex genetic factors influencing EMB susceptibility.
Article Synopsis
- A new assay called MIRA-LF was developed to quickly and accurately detect fluoroquinolone (FQ) resistance in tuberculosis patients.
- The assay identifies mutations in specific codons of the gyrA gene, which are associated with resistance to levofloxacin (LFX).
- MIRA-LF showed significantly high sensitivity (92.4%), specificity (98.5%), and accuracy (96.5%) compared to traditional testing methods, making it particularly beneficial for use in resource-limited settings.
[Main Infection Control Measures for Respiratory Infectious Diseases in Medical Institutions and Public Places in China].
Zhongguo Yi Xue Ke Xue Yuan Xue Bao
April 2023
Article Synopsis
- Respiratory infectious diseases (RID) pose significant public health threats, and effective infection control (IC) is crucial for managing their spread.
- A review of literature since 2018 highlighted achievements, problems, and challenges in IC across various levels of medical institutions in China, showing inconsistent efforts in different regions.
- Recommendations include better application of existing IC tools, the development of advanced technology for new IC products, and the establishment of a digital platform for monitoring and managing infections to enhance IC measures.
Article Synopsis
- The study aimed to investigate how specific genetic mutations relate to resistance against the antibiotics rifampin (RIF) and rifabutin (RFB) in bacteria.
- Researchers analyzed 177 clinical isolates, identifying multiple mutations and their impact on the minimum inhibitory concentrations (MICs) for RIF and RFB.
- The findings revealed distinct mutation patterns linked to high-level resistance, underscoring the complexity of rifamycin resistance and its implications for tuberculosis treatment options.*
Mutations and Effects on Rifampin Resistance in .
Ma-Chao Li Jie Lu Yao Lu Tong-Yang Xiao Hai-Can Liu Shi-Qiang Lin Gui-Lian Li Zhi-Guang Liu Li-Li Zhao Kang-Lin Wan
Infect Drug Resist
October 2021
Article Synopsis
- - The study aimed to explore mutations in the RpoB gene and their role in developing resistance to rifampin (RIF), using structural analysis.
- - Researchers sequenced the RpoB gene in 175 tuberculosis isolates and identified 34 mutations across 17 sites; many of these mutations impact the interaction between RpoB and RIF.
- - Key mutations like S450L and H445D were linked to high-level RIF resistance, offering insights that could inform the creation of new antibiotics and diagnostic methods for tuberculosis.
Article Synopsis
- The study aimed to assess the immunogenicity of proteins and identify cross-reactive proteins related to two different pathogens.
- Mice were immunized with protein extracts, showing significant immune responses, including increased cytokine and immunoglobulin levels, and better control of bacterial loads.
- Genome analysis identified 396 common genes and 60 cross-reactive antigens between the pathogens, suggesting potential targets for developing new tuberculosis vaccines.
[A comparative study of cerebellar development between appropriate-for-gestational age infants and small-for-gestational-age infants].
Zhongguo Dang Dai Er Ke Za Zhi
September 2020
Article Synopsis
- The study aims to compare cerebellar development between appropriate-for-gestational-age (AGA) and small-for-gestational-age (SGA) infants.
- Researchers examined 165 AGA and 105 SGA infants using ultrasound to measure various aspects of cerebellar size and development shortly after birth.
- Results showed that while early SGA infants (under 34 weeks) have similar cerebellar development to AGA infants, those born at or after 34 weeks exhibited significantly poorer cerebellar development compared to their AGA counterparts.
With the increasing incidence of drug-resistant tuberculosis (DR-TB), determining a rapid and accurate drug susceptibility testing (DST) method to identify ethambutol (EMB) resistance in has become essential for patient management in China. Herein, we evaluated the correlation between three phenotypic DST methods, namely, proportion method (PM), MGIT 960 system, and microplate alamar Blue assay (MABA), and DNA sequencing of in 118 isolates from China. When the results of the phenotypic DST methods were compared with those of DNA sequencing, the overall agreement and kappa values of the PM, MGIT 960 system, and MABA were 81.
View Article and Find Full Text PDFAccuracy of a reverse dot blot hybridization assay for simultaneous detection of the resistance of four anti-tuberculosis drugs in Mycobacterium tuberculosis isolated from China.
Li Wan Qian Guo Jian-Hao Wei Hai-Can Liu Ma-Chao Li Li-Li Zhao Zhi-Guang Liu Kang-Lin Wan Gui-Lian Li
Infect Dis Poverty
April 2020
Article Synopsis
- Drug resistant tuberculosis is a major global health issue, and a new reverse dot blot hybridization (RDBH) assay was developed to detect resistance to four key anti-tuberculosis drugs in M. tuberculosis isolates from China.
- The study involved testing 320 clinical samples using the RDBH assay and comparing its accuracy against traditional drug susceptibility testing and sequencing methods.
- Results showed high concordance rates (up to 99%) and sensitivity (up to 97.9%) for the RDBH assay, indicating its potential as a fast, reliable method for identifying drug resistance in tuberculosis.
Article Synopsis
- * Results showed that 34.8% of the isolates were resistant to at least one of seven tested drugs, with multidrug-resistant and extensively drug-resistant tuberculosis rates at 17.0% and 1.4%, respectively.
- * Although drug resistance was more common in Beijing genotype strains, the study found no significant difference in drug resistance between the Beijing and non-Beijing genotypes, particularly among previously treated patients.
Article Synopsis
- This study investigated the potential of a new Mycobacterium tuberculosis antigen called Rv0674 for diagnosing and vaccinating against tuberculosis (TB).* -
- Results showed that TB patients had a stronger immune response (higher IgG levels) to Rv0674 compared to healthy controls, with decent sensitivity (77.1%) and specificity (81.1%) in serological tests.* -
- The research suggests Rv0674 could be a promising candidate for a TB diagnosis tool and vaccine, as it activates important immune responses in test subjects.*
Article Synopsis
- The study aimed to better understand macrolide susceptibility and resistance mechanisms in non-tuberculous mycobacteria (NTM) in China for improved infection treatment.
- Testing involved 310 clinical NTM isolates with four macrolides, revealing varied resistance patterns, particularly showing M. abscessus subsp. massiliense was mostly susceptible while M. fortuitum was highly resistant.
- Key resistance mutations were identified, but known mechanisms (23S rRNA and erm) did not explain all resistance cases, suggesting further research is needed to uncover additional resistance factors in different NTM species.
Article Synopsis
- * Milk was treated with NaOH and mycobacteria were isolated using L-J medium, followed by identification through PCR and gene analysis, revealing it to be Mycobacterium elephantis.
- * The identified strain showed resistance to some antibiotics while being sensitive to several others, marking the first report of M. elephantis isolation from cow milk in China.
Article Synopsis
- Genotyping and DNA sequencing of 235 M. tuberculosis isolates from China revealed specific mutations in the lysX gene that serve as markers for Beijing and modern Beijing strains.
- These findings were supported by analysis of 24 additional M. tuberculosis genomes from various countries.
- A new, efficient multiplex allele-specific PCR method was developed to quickly detect these mutations in lysX, successfully identifying all Beijing and modern Beijing strains in the tested samples.
Article Synopsis
- - We studied mutations in 140 multidrug-resistant tuberculosis (MDR-TB) strains from China, focusing on three genes: rpsL, rrs, and gidB, and found that 65.7% were resistant to streptomycin (STR), mainly from the Beijing strain.
- - The study showed a strong correlation between STR resistance and the Beijing family of tuberculosis (P=0.00) and highlighted that specific mutations in rpsL and rrs were common in STR-resistant isolates.
- - Detecting mutations in these genes had high effectiveness, with 94.6% sensitivity and 91.7% specificity, making them reliable indicators for STR resistance, which could aid the development of rapid
Article Synopsis
- * A study of 139 MDR-TB isolates in China revealed that 56.8% were resistant to EMB, with a significant portion (82.2%) belonging to the Beijing family, and that 79.1% of isolates had mutations in the embCAB operon.
- * The most frequent mutations were found in the embB gene, especially at codon 306, which closely correlated with EMB resistance; however, using embB306-497 DNA sequencing for detection was highly sensitive but only moderately
Objective: A PCR-reverse dot blot hybridization (RDBH) assay was developed for rapid detection of rpoB gene mutations in 'hot mutation region' of Mycobacterium tuberculosis (M. tuberculosis).
Methods: 12 oligonucleotide probes based on the wild-type and mutant genotype rpoB sequences of M.
Article Synopsis
- The study focuses on extensively drug-resistant tuberculosis (XDR-TB) in China, highlighting the urgent need for effective TB control.
- Researchers sequenced 58 XDR-TB isolates to understand the correlation between drug resistance mutations and different genotype strains, achieving high specificity for several drugs like isoniazid and rifampicin.
- Findings indicate that the Beijing genotype strain is predominant among the isolates, with certain mutations linked to specific genetic lineages, paving the way for improved diagnostic techniques for XDR-TB.
Article Synopsis
- * Results showed varying levels of resistance to several drugs, including isoniazid (35.7%) and rifampin (26.9%), with a higher risk of drug resistance found in previously treated patients.
- * Most resistant isolates belonged to the Beijing family, with key mutations identified in specific genes, but no clear link was found between drug resistance and bacterial genotype, enhancing understanding of TB resistance in the region.
Article Synopsis
- X-ray crystallography was used to identify changes in the structure of a metal-organic framework.
- These changes involved solid-state transformations, which are alterations in the solid structure without melting or dissolving.
- The study highlights how precise techniques like X-ray crystallography can provide insights into the behavior of complex materials.
Molecular characterization of multidrug-resistant Mycobacterium tuberculosis isolates from China.
Antimicrob Agents Chemother
November 2014
Article Synopsis
- - The study focused on analyzing drug-resistant tuberculosis (MDR-TB) isolates from China, specifically looking at genetic mutations linked to resistance and their association with different strain types.
- - Out of 128 MDR-TB isolates, 84.4% were identified as belonging to the Beijing family, with 59.3% being modern strains, and specific mutations strongly correlated with resistance to various drugs like isoniazid and rifampin.
- - The research suggests that while the DNA sequencing methods used showed high sensitivity and specificity for detecting drug resistance, there was no direct link between certain mutations and the strain's genetic makeup, aiding in the development of faster diagnostic tests in China.
Objective: To detect the changes on the expression of putative drug efflux genes caused by isoniazid-inducement in single resistance to the isoniazid Mycobacterium tuberculosis (M. tuberculosis) clinical isolates, for exploring the putative efflux genes which causing M. tuberculosis isoniazid resistance as well as the mechanism related to high expression of the putative efflux genes.
View Article and Find Full Text PDFArticle Synopsis
- The study aimed to analyze the distribution and characteristics of Mycobacterium tuberculosis genotypes in Tianjin and their link to drug resistance.
- Researchers collected 656 clinical strains and tested their resistance to four anti-tuberculosis drugs, while also identifying genotypes using multiplex PCR.
- Results indicated that the Beijing genotype was prevalent among the strains, found in 91.46% of isolates, but there was no significant correlation between this genotype and drug resistance patterns.
Article Synopsis
- Tuberculosis is a major public health concern, particularly the drug-resistant Beijing strains prevalent in East Asia, prompting a study on mutation patterns in Tianjin, China.
- Researchers analyzed 822 M. tuberculosis isolates, identifying 169 drug-resistant cases and examining mutations in key genes associated with resistance to various antibiotics like rifampin and isoniazid.
- The study revealed significant mutations in specific resistance-determining regions, highlighting patterns that enhance the understanding of drug resistance in these strains and could inform treatment strategies.
[Clinical feature analysis of 190 patients with anti-neutrophil cytoplasmic antibodies associated vasculitis].
Zhonghua Yi Xue Za Zhi
September 2009
Article Synopsis
- The study focused on understanding the different clinical features and disease characteristics of ANCA-associated vasculitis (AAV) by reviewing 190 patient cases from 1998 to 2008.
- Patients were divided into two groups based on ANCA test results: cytoplasmic ANCA (C-ANCA) and perinuclear ANCA (P-ANCA), revealing differences in symptoms and organ involvement.
- The findings indicated that P-ANCA patients had higher mortality rates and were associated with respiratory and organ failures, while C-ANCA patients had more gastrointestinal and joint symptoms.