[Taxonomic characteristics and antibiotic susceptibility of microorganisms verified in the expressed prostate secretion in post-COVID-19 patients with recurrent chronic bacterial prostatitis].

Introduction: Recurrent chronic bacterial prostatitis (rCBP) is a hard-to diagnosis-and-treat disease which there is no consensus. A particularly difficult cohort is represented by patients who had COVID-19. The study aimed to evaluate the taxonomic structure and sensitivity to antibacterial drugs of microorganisms verified in expressed prostate secretion (EPS) in rCBP-patients who had COVID-19.

[The role of electron microscopy of ejaculate in the diagnosis of infertility associated with human papillomavirus infection].

Introduction: The problem of male infertility is multifactorial. However, in recent years, the question of the involvement of viruses, in particular human papillomaviruses (HPV), in the development of this condition has been actively discussed.

Purpose Of The Study: To study the role of ejaculate electron microscopy in the diagnosis of infertility associated with human papillomavirus infection.

[Extended culture study as a keypoint to rethinking antibiotic therapy for chronic bacterial prostatitis].

Objective: To juxtapose the microbiological efficacy of standard and targeted antibiotic therapy (ABT) based on the comparison of the results of extended bacteriology of biomaterial in patients suffering chronic bacterial prostatitis (CBP) before and after treatment.

Study Design: single-centre observational comparative study. Sixty patients with CBP aged 20 to 45 years were included in the study.

[Asymptomatic bacteriuria in pregnant women the normal condition of healthy women urine].

Objective: to study the dynamics of the microbiota of a freshly excreted middle portion of urine in primigravida at different times of all three trimesters of pregnancy.

Materials And Methods: A single-center prospective observational cohort study was conducted with a consecutive enrollment of 30 women at different gestational ages: I - 8-12 weeks, II - 22-24 weeks, III - 30-32 weeks. A midstream specimen of morning vesical urine was taken for the study, then it was cultivated using nutrient media for aerobic and anaerobic microorganisms under appropriate conditions.

[Long-term inflammatory and neoplastic reaction of prostate tissues during its transurethral infection with uropathogens: evaluation of the results of animal model study].

Introduction: There is no convincing evidence of the persistence of acute or the development of chronic bacterial-induced prostatic inflammation in the long term when infected with various titers of the uropathogen. Along with this, controversial data are presented on the relationship between post-infectious chronic inflammation and neoplastic changes in prostate tissues.

Objective: To carry out, based on the experimental data: 1) assessment of the degree of bacterial contamination and the severity of histological changes in prostate tissues on the 60th follow-up day in case of transurethral infection with various uropathogens in titers of 102,3,5 CFU/ml; 2) fundamental comparative analysis between the indicators of the inoculated test-titer and microbial load with the severity of histological changes in prostate tissues; 3) verification of neoplastic transformations in the prostate tissues during a long-term persistent bacterial-induced inflammatory process.

Does the microbiota spectrum of prostate secretion affect the clinical status of patients with chronic bacterial prostatitis?

Objective: To explore the influence of the microbiota of prostate secretion on the clinical status of patients with chronic bacterial prostatitis.

Methods: This was an observational, single-center, comparative study. We evaluated the survey cards of 230 outpatients aged 18-45 years with a history of prostatitis from 2012 to 2019.

The gene profile of virulence factors in relation to bacteriuria levels between the acute episodes of recurrent uncomplicated lower urinary tract infection.

Objectives: The pathogenic potential of uropathogens isolated between acute episodes of recurrent lower urinary tract infection (rLUTI) is studied insufficiently. The objectives were to determine the spectrum of virulence genes of Enterobacteriaceae cultured between acute episodes of rLUTI at various levels of bacteriuria.

Methods: Bacteriological examinations of 169 premenopausal women's midstream urine with rLUTI were performed between acute episodes of UTI.

Does Escherichia coli have pathogenic potential at a low level of bacteriuria in recurrent, uncomplicated urinary tract infection?

Background: In recurrent uncomplicated lower urinary tract infection (uLUTI), bacteriuria below 10 colony-forming units (CFU)/mL is not usually investigated.

Objective: To determine the molecular-genetic characteristics of the pathogenic potential of Enterobacteriaceae isolated between episodes of recurrent uLUTI at bacteriuria of 10 CFU/mL.

Materials And Methods: Midstream urine samples taken from 169 women between episodes of recurrent uLUTI were examined on six media.

[Virusuria as a component of the urine microbiota and its significance for assessing the health of the urinary tract: a descriptive clinical study].

Aim: To determine the frequency of occurrence of oportunistic pathogenic bacterial flora and viral pathogens in the urine of healthy people with the establishment of the association between them.

Materials And Methods: 40 healthy sexually active women and men were examined, which are divided by gender into equivalent groups: Group I - healthy women (n=19), Group II - healthy men (n=21). The age of the subjects ranged from 20 to 25 years, the average age was 22.

[Long-term monitoring of leukocyturia and bacteriuria after acute obstructive pyelonephritis].

The generally accepted standard of antibiotic therapy aimed at causative agent does not exclude the risk of acute obstructive pyelonephritis (AOP) to become chronic after the end of antibiotic therapy, resulting in a high incidence of relapses. To assess the outcomes of the AOP, we undertook a prospective clinical trial. MATERIALS AND METHODS: The study comprised 51 consistently selected patients (12 men and 39 women) with AOP occurred as a result of unilateral ureteric calculus obstruction.

[Bacterial prostatitis: epidemiology and etiology].

Prostatitis is considered as heterogeneous group of the diseases attracting broad interest of researchers worldwide. The acute and chronic stages of the process, as particular categories of bacterial prostatitis, remain in the realities of modern practical and scientific urology with lively discussions. Despite a large number of domestic and international publications, consensus on many topical issues of bacterial prostatitis is currently not achieved.

[Antimicrobial resistance of uropathogens in patients with acute obstructive pyelonephritis].

Relevance: Acute pyelonephritis is known to be the most complicated and severe urinary tract infection occurring in all age groups and accounting for 14% of all kidney diseases. The generally recognized standard antibiotic therapy cannot completely prevent the progression of the disease to its chronic form after relief of its acute manifestations thus leading to a high incidence of relapses. The aim of our study was to investigate the spectrum of uropathogens and their antibiotic sensitivity in acute obstructive pyelonephritis.

[Is bacteriological testing of bladder urine informative in acute obstructive pyelo- nephritis?].

Unlabelled: The problem of the etiology and pathogenesis of acute obstructive pyelonephritis (OOP) remains one of the challenging issues of modern urology. Etiological agents of pyelonephritis can be both gram-negative and gram-positive opportunistic bacteria mostly belonging to the normal flora in humans. The generally accepted diagnostic work-up involves a bacteriological testing of not pelvic urine, but of bladder urine collected by a transurethral catheter or midstream specimens of urine collected from the patients.

[Experimental modeling of acute pyelonephritis].

Acute pyelonephritis (AP) predominates among inflammatory infection kidney affections. In accordance to international classifications, AP is an upper urinary tract infection, subdivided into non-complicated (non-obstructive) and complicated (obstructive) forms. The clinical significance of AP presently is defined by the conditions frequent occurrence and its progression into chronic kidney disease.

[Microbiota of urine and vagina of healthy postmenopausal women (a pilot study)].

Studying microbiota of different urogenital tract habitats in healthy postmenopausal women is of practical importance in deciding on the appropriateness of correction of dysbiotic disorders. The aim of this study was to examine the vaginal and urine microbiota of healthy postmenopausal women. The study included 20 healthy postmenopausal women (mean age 59,0+/-2,1 years).

[Microbiota of lower urine tract and genital organs of healthy men and in infertility].

Aim: Study microflora of urine, ejaculate, urethra scrape in normal state and infertility.

Materials And Methods: 2 groups of men were examined: I (28)--control, conditionally healthy men (20 - 25 years of age), II (26)--infertile patients (25 - 35 years of age). Middle portion of morning urine, ejaculate, urethra scrape were studied in group I, in II--ejaculate.

[Unsterile urine in health human--new paradigm in medicine].

The 3-fold urine culture evaluation in healthy women (24) and men (28) was performed. In 100% of cases, various types of multicomponent aerobic-anaerobic microorganism associations were found. Dominant clusters in the urine of women incleded coagulase-negative staphylococci, Corynebacterium sp.

Human urine is not sterile - shift of paradigm.

Objective: Until recently the generally accepted paradigm implied that urine of healthy people is sterile. In the present study, urine of healthy subjects was investigated by extended bacteriological methods.

Material And Methods: Three midstream urine samples from 52 healthy subjects each (24 females, 28 males; 18-25 years of age) were investigated by an extended set of culture media for identification of facultative aerobic (FAB) and nonclostridial anaerobic bacteria (NCAB).

[The microbial spectrum of urine and bladder bioptic samples in women with chronic recurrent cystitis].

The evaluation of urine and bladder bioptic samples (bacteriological examination and polymerase chain reaction) of 28 women with chronic cystitis was performed. In 85.7% of cases, mixed infections with predominance of nonclostridial anaerobes in the urine was detected.

[Non-clostridial anaerobic bacteria can cause acute pyelonephritis (an experimental trial)].

Acute obstructive pyelonephritis (PN) caused by Peptococcus niger (Pn), E. coli and mixed infection (Pn + E. coli) was modeled in rabbits.

[Etiological structure and antibiotic sensitivity of uropathogens in chronic recurrent infection of the lower urinary tract].

While acute infections of the lower urinary tract (UT) have been studied in detail and antibiotic therapy of such infections is well known, etiology and choice of antibacterial treatment in recurrent chronic UT infection are not so clear. In our trial we aimed at elucidation of etiological structure of chronic cystitis recurrences by means of microbiological investigation of urine samples from 72 women on extended spectrum of nutrient media. In all the cases urine was infected with both aerobic and non-clostridial anaerobic bacteria.

[Bacterial mixed infection in women with chronic recurrent cystitis].

Aim: To study microbial repertoire of urine in healthy women and patients with chronic recurrent cystitis (CRC) including facultative anaerobic (FA) and non-clostridial anaerobic (NCA) bacteria. MATERIALS AND METHODS. Triple bacteriological study of urine was performed in three groups of women: group I--22 healthy virgin women aged 18- 25 years, group II--24 women aged 18 - 25 years with regular sexual contacts, group III--72 women aged 20 - 60 years with CRC, before antibacterial therapy.

[Bacterial spectrum of the urine in young healthy women].

A wide spectrum of aerobic-anaerobic associations were found at bacteriological examination of 138 samples of the middle urine portion from 46 healthy women aged 17-25 years (22 sexually inactive and 24 active). Facultative-anaerobic (corinebacteria and coagulase-negative staphylococci) and non-clostridial anaerobic bacteria (peptococci and propionibacteria) predominated in both groups. The majority of facultative anaerobic bacteria had marked adhesive and antilysosyme activity indicating a pathogenic potential.