Impacts of Black Soldier Fly () Larval Meal on Intestinal Histopathology and Microbiome Responses in Hybrid Grouper ( ♀ × ♂): A Comprehensive Analysis.

This study examined the diversity and responses of intestinal microbiota in hybrid grouper ( ♀ × ♂) fed diets with varying levels of fishmeal replaced by black soldier fly larvae (BSFL). The 10% BSFL substitution (BSFL10) group showed the highest levels of trypsin and amylase. Substituting fishmeal with 30% and 50% BSFL weakened the intestinal wall, resulting in vacuoles, sparse striatal boundaries, and fewer villi.