Clinicopathological features of Lynch syndrome pedigrees with MSH2 c.351G>A gene variant.

Background: Lynch syndrome (LS) is an autosomal-dominant disorder that increases the risk of many cancers. To identify novel or rare pathogenic variants of MMR genes associated with LS, especially in Chinese pedigrees.

Methods: One four-generation Chinese Han family from northeast China with 29 members was enrolled.

Dihydroxanthene-Based Near-infrared Fluorescent Probes for Monitoring Mitochondrial Viscosity in Living Cells and Mice.

Aberrant mitochondrial viscosity is closely associated with many diseases and cellular malfunctions. Thus, the development of reliable methods for monitoring mitochondrial viscosity variations has attracted considerable attention. Herein, through stepwise structural modulation of the dihydroxanthene fluorophore (), we developed three NIR fluorescent probes, named , for detecting mitochondrial viscosity.

Screening of dicyanoisophorone-based probes for highly sensitive detection of viscosity changes in living cells and zebrafish.

Herein, seven viscosity-sensitive probes were developed simple structural modification of dicyanoisophorone (DCO)-derived dyes. Among them, DCO-5 significantly enhances (180-fold) the response signal in highly viscous aqueous media while showing insensitivity to polarity changes or pH variations, and enables the successful detection of viscosity changes in nystatin-treated HepG2 cells, PC 12 cells and zebrafish.