Antibodies from dengue patients with prior exposure to Japanese encephalitis virus are broadly neutralizing against Zika virus.

Exposure to multiple mosquito-borne flaviviruses within a lifetime is not uncommon; however, how sequential exposures to different flaviviruses shape the cross-reactive humoral response against an antigen from a different serocomplex has yet to be explored. Here, we report that dengue-infected individuals initially primed with the Japanese encephalitis virus (JEV) showed broad, highly neutralizing potencies against Zika virus (ZIKV). We also identified a rare class of ZIKV-cross-reactive human monoclonal antibodies with increased somatic hypermutation and broad neutralization against multiple flaviviruses.

Diagnostic value and role of serum miR-15a-5p in patients with schizophrenia.

Background: More and more studies have confirmed that the heredity plays an important role in mental disorders, especially microRNA. The objective of this research was to explore the level of miR-15a-5p in patients with schizophrenia (SZ), and to evaluate the feasibility of this miRNA as a diagnostic marker of SZ.

Methods: The serum level of miR-15a-5p in patients with SZ and healthy people was detected by RT-qPCR.

Experimental and Numerical Investigation on Pile Foundation Underpinning Structure System in Urban Overpass.

In view of the complexity of the pile foundation underpinning structure system and the stringent requirements of the construction process, this paper briefly describes the necessity of introducing epoxy resin reinforcing adhesive of planting rebar in the design of pile foundation underpinning beam structure to improve the mechanical properties of the reinforced beam new and old concrete joint surfaces and proposes a new type of pile foundation replacement beam system construction method by "chiseling + prestressed reinforcement + epoxy resin reinforcing adhesive". This paper uses an actual pile foundation underpinning project of an urban overpass as a prototype, designs and creates a model structure with a similarity ratio of 1/6, and performs repeated progressive static loading tests to study the load carrying capacity, displacement change, and other properties of the strengthened replacement structure, as well as analyses and distorts the overall working performance and failure mode of them. On this basis, the prototype structure's finite element analysis model was built, and the finite element analysis results were compared with the test results to obtain the mechanical properties and deformation characters of the actual pile foundation underpinning structure system corresponding to the actual underpinning beam load.

Investigation of LncRNA PVT1 and MiR-21-5p Expression as Promising Novel Biomarkers for Autism Spectrum Disorder.

The characteristics of ncRNA in children with autism spectrum disorder (ASD) were observed to disclose a theoretical basis for further research on molecular markers for early warning of ASD. Children with ASD and normal control children were recruited to collect peripheral blood RNA samples. The concentration of PVT1 and miR-21-5p was quantitatively analyzed by qRT-PCR.

Collagen-binding peptides for the enhanced imaging, lubrication and regeneration of osteoarthritic articular cartilage.

Treatments for osteoarthritis would benefit from the enhanced visualization of injured articular cartilage and from the targeted delivery of disease-modifying drugs to it. Here, by using ex vivo human osteoarthritic cartilage and live rats and minipigs with induced osteoarthritis, we report the application of collagen-binding peptides, identified via phage display, that are home to osteoarthritic cartilage and that can be detected via magnetic resonance imaging when conjugated with a superparamagnetic iron oxide. Compared with the use of peptides with a scrambled sequence, hyaluronic acid conjugated with the collagen-binding peptides displayed enhanced retention in osteoarthritic cartilage and better lubricated human osteoarthritic tissue ex vivo.

State-of-Charge Estimation for Lithium-Ion Batteries Using Residual Convolutional Neural Networks.

State-of-charge (SOC) is a relative quantity that describes the ratio of the remaining capacity to the present maximum available capacity. Accurate SOC estimation is essential for a battery-management system. In addition to informing the user of the expected usage until the next recharge, it is crucial for improving the utilization efficiency and service life of the battery.

Differential Proteomic Analysis of during High-Pressure Processing.

High-pressure processing (HPP) is a prevailing non-thermal food preservation technology. The inactivation mechanisms of under HPP at 200 and 400 MPa for 3 min were investigated by label-free quantitative proteomic analysis and functional enrichment analysis in the Kyoto Encyclopedia of Genes and Genomes. HPP treatment at 400 MPa exhibited significant effects on proteins involved in translation, carbon, carbohydrate, lipid and energy metabolism, and peptidoglycan biosynthesis.

Combined impact of high-pressure processing and slightly acidic electrolysed water on Listeria monocytogenes proteomes.

Slightly acidic electrolysed water (SAEW) and high-pressure processing (HPP) are well-established non-thermal preservation technologies. This study investigated the deactivation mechanisms of Listeria monocytogenes by label-free quantitative proteomics analysis. Samples were treated through HPP (300 MPa for 3 min), SAEW (20 ppm available chlorine concentration), and their combinations.

Purification and Identification of Cholesterol Micelle Formation Inhibitory Peptides of Hydrolysate from High Hydrostatic Pressure-Assisted Protease Hydrolysis of Fermented Seabass Byproduct.

This research focuses on the proteolytic capacity of sea bass byproduct (SB) and their hypocholesterolemic activity via the cholesterol micelle formation (CMF) inhibition. SB was fermented with seven mixed lactic acid bacteria for 5 h at 42 °C. The lactic fermented SB was hydrolyzed with Protease N for 6 h under HHP to obtain the SB hydrolysates (HHP-assisted Protease N hydrolysis after fermentation, F-HHP-PN6).

Anti-Fatigue Effect of a Dietary Supplement from the Fermented By-Products of Taiwan Tilapia Aquatic Waste and Oligosaccharide Complex.

The Taiwan Tilapia is an important aquaculture product in Taiwan. The aquatic by-products generated during Tilapia processing, such as fish bones and skin, are rich in minerals and protein. We aimed to explore the effect of a dietary supplement, comprising a mixture of fermented Tilapia by-products and oligosaccharides as the raw materials, combined with physical training on exercise performance and fatigue.

Fabrication of novel AgBiO towards excellent photocatalytic oxidation of gaseous toluene under visible light irradiation.

In this work, a novel oxide combined with bismuth (Bi) and silver (Ag) was prepared via simple ball milling. This substance was optimized by adjusting the amount of pre-source. Preliminary characterization results confirmed the successful synthesis of AgBiO.

The influence of solar natural heating and NO-O photochemistry on flow and reactive pollutant exposure in 2D street canyons.

This study incorporates solar radiation model and NO-O photochemistry into computational fluid dynamics (CFD) simulations with the standard k-ε model to quantify the integrated impacts of turbulent mixing, solar heating and chemical processes on vehicular passive (CO) and reactive (NO, O) pollutant dispersion within two-dimensional (2D) street canyons. Various street aspect ratios (H/W = 1, 3, 5) and solar-radiative scenarios (LST 0900, 1200, 1500) are considered. The initial source ratio of NO to NO is 1:10 and the background O concentration is 100 ppb (mole fraction).

Integrated impacts of tree planting and aspect ratios on thermal environment in street canyons by scaled outdoor experiments.

Urban tree planting has the potential to reduce urban heat island intensity and building energy consumption. However, the heterogeneity of cities makes it difficult to quantitatively assess the integrated impacts of tree planting and street layouts. Scaled outdoor experiments were conducted to investigate the influence of tree plantings on wind and thermal environments in two-dimensional (2D) north-south oriented street canyons with various aspect ratios (building height/street width, AR = H/W = 1, 2, 3; H = 1.

Scaled outdoor experimental studies of urban thermal environment in street canyon models with various aspect ratios and thermal storage.

Street aspect ratios and urban thermal storage largely determine the thermal environment in cities. By performing scaled outdoor measurements in summer of 2017 in Guangzhou, China, we investigate these impacts on spatial/temporal characteristics of urban thermal environment which are still unclear so far. Two types of street canyon models are investigated, i.

Integrated impacts of turbulent mixing and NO-O photochemistry on reactive pollutant dispersion and intake fraction in shallow and deep street canyons.

We employ computational fluid dynamics (CFD) simulations with NO-NO-O chemistry to investigate the impacts of aspect ratios (H/W = 1,3,5), elevated-building design, wind catchers and two background ozone concentrations ([O] = 100/20 ppb) on reactive pollutant dispersion in two-dimensional (2D) street canyons. Personal intake fraction of NO (P_IF) and its spatial mean value in entire street (i.e.

Differential effect of herbal tea extracts on free fatty acids-, ethanol- and acetaminophen-induced hepatotoxicity in FL83B hepatocytes.

In recent years, herbal tea consumption becomes popular because of the potential health benefits and attractive flavors. However, there is also a growing concern that herbal supplements contribute to the drug-drug/drug-herb interactions and hepatotoxicity. In this study, FL83B mouse hepatocytes were used as an mode of hepatotoxicity induced by free fatty acids, including palmitic acid (PA) and oleic acid (OA), ethanol, and acetaminophen.

Preparation and Identification of Novel Antihypertensive Peptides from the In Vitro Gastrointestinal Digestion of Marine Cobia Skin Hydrolysates.

This research focuses on cobia skin hydrolysates and their antihypertensive effects via the inhibitory activities of angiotensin I-converting enzyme (ACE). Marine fish Cobia () skin was hydrolysed for 5 h using Protamex and Protease N to obtain the cobia skin protein hydrolysates PX-5 and PN-5, respectively. The soluble protein and peptide contents of the PX-5 were 612 and 270 mg/g, respectively, and for the PN-5, 531 and 400 mg/g, respectively.

Effect of Chitosan and Fish Gelatin Coatings on Preventing the Deterioration and Preserving the Quality of Fresh-Cut Apples.

The effect of fish gelatin and chitosan coatings on the physicochemical characteristics of fresh-cut apples ( Mill.), stored at 5 °C and 22 °C, was investigated. Chitosan provided an effective control for microbial growth, maintained firmness during 4 days of storage at room temperature (22 °C), and 12 days at refrigerator (5 °C).

Numerical evaluations of urban design technique to reduce vehicular personal intake fraction in deep street canyons.

High-rise deep street canyons usually experience poor ventilation and large vehicular pollutant exposure to residents in near-road buildings. Investigations are still required to clarify the flow and dispersion mechanisms in deep street canyons and explore techniques to reduce such large pollutant exposure. By conducting computational fluid dynamics (CFD) simulations validated by wind tunnel data and scale-model outdoor field measurements, we investigate the integrated impacts of aspect ratios, first-floor and second-floor elevated building designs, viaduct settings, height variations and wind catchers on the flow, personal intake fraction (P_IF) of CO (carbon dioxide) and its spatial mean value 〈P_IF〉 in two-dimensional (2D) street canyons.

Purification and Identification of Angiotensin I-Converting Enzyme Inhibitory Peptides and the Antihypertensive Effect of Chlorella sorokiniana Protein Hydrolysates.

Hot water was used to obtain hot water extract (HWE). Subsequently, this byproduct was freeze-dried, hydrolysed at 50 °C using Protease N to obtain protein hydrolysates (PN-1), and then digested with a gastrointestinal enzyme (PN-1G). The inhibitory effects of the HWE and hydrolysates against angiotensin I-converting enzyme (ACE) were investigated.

Antibacterial Activity of Emulsified Pomelo (Citrus grandis Osbeck) Peel Oil and Water-Soluble Chitosan on Staphylococcus aureus and Escherichia coli.

This study utilized pomelo steam distillation to isolate pomelo peel essential oil. The constituents were then analyzed through gas chromatography-mass spectrometry (GC-MS), and the antibacterial activity of the essential oil emulsions at different homogenizer speed conditions and concentrations of water-soluble chitosan (degree of acetylation, DA = 54.8%) against and was examined.

Carbon black aggregates cause endothelial dysfunction by activating ROCK.

Carbon black nanoparticles (CBNs) have been associated with the progression of atherosclerosis. CBNs normally enter the bloodstream and crosslink together to form agglomerates. However, most studies have used nano-sized CB particles to clarify the involvement of CBN exposure in CBN-induced endothelial dysfunction.

Improved Anticancer Photothermal Therapy Using the Bystander Effect Enhanced by Antiarrhythmic Peptide Conjugated Dopamine-Modified Reduced Graphene Oxide Nanocomposite.

Despite tremendous efforts toward developing novel near-infrared (NIR)-absorbing nanomaterials, improvement in therapeutic efficiency remains a formidable challenge in photothermal cancer therapy. This study aims to synthesize a specific peptide conjugated polydopamine-modified reduced graphene oxide (pDA/rGO) nanocomposite that promotes the bystander effect to facilitate cancer treatment using NIR-activated photothermal therapy. To prepare a nanoplatform capable of promoting the bystander effect in cancer cells, we immobilized antiarrhythmic peptide 10 (AAP10) on the surface of dopamine-modified rGO (AAP10-pDA/rGO).