Development of a novel anti-erythropoietin-producing hepatocellular receptor B6 monoclonal antibody EbMab-3 for flow cytometry.

Erythropoietin-producing hepatocellular receptor B6 (EphB6) is a member of the largest Eph subfamily of receptor tyrosine kinases. EphB6 is widely expressed in various tissues and regulates cellular homeostasis by interacting with its membrane-bound ephrin ligands and other receptors. EphB6 is involved in cancer pathology despite lacking kinase activity.

Downregulation of NAD Kinase Expression in β-Cells Contributes to the Aging-Associated Decline in Glucose-Stimulated Insulin Secretion.

Nicotinamide adenine dinucleotide kinase (NADK) is essential to the generation of nicotinamide adenine dinucleotide phosphate (NADP(H)), an important metabolic coupling factor involved in glucose-stimulated insulin secretion. In the present study, we showed that the expression of Nadk and Nadk2 transcripts and NADP(H) content were lower in islets of 80-week-old (aged) mice than those of 8-week-old (young) mice. This was associated with diminished oral glucose tolerance of old mice and the glucose-stimulated insulin secretion (GSIS) response of islets.

A novel anti-mouse CCR7 monoclonal antibody, CMab-7, demonstrates high sensitivity in flow cytometry, western blot, and immunohistochemistry.

C-C chemokine receptor type 7 (CCR7) is a member of the G protein-coupled receptor family and functions as a lymph node-homing receptor for immune cells. Upon ligand binding, CCR7 promotes the migration of immune cells to secondary lymphoid organs. In cancers, CCR7 has been revealed as a critical molecule in lymph node metastasis.

Driving Intention Recognition of Electric Wheel Loader Based on Fuzzy Control.

Energy conservation and emission reduction is a common concern in various industries. The construction process of electric wheel loaders has the advantages of being zero-emission and having a high energy efficiency, and has been widely recognized by the industry. The frequent shift in wheel loader working processes poses a serious challenge to the operator.

Clinical Relevance and Drug Modulation of PPAR Signaling Pathway in Triple-Negative Breast Cancer: A Comprehensive Analysis.

Triple-negative breast cancer (TNBC) is highly heterogeneous and poses a significant medical challenge due to limited treatment options and poor outcomes. Peroxisome proliferator-activated receptors (PPARs) play a crucial role in regulating metabolism and cell fate. While the association between PPAR signal and human cancers has been a topic of concern, its specific relationship with TNBC remains unclear.

Functional Hydrogels for Aqueous Zinc-Based Batteries: Progress and Perspectives.

Aqueous zinc batteries (AZBs) hold great potential for green grid-scale energy storage due to their affordability, resource abundance, safety, and environmental friendliness. However, their practical deployment is hindered by challenges related to the electrode, electrolyte, and interface. Functional hydrogels offer a promising solution to address such challenges owing to their broad electrochemical window, tunable structures, and pressure-responsive mechanical properties.

Reviving Zn Dendrites to Electroactive Zn by Ion Sieve Interface.

Zn dendrite formation during repeated plating/stripping processes limits the practical use of Zn-metal anodes in reliable and affordable energy storage. Traditional methods, including dendrite suppression and dendrite regulation, fail under demanding performance conditions due to Zn diffusion limitations and concentration gradients. Here, using an in situ pre-zincation approach, a LiZnTiO (LZTO, 0 View Article and Find Full Text PDF

High-Entropy Alloys in Catalysis: Progress, Challenges, and Prospects.

High-entropy alloys (HEAs) have become pivotal materials in the field of catalysis, offering unique advantages due to their diverse elemental compositions and complex atomic structures. Recent advances in computational techniques, particularly density functional theory (DFT) and machine learning (ML), have significantly enhanced our understanding and design of HEAs for use in catalysis. These innovative atomistic simulations shed light on the properties of HEAs, enabling the discovery and optimization of catalysis materials for solid-solution structures.

construction of a static-dynamic hybrid interface toward stable Zn anodes for aqueous Zn-ion batteries.

Aqueous Zn-ion batteries are promising candidates for next-generation energy storage devices due to the advantages of high safety, low cost and good environmental friendliness. However, the uncontrollable dendrite growth and undesirable side reactions occurring on the Zn anode result in poor cycling stability. Herein, a Lewis base, triethanolamine, is used as the electrolyte additive to construct a hybrid solid-electrolyte interphase layer composed of a static ZnSO·3Zn(OH)·4HO layer and dynamic quaternary ammonium ion adsorption layer.

Anti-CD44 Variant 10 Monoclonal Antibody Exerts Antitumor Activity in Mouse Xenograft Models of Oral Squamous Cell Carcinomas.

CD44 regulates cell adhesion, proliferation, survival, and stemness and has been considered a tumor therapy target. CD44 possesses the shortest CD44 standard (CD44s) and a variety of CD44 variant (CD44v) isoforms. Since the expression of CD44v is restricted in epithelial cells and carcinomas compared to CD44s, CD44v has been considered a promising target for monoclonal antibody (mAb) therapy.

Antitumor activities of anti‑CD44 monoclonal antibodies in mouse xenograft models of esophageal cancer.

CD44 is a type I transmembrane glycoprotein associated with poor prognosis in various solid tumors. Since CD44 plays a critical role in tumor development by regulating cell adhesion, survival, proliferation and stemness, it has been considered a target for tumor therapy. Anti‑CD44 monoclonal antibodies (mAbs) have been developed and applied to antibody‑drug conjugates and chimeric antigen receptor‑T cell therapy.

Development of Sensitive Anti-Mouse CCR5 Monoclonal Antibodies Using the N-Terminal Peptide Immunization.

One of the G protein-coupled receptors, C-C chemokine receptor 5 (CCR5), is an important regulator for the activation of T and B lymphocytes, dendritic cells, natural killer cells, and macrophages. Upon binding to its ligands, CCR5 activates downstream signaling, which is an important regulator in the innate and adaptive immune response through the promotion of lymphocyte migration and the secretion of proinflammatory cytokines. Anti-CCR5 monoclonal antibodies (mAbs) have been developed and evaluated in clinical trials for tumors and inflammatory diseases.

Development of a Sensitive Anti-Mouse CCR5 Monoclonal Antibody for Flow Cytometry.

C-C chemokine receptor 5 (CCR5), a member of the G protein-coupled receptor family, is the most common coreceptor for the human immunodeficiency virus type 1. CCR5 is also involved in the pathogenesis of tumors and inflammatory diseases. The CCR5 antagonists including monoclonal antibodies (mAbs) have been developed and evaluated in clinical trials.

PMab-314: An Anti-Giant Panda Podoplanin Monoclonal Antibody.

The giant panda () is one of the important species in worldwide animal conservation. Because it is essential to understand the disease of giant panda for conservation, histopathological analyses of tissues are important to understand the pathogenesis. However, monoclonal antibodies (mAbs) against giant panda-derived proteins are limited.

CxMab-1: A Novel Anti-mouse CXCR1 Monoclonal Antibody for Flow Cytometry.

The C-X-C motif chemokine receptor-1 (CXCR1) is a rhodopsin-like G-protein-coupled receptor, expressed on the cell surface of immune cells and tumors. CXCR1 interacts with some C-X-C chemokines, such as CXCL6, CXCL7, and CXCL8/interleukin-8, which are produced by various cells. Since CXCR1 is involved in several diseases including tumors and diabetes mellitus, drugs targeting CXCR1 have been developed.

High Entropy Alloys Enable Durable and Efficient Lithium-Mediated CO Redox Reactions.

Designing electrocatalysts with high activity and durability for multistep reduction and oxidation reactions is challenging. High-entropy alloys (HEAs) are intriguing due to their tunable geometric and electronic structure through entropy effects. However, understanding the origin of their exceptional performance and identifying active centers is hindered by the diverse microenvironment in HEAs.

Bismuth-doping boosting Na diffusion kinetics of layered oxide cathode with radially oriented {010} active lattice facet for sodium-ion batteries.

O3-type layered oxide cathodes (NaTMO) for sodium-ion batteries (SIBs) have attracted significant attention as one of the most promising potential candidates for practical energy storage applications. The poor Na diffusion kinetics is, however, one of the major obstacles to advancing large-scale practical application. Herein, we report bismuth-doped O3-NaNiMnO (NMB) microspheres consisting of unique primary nanoplatelets with the radially oriented {010} active lattice facets.

Progress and perspectives on iron-based electrode materials for alkali metal-ion batteries: a critical review.

Iron-based materials with significant physicochemical properties, including high theoretical capacity, low cost and mechanical and thermal stability, have attracted research attention as electrode materials for alkali metal-ion batteries (AMIBs). However, practical implementation of some iron-based materials is impeded by their poor conductivity, large volume change, and irreversible phase transition during electrochemical reactions. In this review we critically assess advances in the chemical synthesis and structural design, together with modification strategies, of iron-based compounds for AMIBs, to obviate these issues.

Rational Design of Multinary Metal Chalcogenide BiSbTe Nanocrystals for Efficient Potassium Storage.

Multinary metal chalcogenides hold considerable promise for high-energy potassium storage due to their numerous redox reactions. However, challenges arise from issues such as volume expansion and sluggish kinetics. Here, a design featuring a layered ternary BiSbTe anchored on graphene layers as a composite anode, where Bi atoms act as a lattice softening agent on Sb, is presented.

Interfacial Engineering of Zn Metal via a Localized Conjugated Layer for Highly Reversible Aqueous Zinc Ion Battery.

Aqueous zinc-ion batteries are regarded as promising and efficient energy storage systems owing to remarkable safety and satisfactory capacity. Nevertheless, the instability of zinc metal anodes, characterized by issues such as dendrite growth and parasitic side reactions, poses a significant barrier to widespread applications. Herein, we address this challenge by designing a localized conjugated structure comprising a cyclic polyacrylonitrile polymer (CPANZ), induced by a Zn-based Lewis acid (zinc trifluoromethylsulfonate) at a temperature of 120 °C.

Integrated Bulk and Single-cell RNA Sequencing Data Constructs and Validates a Prognostic Model for Non-small Cell Lung Cancer.

: Most of the current research on prognostic model construction for non-small cell lung cancer (NSCLC) only involves in bulk RNA-seq data without integration of single-cell RNA-seq (scRNA-seq) data. Besides, most of the prognostic models are constructed by predictive genes, ignoring other predictive variables such as clinical features. : We obtained scRNA-seq data from GEO database and bulk RNA-seq data from TCGA database.

PMab-301: An Anti-Giraffe Podoplanin Monoclonal Antibody for Immunohistochemistry.

Immunohistochemistry staining is an essential method in pathological diagnoses. Podoplanin (PDPN) is a specific maker of alveolar epithelium, lymphatic vessels, and glomeruli. In this study, we established a novel anti-giraffe PDPN (girPDPN) mAb, PMab-301, using the Cell-Based Immunization and Screening (CBIS) method.

A biocompatible electrolyte enables highly reversible Zn anode for zinc ion battery.

Progress towards the integration of technology into living organisms requires power devices that are biocompatible and mechanically flexible. Aqueous zinc ion batteries that use hydrogel biomaterials as electrolytes have emerged as a potential solution that operates within biological constraints; however, most of these batteries feature inferior electrochemical properties. Here, we propose a biocompatible hydrogel electrolyte by utilising hyaluronic acid, which contains ample hydrophilic functional groups.

Integration of scRNA-seq and bulk RNA-seq constructs a stemness-related signature for predicting prognosis and immunotherapy responses in hepatocellular carcinoma.

Purpose: Cancer stem cells are associated with unfavorable prognosis in hepatocellular carcinoma (HCC). However, existing stemness-related biomarkers and prognostic models are limited.

Methods: The stemness-related signatures were derived from taking the union of the results obtained by performing WGCNA and CytoTRACE analysis at the bulk RNA-seq and scRNA-seq levels, respectively.

Soft-Rigid Heterostructures with Functional Cation Vacancies for Fast-Charging and High-Capacity Sodium Storage.

Optimizing charge transfer and alleviating volume expansion in electrode materials are critical to maximize electrochemical performance for energy-storage systems. Herein, an atomically thin soft-rigid Co S @MoS core-shell heterostructure with dual cation vacancies at the atomic interface is constructed as a promising anode for high-performance sodium-ion batteries. The dual cation vacancies involving V and V in the heterostructure and the soft MoS shell afford ionic pathways for rapid charge transfer, as well as the rigid Co S core acting as the dominant active component and resisting structural deformation during charge-discharge.