The S-nitrosylation of monodehydroascorbate reductase positively regulated the low temperature tolerance of mini Chinese cabbage.

One of the main environmental stresses that considerably reduced vegetable yields are low temperature stress. Brassinosteroids (BRs) is essential for controlling a number of physiological functions. Protein S-nitrosylation is thought to be a crucial process in plants that use NO to carry out their biological functions.

Comprehensive transcriptomic and metabolomic analysis of porcine intestinal epithelial cells after PDCoV infection.

Introduction: Porcine deltacoronavirus (PDCoV), an emerging swine enteropathogenic coronavirus with worldwide distribution, mainly infects newborn piglets with severe diarrhea, vomiting, dehydration, and even death, causing huge economic losses to the pig industry. However, the underlying pathogenic mechanisms of PDCoV infection and the effects of PDCoV infection on host transcripts and metabolites remain incompletely understood.

Methods: This study investigated a combined transcriptomic and metabolomic analysis of porcine intestinal epithelial cells (IPEC-J2) following PDCoV infection by LC/MS and RNA-seq techniques.

Melatonin Modulates Tomato Root Morphology by Regulating Key Genes and Endogenous Hormones.

Melatonin plays a vital role in plant growth and development. In this study, we treated hydroponically grown tomato roots with various concentrations of exogenous melatonin (0, 10, 30, and 50 μmol·L). We utilized root scanning and microscopy to examine alterations in root morphology and cell differentiation and elucidated the mechanism by which melatonin regulates these changes through the interplay with endogenous hormones and relevant genes.

Integrated metabolomics and transcriptomics analyses reveal metabolic responses to TGEV infection in porcine intestinal epithelial cells.

Transmissible gastroenteritis virus (TGEV) is a coronavirus that infects piglets with severe diarrhoea, vomiting, dehydration, and even death, causing huge economic losses to the pig industry. The underlying pathogenesis of TGEV infection and the effects of TGEV infection on host metabolites remain poorly understood. To investigate the critical metabolites and regulatory factors during TGEV infection in intestinal porcine epithelial cells (IPEC-J2), we performed metabolomic and transcriptomic analyses of TGEV-infected IPEC-J2 cells by LC/MS and RNA-seq techniques.

Lateral Habenula Neurons Signal Cold Aversion and Participate in Cold Aversion.

The aversion to cold is a fundamental motivated behavior that contributes to the body temperature homeostasis. However, the involvement of the lateral habenula (LHb) as a regulatory hub for negative emotions in this physiological process remains uninvestigated. In this study, we demonstrate an elevation in the population activity of LHb neurons following exposure to cold stimuli.

Integrated of multi-omics and molecular docking reveal PHGDH, PSAT1 and PSPH in the serine synthetic pathway as potential targets of T-2 toxin exposure in pig intestinal tract.

T-2 toxin (T-2) with a molecular weight of 466.52 g/mol is an inevitable mycotoxin in food products and feeds, posing a significant threat to human and animal health. However, the underlying molecular mechanisms of the cytotoxic effects of T-2 exposure on porcine intestinal epithelial cells (IPEC-J2) remain unclear.

Aqueous M. oleifera leaf extract alleviates DSS-induced colitis in mice through suppression of inflammation.

Ethnopharmacological Relevance: Moringa oleifera Lam. (M. oleifera) is a perennial deciduous tree with considerable agricultural and pharmacological value.

Porcine Deltacoronavirus Infection Disrupts the Intestinal Mucosal Barrier and Inhibits Intestinal Stem Cell Differentiation to Goblet Cells via the Notch Signaling Pathway.

Goblet cells and their secreted mucus are important elements of the intestinal mucosal barrier, which allows host cells to resist invasion by intestinal pathogens. Porcine deltacoronavirus (PDCoV) is an emerging swine enteric virus that causes severe diarrhea in pigs and causes large economic losses to pork producers worldwide. To date, the molecular mechanisms by which PDCoV regulates the function and differentiation of goblet cells and disrupts the intestinal mucosal barrier remain to be determined.

Study on Multi-Mode Switching Control Strategy of Active Suspension Based on Road Estimation.

In this paper, the least squares method is used to determine the vertical height of the road space domain. Based on the road estimation method, the active suspension control mode switching model is constructed, and the dynamic characteristics of the vehicle in comfort, safety, and integrated modes are analyzed. The vibration signal is collected by the sensor, and the parameters such as vehicle driving conditions are solved for in reverse.

Synthesis of AgNPs from waste mobile phone circuit boards using an emulsion liquid membrane method.

The disposal of mobile phone print circuit boards (WMPCBs) has now been a problem because of the continually increasing production of mobile phones. It could be considered a new source of precious metal but may jeopardize the environment and human health without proper management. An emulsion liquid membrane (ELM) method is applied and improved to recover silver from WMPCBs and prepare AgNPs.

Application of Exogenous Melatonin Improves Tomato Fruit Quality by Promoting the Accumulation of Primary and Secondary Metabolites.

Melatonin plays key roles in improving fruit quality and yield by regulating various aspects of plant growth. However, the effects of how melatonin regulates primary and secondary metabolites during fruit growth and development are poorly understood. In this study, the surfaces of tomato fruit were sprayed with different concentrations of melatonin (0, 50, and 100 µmol·L) on the 20th day after anthesis; we used high-performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS) to determine the changes in primary and secondary metabolite contents during fruit development and measured the activity of sucrose metabolizing enzymes during fruit development.

Impact of Wheat Arabinoxylan with Defined Substitution Patterns on the Heat-Induced Polymerization Behavior of Gluten.

To further depict the interaction mechanism of wheat arabinoxylan (AX) and gluten proteins upon thermal processing, AX was enzymatically tailored with defined substitution patterns and the impact on the heat-induced polymerization behavior of gluten was comparatively studied. The results showed that tailormade AX promoted the formation of glutenin-glutenin and glutenin-gliadin macrocrosslinks upon heating, with the optimal effect detected for AX depleted of Ara of disubstituted Xyl. The tailormade AX, especially AX depleted of monosubstituted Xyl, facilitated the polymerization ability of α-gliadin into glutenin compared with untailored AX.

Enhancing the technofunctionality of γ-aminobutyric acid enriched germinated wheat by modification of arabinoxylan, gluten proteins and liquid lamella of dough.

To enhance the technofunctionality of germinated wheat enriched with γ-aminobutyric acid, xylanase (Xyn) and glucose oxidase (Gox) were incorporated with emphasis on modifying the key components. Combination of Xyn and Gox enhanced steamed bread quality with optimum loaf volume and textural property. Continuous and dense gluten network was facilitated and improved viscoelasticity of dough.

Physicochemical, Nutritional Properties and Metabolomics Analysis Fat Deposition Mechanism of Chahua Chicken No. 2 and Yao Chicken.

Poultry is an important dietary source of animal protein, accounting for approximately 30% of global meat consumption. Because of its low price, low fat and cholesterol content, and no religious restrictions, chicken is considered a widely available healthy meat. Chahua chicken No.

The impact of subsidy and preferential tax policies on mobile phone recycling: A system dynamics model analysis.

This paper analyzes the impact of possible subsidy and preferential tax policies on mobile phone (MP) recycling industries in China using system dynamics methodology, aiming to offer a reference for the construction of a reasonable policy system for MP recycling. First, different subsidy and preferential tax policies were introduced to establish a system dynamics model. Then the impact of policies proposed on MP recycling industries was analyzed using the model.

Association between H19 polymorphisms and NSCLC risk in a Chinese Population.

Purpose: Long non-coding RNAs (lncRNA) polymorphisms have been reported to associate with the carcinogenicity mechanisms. The association between lncRNA H19 polymorphisms and the risk of non-small cell lung cancer (NSCLC) in Chinese population has not been reported yet. We designed this case-control study to evaluate the effects of H19 polymorphisms on NSCLC susceptibility.