High-sensitivity detection of two H7 subtypes of avian influenza viruses (AIVs) by immunochromatographic assay with highly chromatic red silica nanoparticles.

In this work, a sensitive and quantitative immunochromatographic assay (ICA) detection method for avian influenza viruses (AIVs) of the H7 hemagglutinin (HA) antigen was established based on highly chromatic red silica nanoparticles (SiNPs). It can detect two H7 subtypes of influenza viruses, H7N2 and H7N9. The highly chromatic red SiNPs were prepared by adsorbing C.

High expression of vinculin predicts poor prognosis and distant metastasis and associates with influencing tumor-associated NK cell infiltration and epithelial-mesenchymal transition in gastric cancer.

In the process of epithelial-mesenchymal transition (EMT), epithelial cancer cells transdifferentiate into mesenchymal-like cells with high motility and aggressiveness, resulting in the spread of tumor cells. Immune cells and inflammation in the tumor microenvironment are the driving factors of EMT, but few studies have explored the core targets of the interaction between EMT and tumor immune cells. We analyzed thousands of cases of gastric cancer and gastric tissue specimens of TCGA, CPTAC, GTEx and analyzing QPCR and IHC data of 56 gastric cancer patients in SYSU Gastric Cancer Research Center.

Determination of trace aflatoxin M1 (AFM1) residue in milk by an immunochromatographic assay based on (PEI/PSS) red silica nanoparticles.

Aflatoxin M1 (AFM1) residues in milk pose a major threat to human health, so there is an urgent need for a simple, rapid, and sensitive method for the determination of trace AFM1 in milk. In this study, a competitive immunochromatographic assay (ICA), using visual (PEI/PSS) red silica nanoparticles (SiNPs) as signal amplification probes, was used for the highly sensitive detection of AFM1. The (PEI/PSS) red SiNPs were used to label AFM1 monoclonal antibody (mAb) to prepare ICA for the detection of AFM1.

Ultramarine blue nanoparticles as a label for immunochromatographic on-site determination of ractopamine.

A competitive immunochromatographic assay (ICA) is presented and used for on-site determination of ractopamine (RAC). Ultramarine blue nanoparticles were directly separated from ultramarine blue industrial products by centrifugation (< 10,000 rpm and > 4000 rpm) and used as visible labels in ICAs. The ultramarine blue nanoparticles were coated by polyacrylic acid (PAA), which provides carboxyl groups on the surface of ultramarine blue nanoparticles.

A novel immunochromatographic assay using ultramarine blue particles as visible label for quantitative detection of hepatitis B virus surface antigen.

Ultramarine blue particles as a novel visible label has been used to develop immunochromatographic assay (ICA). The ultramarine blue particles, as a sodalite mineral with formula: (Na,Ca)[(S,Cl,SO,OH)(AlSiO)], can generate a blue visible signal were used as a label for ICA. Ultramarine blue particles were applied to a sandwich immunoassay to detect hepatitis B virus surface antigen (HBsAg).

A silica nanoparticle based 2-color immunochromatographic assay for simultaneous determination of clenbuterol and ractopamine.

An immunochromatographic assay (ICA) is presented that can be applied to simultaneous detection of clenbuterol (CLE) and ractopamine (RAC). It is making use of two red and blue silica nanoparticles (SiNPs) that act as labels for encoding the antibodies. This design permits multiplexed analysis in a single test line and does not require an external source for photoexcitation.

Core-shell red silica nanoparticles based immunochromatographic assay for detection of Escherichia coli O157:H7.

In this paper, a new type immunochromatographic assay (ICA) based on core-shell red silica nanoparticles (core-shell red SiONPs) was proposed and used to detect Escherichia coli O157:H7 (E. coli O157:H7). This is the first report of qualitative ICA for detecting E.

Electrochemical sandwich immunoassay for Escherichia coli O157:H7 based on the use of magnetic nanoparticles and graphene functionalized with electrocatalytically active Au@Pt core/shell nanoparticles.

A highly sensitive electrochemical sandwich immunoassay is described for determination of Escherichia coli O157:H7 (E. coli O157:H7). Silica coated magnetite nanoparticles (FeO) were modified with primary antibody to capture E.

A non-enzymatic electrochemical immunoassay for quantitative detection of Escherichia coli O157:H7 using Au@Pt and graphene.

Herein, a non-enzymatic sandwich-type electrochemical immunoassay was fabricated for quantitative monitoring of Escherichia coli O157:H7 (E. coli O157:H7). Silica coated FeO magnetic nanoparticles (FeO@SiO) were modified with mouse anti-E.

Voltammetric sandwich immunoassay for Cronobacter sakazakii using a screen-printed carbon electrode modified with horseradish peroxidase, reduced graphene oxide, thionine and gold nanoparticles.

The authors describe a sandwich-type of electrochemical immunoassay for rapid determination of the foodborne pathogen Cronobacter sakazakii (C. sakazakii). Polyclonal antibody against C.

An electrochemical immunoassay for Escherichia coli O157:H7 using double functionalized Au@Pt/SiO nanocomposites and immune magnetic nanoparticles.

A sensitive Point-of-Care Testing (POCT) with Au-Pt bimetallic nanoparticles (Au@Pt) functionalized silica nanoparticle (SiO NPs) and FeO magnetic nanoparticles (FeO NPs) was designed for the quantitative detection of Escherichia coli O157:H7 (E. coli O157:H7). The poly-(4-styrenesulfonic acid-co-maleic acid) (PSSMA) as a negatively charged polyelectrolyte can be easily coated on surface of the amino group modified SiO NPs via electrostatic force.

Portable and quantitative point-of-care monitoring of Escherichia coli O157:H7 using a personal glucose meter based on immunochromatographic assay.

Here we innovate a portable and quantitative immunochromatographic assay (ICA) with a personal glucose meter (PGM) as readout for the detection of Escherichia coli O157:H7 (E. coli O157:H7). The carboxyl group coated FeO nanoparticles (MNPs) were synthesized by a one pot method and used as carriers of invertase and monoclonal antibody against E.

Rapid electrochemical quantification of Salmonella Pullorum and Salmonella Gallinarum based on glucose oxidase and antibody-modified silica nanoparticles.

In this article, a facile and sensitive electrochemical method for quantification of Salmonella Pullorum and Salmonella Gallinarum (S. Pullorum and S. Gallinarum) was established by monitoring glucose consumption with a personal glucose meter (PGM).

A nonenzymatic optical immunoassay strategy for detection of Salmonella infection based on blue silica nanoparticles.

A novel nonenzymatic optical immunoassay strategy was for the first time designed and utilized for sensitive detection of antibody to Salmonella pullorum and Salmonella gallinarum (S. pullorum and S. gallinarum) in serum.

Simultaneous detection of pathogenic bacteria using agglutination test based on colored silica nanoparticles.

Aimed to explore an agglutination test which can simultaneously detect two pathogenic bacteria, an agglutination test based on colored silica nanoparticles (colored-SiNps) was established in this work. Monodisperse colored-SiNps were used as agglutination test carriers; red-SiNps and blue-SiNps were prepared by reverse microemulsion with C.I.

Immunosensor based on electrodeposition of gold-nanoparticles and ionic liquid composite for detection of Salmonella pullorum.

In order to increase the reproducibility and stability of electrochemical immunosensor, which is a key issue for its application and popularization, an accurate and stable immunosensor for rapid detection of Salmonella pullorum (S. pullorum) was proposed in this study. The immunosensor was fabricated by modifying Screen-printed Carbon Electrode (SPCE) with electrodeposited gold nanoparticles (AuNPs), HRP-labeled anti-S.

Performance of the subsurface flow constructed wetlands for pretreatment of slightly polluted source water.

The slightly polluted source water of Yellow River was pretreated in a horizontal subsurface flow constructed wetland (HSFCW) and a lateral subsurface flow constructed wetland (LSFCW) in the Ji'nan city Reservoir, Shandong, China. During almost one years run, the results showed that at the hydraulic loading rate of 1 m/day, the removal efficiencies of chemical oxygen demand (COD), total nitrogen (TN), ammonium nitrogen (NH4 (+)-N) and total phosphorus (TP) in the HSFCW were 48.9, 51.

A disposable immunosensor for Enterobacter sakazakii based on an electrochemically reduced graphene oxide-modified electrode.

A disposable immunosensor based on electrochemically reduced graphene oxide (ERGO) was developed for the detection of Enterobacter sakazakii. First, the graphene was deposited on a screen-printed carbon electrode (SPCE) by an electrochemical method. Second, the horseradish peroxidase-labeled bacteria-specific antibody was assembled onto the modified electrode to enhance the sensitivity of the immunosensor.

Graphene-coated fiber for solid-phase microextraction of triazine herbicides in water samples.

Graphene is a novel and interesting carbon material that could be used for the separation and purification of some chemical compounds. In this investigation, graphene was used as a novel fiber-coating material for the solid-phase microextraction (SPME) of four triazine herbicides (atrazine, prometon, ametryn and prometryn) in water samples. The main parameters that affect the extraction and desorption efficiencies, such as the extraction time, stirring rate, salt addition, desorption solvent and desorption time, were investigated and optimized.

Determination of triazine herbicides in environmental water samples by high-performance liquid chromatography using graphene-coated magnetic nanoparticles as adsorbent.

In this paper, a graphene-based Fe(3)O(4) magnetic nanoparticles (G-Fe(3)O(4) MNPs) was used as the adsorbent for the magnetic solid-phase extraction of some triazine herbicides (atrazine, prometon, propazine and prometryn) in environmental water samples followed by high performance liquid chromatography-diode array detection (HPLC-DAD). After the extraction, the adsorbent can be conveniently separated from the aqueous samples by an external magnet. The main factors influencing the extraction efficiency including the amount of the MNPs, the extraction time, the pH of sample solution, and desorption conditions were studied and optimized.

Preparation of a graphene-based magnetic nanocomposite for the extraction of carbamate pesticides from environmental water samples.

A graphene-based magnetic nanocomposite was synthesized and used for the first time as an effective adsorbent for the preconcentration of the five carbamate pesticides (metolcarb, carbofuran, pirimicarb, isoprocarb and diethofencarb) in environmental water samples prior to high performance liquid chromatography-diode array detection. The properties of the magnetic nanocomposite were characterized by scanning electron microscopy and X-ray diffraction. This novel graphene-based magnetic nanocomposite showed great adsorptive ability towards the analytes.

[Effects of elevated atmospheric CO2 concentration and nitrogen addition on the growth of Calamagrostis angustifolia in Sanjiang Plain freshwater marsh].

By using open top chamber, an experiment with two levels of atmospheric CO2 concentration (350 and 700 micromol x mol(-1)) and three levels of nitrogen supply (0, 5, and 15 g N x m(-2)) was conducted to investigate the effects of elevated atmospheric CO2 and nitrogen supply on the growth of Calamagrostis angustifolia in the freshwater marsh of Sanjiang Plain. Under elevated atmospheric CO2 concentration, the phenophase of C. angustifolia advanced.

[Development of the soft independent modelling of class analogies model to discrimination Vibrio parahemolyticus by Smartongue].

Objective: To explore a new rapid detection method for detecting of Food pathogens.

Method: We used the Smartongue, to determine the composition informations of the liquid culture samples and combined with soft independent modelling of class analogies (SIMCA) to analyze their respective species, then set up a Smartongue -SIMCA model to discriminate the V. parahaemolyticus.