Exosomal AHSG in ovarian cancer ascites inhibits malignant progression of ovarian cancer by p53/FAK/Src signaling.

Background: The primary cause of mortality in patients with ovarian cancer (OC) is tumor metastasis. A comprehensive understanding of the mechanisms underlying metastasis in OC is essential for accurate prognosis prediction and the development of targeted therapeutic agents. Our findings indicate that alpha-2 Heremans Schmid glycoprotein (AHSG) is downregulated in OC exosomes.

Jujuboside A Attenuates Polycystic Ovary Syndrome Based on Estrogen Metabolism Through Activating AhR-mediated CYP1A2 Expression.

Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders in women. This study aimed to investigate the therapeutic effects and mechanism of Jujuboside A on PCOS using a dehydroepiandrosterone (DHEA)-induced PCOS mouse model. Estrogen and androgen homeostasis was evaluated in serum from both clinical samples and PCOS mice.

BiVO prepared by the sol-gel doped on graphite felt cathode for ciprofloxacin degradation and mechanism in solar-photo-electro-Fenton.

In this research, bismuth vanadate-doped graphite felt (GF-BiVO) was successfully prepared by sol-gel method, in which BiVO owned superior electro-Fenton (EF) and solar-photo-electro-Fenton (SPEF) performance. Combined with the analysis by X-ray diffractometer (XRD), field emission transmission electron microscopy (FE-TEM), nitrogen adsorption-desorption isotherms and cyclic voltammetry (CV), the changes of electrodes were reflected in structure and physicochemical properties. The doping of monoclinic BiVO endued GF with a higher surface area and more electro-active sites and better electrode activity in comparison to Raw-GF.

Anodic oxidation of ciprofloxacin using different graphite felt anodes: Kinetics and degradation pathways.

Ciprofloxacin (CIP) is ubiquitous in the environment which poses a certain threat to human and ecology. In this investigation, the physical and electrochemical properties of graphite felt (GF) anodes which affected the anodic oxidation (AO) performance, and the CIP removal effect of GF were evaluated. The GFs were used as anodes for detection of ·OH with coumarin (COU) as molecule probe and removal of CIP in a 150 mL electrolytic cell with Pt cathode (AO-GF/Pt system).

Preparation of CeO-doped carbon nanotubes cathode and its mechanism for advanced treatment of pig farm wastewater.

The effluent from conventional treatment process (including anaerobic digestion and anoxic-oxic treatment) for pig farm wastewater was difficult to treat due to its low ratio of biochemical oxygen demand to chemical oxygen demand (BOD/COD) (<0.1). In the present study, electro-Fenton (EF) was used to improve the biodegradability of the mentioned effluent and the properties of self-prepared CeO-doped multi-wall carbon nanotubes (MWCNTs) electrodes were also studied.

Accumulation and toxicity of multi-walled carbon nanotubes in Xenopus tropicalis tadpoles.

Multi-walled carbon nanotubes (MWCNTs), a common nanomaterial widely used and discharged in environment, might exert toxic effects on aquatic animals. In this paper, filter-feeding tadpole of Xenopus tropicalis was selected as bioindicator to study the exposure effects of MWCNTs suspensions of 0.5, 1, 2, 4 and 8 mg/L for 72 h.

Kinetics and mechanisms of oxytetracycline degradation in an electro-Fenton system with a modified graphite felt cathode.

The removal of trace antibiotics from the aquatic environment has received great interest. In this investigation, NaOH activated graphite felt (NaOH-GF) was characterized by multiple-methods, including scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), contact angle, linear sweep voltammetry (LSV) and electron paramagnetic resonance (EPR). The NaOH-GF was then used as the cathode in the electro-Fenton process for oxytetracycline (OTC) degradation, the experiment was carried out in an undivided and light-proof beaker with a Pt anode and a NaOH-GF cathode at pH 3.

Effective removal of nitrate by denitrification re-enforced with a two-stage anoxic/oxic (A/O) process from a digested piggery wastewater with a low C/N ratio.

The combined process of a long-term biogas digester and double anoxic/oxic tanks is very commonly used in piggery wastewater treatment in South China, but the effluent does not meet the discharge standard of total nitrogen (TN) and chemical oxygen demand (COD) due to a low C/N ratio and insufficient organic carbon in digested piggery wastewater. Thus, a typical two-stage anoxic/oxic (A1/O1/A2/O2) process, which is widely used to treat digested piggery wastewater in the engineering application, was selected for study on a laboratory scale. Finally, the average removal efficiency of ammonia nitrogen in the two-stage AO process was 98.

Evolution of corresponding resistance genes in the water of fish tanks with multiple stresses of antibiotics and heavy metals.

Abuse of antibiotics and heavy metals in aquaculture has been widely concerned and might aggravate the spread of resistance genes in environment. To investigate the occurrence and proliferation of antibiotic resistance genes (ARGs) and heavy metal resistance genes (HMRGs), three commonly used antibiotics (tetracycline, sulfanilamide, cefotaxime) and two heavy metals (Zn and Cu) were designed to add individually or jointly in nine fish tanks including five individual exposure tanks of tetracycline (tet), sulfanilamide (sul), cefotaxime (cef), Cu, Zn and four combination exposure tanks of tetracycline + sulfanilamide (tet + sul), tetracycline + sulfanilamide + cefotaxime (tet + sul + cef), tetracycline + sulfanilamide + Cu (tet + sul + Cu), tetracycline + sulfanilamide + Zn (tet + sul + Zn) as well as the control during the experiment period of 180 days. Nineteen ARGs (tetA, tetB, tetC, tetD, tetE, tetG, tetM, tetO, tetQ, tetS, tetW, tetX, tetY, sul1, sul2, sul3, bla, bla, bla), two HMRGs (copA, czcA) and the class 1 integron gene (intI 1) in fish tanks water were investigated.

Genome-wide association study on susceptibility genes associated with yang-deficiency constitution: A small sample case-control study.

Objective: To explore susceptibility genes associated with yang-deficiency constitution using single nucleotide polymorphism (SNP) genotyping.

Methods: Based on an epidemiological survey, 30 volunteers with yang-deficiency constitution and 30 volunteers with a balanced constitution were included according to the Classification and Determination Standards of Constitutions in Traditional Chinese Medicine. Peripheral blood was collected and DNA was extracted from white blood cells.

Prediction and identification of potential immunodominant epitopes in glycoproteins B, C, E, G, and I of herpes simplex virus type 2.

Twenty B candidate epitopes of glycoproteins B (gB2), C (gC2), E (gE2), G (gG2), and I (gI2) of herpes simplex virus type 2 (HSV-2) were predicted using DNAstar, Biosun, and Antheprot methods combined with the polynomial method. Subsequently, the biological functions of the peptides were tested via experiments in vitro. Among the 20 epitope peptides, 17 could react with the antisera to the corresponding parent proteins in the EIA tests.

[Analysis of composite traditional Chinese medicine constitution: an investigation of 974 volunteers].

Objective: To explore the analysis methods for composite traditional Chinese medicine (TCM) constitutions.

Methods: The epidemiological data of TCM constitutions were collected from 974 volunteers via a cross-sectional survey. The samples were classified into 9 constitution types according to the maximal standardized scores of imbalanced constitutions.

Design and evaluation of a multi-epitope assembly peptide (MEAP) against herpes simplex virus type 2 infection in BALB/c mice.

Background: Human herpes simplex virus (HSV) 1 and 2 causes oral, ocular, or genital infections, which remains a significant health problem worldwide. HSV-1 and -2 infections in humans range from localized skin infections of the oral, ocular, and genital regions to severe and often disseminated infections in immunocompromised hosts. Epitope based vaccination is a promising mean to achieve protective immunity and to avoid infections with Human herpes simplex virus type 2 (HSV-2).

Establishment of a novel model for studying the effects of extracts of Chinese herb medicine on human type II 5alpha-reductase in vitro.

Human steroid 5alpha-reductase type II (hSRD5A2) and dihydrotestosterone (DHT) play important roles in benign prostatic hyperplasia (BPH). The aim of our study was to establish a novel model to investigate the inhibitory effects of extracts and compounds of Chinese herb medicine on hSRD5A2. The gene, hSRD5A2, was artificially synthesized and cloned into pcDNA3.