Optimizing Biomarker Models for Biologically Heterogeneous Cancers: A Nested Model Approach for Lung Cancer.

Background: The heterogeneous biology of cancer subtypes, especially in lung cancer, poses significant challenges for biomarker development. Standard model building techniques often fall short in accurately incorporating various histologic subtypes because of their diverse biological characteristics. This study explores a nested biomarker model to address this issue, aiming to improve lung cancer early detection.

Continuous R-DA-EDOCH alternated with high-dose Ara-C induces deep remission and overcomes high-risk factors in young patients with newly diagnosed mantle cell lymphoma.

Objective: Our previous studies have indicated potentially higher proliferative activity of tumor cells in Chinese patients with mantle-cell lymphoma (MCL) than those in Western. Given the success and tolerability of R-DA-EDOCH immunochemotherapy in treating aggressive B-cell lymphomas, we designed a prospective, phase 3 trial to explore the efficacy and safety of alternating R-DA-EDOCH/R-DHAP induction therapy for young patients with newly diagnosed MCL. The primary endpoint was the complete remission rate (CRR) at the end of induction (EOI).

Workplace Violence Against Primary Care Physicians in Chengdu, China: A Cross-sectional Survey.

Background: The purpose of this study is to examine workplace violence (WPV) toward primary care physicians (PCPs), including prevalence, associated factors, impacts, and response to WPV in Chengdu, China.

Methods: We used an online cross-sectional design to collect data from October to November 2022 with a structured self-administered questionnaire from a purposive sample of 568 PCPs in Chengdu city.

Results: Among the 490 valid questionnaires, 44.

Multidependency Graph Convolutional Networks and Contrastive Learning for Drug Repositioning.

The goal of drug repositioning is to expedite the drug development process by finding novel therapeutic applications for approved drugs. Using multifeature learning, different computational drug repositioning techniques have recently been introduced to predict possible drug-disease relationships. Nevertheless, current graph-based methods tend to model drug-disease interaction relationships without considering the semantic influence of node-specific side information on graphs.

Exploring the impact of dietary factors on intracranial aneurysm risk: insights from Mendelian randomization analysis.

Background: While existing research has established a link between dietary habits and the incidence of intracranial aneurysms, the application of Mendelian randomization to explore this association remains largely uncharted.

Methods: In our study, we analyzed a wide array of dietary factors using data from the IEU Open GWAS project, which included meat varieties, vegetarian foods, cereal and the frequency of alcohol intake. We included pooled intracranial aneurysm GWAS data from a comprehensive dataset of 7,495 cases.

POGZ targeted by LINC01355/miR-27b-3p retards thyroid cancer progression via interplaying with MAD2L2.

Unlabelled: Despite the high morbidity of thyroid cancer (THCA), the underlying molecular pathology remains elusive. That autism-associated protein POGZ has recently been involved in tumorigenesis intrigues us exploring its relevant molecular regulatory network in THCA. Clinical characteristics and intermolecular relationships were dissected by bioinformatics.

Global trends in gallbladder cancer survival: A 30-year analysis of cancer registry data.

Background: Gallbladder cancer has historically been characterized with a poor prognosis. This study aims to describe the global patterns and temporal trends in gallbladder cancer survival using data from cancer registries.

Methods: We conducted a systematic review by searching six databases-PubMed, Web of Science, EMBASE, SEER, CNKI, and Wanfang-for using of registry-based data published before January 1, 2024.

The correlation of starch composition, physicochemical and structural properties of different sorghum grains.

The composition, structure, and physicochemical properties of starch in sorghum grains greatly influence the processing and quality of the final products. In this study, 19 sorghum lines were examined to analyze various starch-related characteristics. Correlation analysis of these key traits, revealed a significant correlation between amylose and amylopectin content.

Jianpi Yangzheng Xiaozheng granule induced ferroptosis to suppress gastric cancer progression through reprogramming lipid metabolism via SCD1/Wnt/β-catenin axis.

The incidence of Poorly cohesive carcinoma (PCC) has steadily risen in recent years, posing a significant clinical challenge. To reveal the anti-tumor effects of Jianpi Yangzheng Xiaozheng granule (JPYZXZ) in PCC, an initial investigation was performed using CCK-8, colony formation, scratch, and transwell assays. This was followed by network pharmacology studies to gain a deeper understanding of JPYZXZ's impact on gastric cancer (GC).

Time-varying cost-effectiveness analysis of sodium-glucose cotransporter-2 inhibitors in Chinese patients with heart failure and reduced ejection fraction: A microsimulation of the real-world population.

Objective: Sodium-glucose cotransporter-2 (SGLT2) inhibitors showed time-varying effects in heart failure and reduced ejection fraction (HFrEF), but corresponding cost-effectiveness in different timeframes remained poorly understood. This study estimated the time-varying cost-effectiveness of SGLT2 inhibitors in HFrEF from the perspective of the Chinese healthcare system.

Methods: Based on real-world individual patient data, a 2-year microsimulation model was constructed to evaluate the cost-effectiveness of adding SGLT2 inhibitors to standard therapy compared with standard therapy alone among patients with HFrEF.

ATP1A3 Acts as a Potential Anti-oncogene in Glioblastoma via the Antagonizing Interaction with Small Nuclear Ribonucleoprotein Polypeptide G.

Background: The sodium pump α3 subunit (ATP1A3) is associated with various brain's physiological and pathological mechanisms. However, its molecular mechanisms and cellular targets in glioblastoma (GBM) are poorly understood.

Methods: Bioinformatics and phosphor-proteomics analysis, target fishing experiment, confocal immunofluorescence, molecular cloning, and western blot techniques were carried out to elucidate probable downstream signaling pathways.

Microplastic-Induced Alterations in Soil Aggregate-Associated Carbon Stabilization Pathways: Evidence from δC Signature Analysis.

Microplastics (MPs) are known to affect soil carbon stability in a numerous ways. However, the mechanisms by which they alter the carbon stability within soil aggregates remain unclear . Herein, a one-year field experiment was conducted in an arid agricultural region employing stable isotope techniques to evaluate the soil organic carbon flow in the presence of both persistent (PE, PVC) and biodegradable (PLA, PHA) MPs.

Biosynthesis of the Central Tricyclic Skeleton of Trichothecene Mycotoxins.

Trichothecenes are a widespread family of sesquiterpenoid toxins that can pose significant risks to food and feed safety as well as environmental health. A defining feature of all trichothecenes is their central tricyclic 12,13-epoxytrichothec-9-ene (EPT) motif. Although the formation of the EPT central skeleton has long been presumed to be a spontaneous process, the nonenzymatic cyclization reaction forming the tetrahydropyran ring in EPT requires acid catalysis; otherwise, it occurs too slowly to sustain efficient trichothecene biosynthesis under physiological conditions.

Young fibroblast-derived migrasomes alleviate keratinocyte senescence and enhance wound healing in aged skin.

Background: Alterations in intercellular communication driven by cellular senescence constitute an important factor in skin aging. Migrasome, a newly discovered vesicular organelle, efficiently participates in intercellular communication; however, the relationship between cellular senescence and migrasomes remains unreported.

Objective: This study aims to explore the possible relationship between cellular senescence and migrasomes formation, and investigate the effects of young fibroblast-derived migrasomes on senescent keratinocytes and wound healing in aged skin.

AnomalGRN: deciphering single-cell gene regulation network with graph anomaly detection.

Background: Single-cell RNA sequencing (scRNA-seq) is now essential for cellular-level gene expression studies and deciphering complex gene regulatory mechanisms. Deep learning methods, when combined with scRNA-seq technology, transform gene regulation research into graph link prediction tasks. However, these methods struggle to mitigate the impact of noisy data in gene regulatory networks (GRNs) and address the significant imbalance between positive and negative links.

Single-cell analysis identifies MKI67 microglia as drivers of neovascularization in proliferative diabetic retinopathy.

Background: Proliferative diabetic retinopathy (PDR) is among the primary causes of blindness in individuals with diabetes. Elevated lactate levels have been identified as a critical biomarker associated with the prognosis of PDR. While significant lactate accumulation has been observed in the vitreous fluid of PDR patients, the detailed pathways through which lactate impacts pathological neovascularization remain insufficiently elucidated.

Altered KEGG pathways are associated with different immunologic responses to antiretroviral therapy in HIV-infected men who have sex with men.

Background: The association between Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolic pathways and immunologic non-response among people living with HIV (PLHIV) on antiretroviral therapy (ART) is not well documented. This study aimed to characterize KEGG metabolic pathways among HIV-infected men who have sex with men (MSM) with different immunologic responses.

Methods: We recruited HIV-uninfected MSM (healthy controls, HC) and HIV-infected MSM on ART > 24 months in Guangzhou, June-October 2021.

Effects of different concentrations of chlormequat chloride on bacterial community composition and diversity in peanut soil.

The application of pesticides may have significant impacts on soil environment and communities. In order to understand the deep relationship between the application of chlormequat chloride (CC) and the bacterial community in peanut soil, high-resolution characterization was performed using peanut soil samples (12 points; 0-20 cm rhizosphere soil) from untreated and sprayed with different concentrations of CC. Experimental data showed that with the increase of concentration, operational taxonomic units (OTUs) richness showed a decreasing tendency.

Mediation by elevated prolactin in the relationship between childhood trauma and first-episode drug-naïve schizophrenia.

Background: The elevated prolactin levels in first-episode drug-naïve (FEDN) schizophrenia patients may correlate with long-term stress caused by childhood trauma. This study aimed to assess the relationship between elevated prolactin levels and childhood trauma in FEDN schizophrenia patients, while also considering sex differences.

Methods: Utilizing a cross-sectional design, the study involved 88 FEDN schizophrenia patients and 76 healthy controls (HCs).

A novel index evaluating left atrioventricular coupling function in chronic kidney disease with diabetes patients.

The left atrioventricular coupling index (LACI) evaluates the coupling function between the left atrial (LA) and left ventricular (LV) chambers. However, little is known about LACI in chronic kidney disease (CKD) patients. This study aimed to assess the impact of concurrent CKD and type 2 diabetes mellitus (T2DM) on left atrioventricular coupling function compared to CKD patients without T2DM.

Vaginal microbiota transplantation alleviates vaginal atrophy in ovariectomized mice.

Vaginal atrophy is a prevalent symptom in menopausal women, affecting over 50% of older women and patients with loss of ovarian function. The role of factors other than estrogen, such as the vaginal microbiota (VM), in the development of vaginal atrophy has not been fully explored. Therefore, we selected 8-week-old C57 mice with bilateral ovariectomy for experimentation.

Sub-minute synthesis and modulation of β/λ-MTiO ceramics towards accessible heat storage.

Nearly 50% of global primary energy consumption is lost as low-temperature heat. λ-TiO holds promise for waste heat harvesting and reuse; however, achieving reversible phase transitions between its λ and β phases under accessible conditions remains a major challenge. Here, we proposed a simple laser method that incorporates element substitution for sub-minute synthesis (20-60 s) of λ-MTiO (M = Mg, Al, Sc, V, Cr, Mn, or Fe, 0.

Thalidomide-based regimen shows promising efficacy in large granular lymphocytic leukemia: a multicenter phase II study.

Large granular lymphocytic leukemia (LGLL) is characterized by the clonal proliferation of cytotoxic T lymphocytes or NK cells. Standard first-line immunosuppressive treatments have limitations, achieving complete remission (CR) rates of up to 50%. Immune system dysregulation is implicated in LGLL.

Discovery of tri(indolyl)methanes as potent and selective inhibitors against human carboxylesterase 2.

Human carboxylesterase 2A (CES2A) plays a crucial role in the hydrolysis and metabolic activation of esters and amides. There is increasing evidence that development of CES2A inhibitors to modulate the hydrolysis of ester drugs will increase the potency of these drugs or reduce their side effects. In this study, three sets of indole analogues (4a-j, 5a-k, 6a-e) were designed and synthesized.

Unique isophorone-based NIR fluorescent probe with a high fluorescence quantum yield and larger stokes shift for Al and Zn level simultaneous monitoring in biological system.

Given the profound implications of Al and Zn in the ecosystem and organism, there is a compelling demand for a robust method to simultaneously detect these ions. The novel near-infrared (NIR) fluorescent probe DDLH based on isophorone, offers efficient in vivo monitoring capabilities for Al/Zn. Simultaneously, DDLH displayed excellent selectivity, anti-interference, a low detection limit and rapidity responsiveness to Al/Zn, coupled with a high fluorescence quantum yield and substantial Stokes shift (220 nm).