A facile and intelligent detection method for diclazuril based on a stable dual emissive Eu-dopped metal-organic framework.

Diclazuril (DIC) is a broad-spectrum anti-coccidiosis drug of the triazine class, widely used in poultry farming. The overuse of DIC may lead to its accumulation in animal bodies, which may enter the food chain and threaten human health. In this work, we fabricated a stable Eu-doped UiO-66 fluorescence sensor (EuUHIPA-30) for the sensitive detection of DIC.

Multifunctional Eu-MOF for simultaneous quantification of malachite green and leuco-malachite green and efficient adsorption of malachite green.

Multi-target detection combined with in-situ removal of contaminants is a challenging issue difficult to overcome. Herein, a dual-emissive Eu-metal organic framework (Eu-MOF) was constructed by pre-functionalization with a blue-emissive ligand and post-functionalization with red-emissive Eu ions using a UiO-66 precursor. The fluorescence of the synthesized Eu-MOF is highly selective and sensitive toward malachite green (MG) and its metabolite leuco-malachite green (LMG), which are environmentally persistent and highly toxic to humans.

A recyclable Eu-functionalized dual-emissive metal-organic framework for portable, rapid detection and efficient removal of malachite green.

A europium-functionalized, dual-emissive, metal-organic framework-based fluorescence sensor (EuUCNDA) was constructed post-synthetic modification of an UiO-66-type precursor through coordination interactions. EuUCNDA exhibited extremely high selectivity and sensitivity for malachite green (MG) with a low detection limit of 13.01 nM, a wide linear concentration range (0.

Nanozymatic Biofuel Cell-Enabled Self-Powered Sensing System for a Sensitive Immunoassay.

Self-powered sensing system (SPSS) integrating the enzymatic biofuel cell and biosensing platform has attracted tremendous interest. However, natural enzymes suffer from the intrinsic drawbacks of enzymes and enzymatic proteins. Nanozymes with enzyme-like activities are the ideal alternatives to enzymes, and it is greatly challenging to explore high-performance nanozymatic biofuel cell for SPSS.

Facile one-precursor and one-pot synthesis of Girard's reagent T-based carbon dots for bacteria-resistant and anti-biofilm applications.

Based on the promising development of carbon dots in antibacterial applications, Girard's reagent T-based carbon dots () with a mean size of 2.41 nm and excellent antibacterial performance were synthesized through a one-step method. The minimum inhibitory concentration ofwas 200 μg mlfor bothand.

PM2.5 exposure triggers cell death through lysosomal membrane permeabilization and leads to ferroptosis insensitivity via the autophagy dysfunction/p62-KEAP1-NRF2 activation in neuronal cells.

PM2.5 exposure can be associated with the onset of neurodegenerative diseases, with oxidative stress-induced cellular homeostasis disruption and cell death as one of the main mechanisms. However, the exact cellular and molecular processes are still rarely investigated.

A highly water-stable dual-emission fluorescent probe based on Eu-loaded MOF for the simultaneous detection and quantification of Fe and Al in swine wastewater.

A novel dual-emissive Eu-loaded metal-organic framework (MOF) is designed and successfully fabricated by introducing 2-hydroxyterephthalic acid (HBDC-OH) and Eu ions into an UiO-66-type MOF material. The obtained MOF, here referred to as EuUCH, exhibits dual emission at 450 and 614 nm, and both emissions are quite stable in aqueous media in the pH range of 4-11. EuUCH is characterized by a high selectivity and sensitivity toward Fe and Al, which yield different responsive modes.

A dual-channel "on-off-on" fluorescent probe for the detection and discrimination of Fe and Hg in piggery feed and swine wastewater.

Blue-fluorescent blood-CDs were synthesized through a one-pot hydrothermal method using a mixture of chicken blood and trisodium citrate and then explored as a fluorescent probe for detecting Fe and Hg. The probe showed excellent selectivity and sensitivity towards Fe and Hg with a dramatic "on-off" fluorescence response. F recovered the fluorescence quenching by Fe, and Al recovered the fluorescence quenching by Hg, showing an "off-on" fluorescence response.

A luminescent Eu-functionalized MOF for sensitive and rapid detection of tetracycline antibiotics in swine wastewater and pig kidney.

Tetracyclines (TCs), a type of antibiotics, are widely used in human therapy and animal husbandry. Public concerns about tetracyclines residues have been raised due to their negative impact on the environment and food, causing bacterias drug resistance and human health concerns. In this work, a luminescent europium MOF (EuUCBA) is constructed via post-synthetic attachment of Eu into a UiO-66 type MOF.

Recyclable europium functionalized metal-organic fluorescent probe for detection of tryptophan in biological fluids and food products.

An europium functionalized metal-organic fluorescent probe, Eu@UiO-66-FDC was constructed by post-synthetic modification through coordination interactions. Eu@UiO-66-FDC displayed high selectivity and sensitivity toward Tryptophan (Trp) among all the 20 natural amino acids and other general compounds in food and biological samples, with a wide linear concentration range (0-1000 μM), low detection limit (0.29 μM), and a rapid response (<1 min).

Highly sensitive and rapid detection of thiabendazole residues in oranges based on a luminescent Tb-functionalized MOF.

Thiabendazole (TBZ), has been extensively employed as a pesticide and/or a fungicide in agriculture, while its residues would threaten to public health and safety. Simple, rapid and sensitive probes for detection of TBZ in real food samples is significantly desirable. In present work, a highly selective and sensitive luminescent sensor for monitoring TBZ in oranges has been constructed based on a Tb-functionalized Zr-MOF (Tb@1).

A dual-analytes responsive fluorescent probe for discriminative detection of ClO and NH in living cells.

Hydrazine (NH) and ClO are very harmful for public health, hence it is important and necessary to monitor them in living cells. Herein, we rationally designed and synthesized a dual-analytes responsive fluorescent sensor PTMQ for distinguishing detection of NH and ClO. PTMQ underwent NH-induced double bond cleavage, affording colorimetric and green fluorescence enhancement with good selectivity and a low detection limit (89nM).

A distinctive mitochondrion-targeting, in situ-activatable near-infrared fluorescent probe for visualizing sulfur dioxide derivatives and their fluctuations in vivo.

Sulfur dioxide derivatives are intimately involved in some physiological processes in organisms, and high levels of these substances can cause many diseases. Herein, we rationally prepared a mitochondrion-targeting, in situ-activatable near-infrared (NIR) fluorescent probe (DCQN) by coupling 2-(3,5,5-trimethylcyclohex-2-enylidene)malononitrile with 3-quinolinium carboxaldehyde. DCQN displayed a NIR fluorescence turn-on signal to indicate the presence of HSO3-, along with a considerable hyperchromic shift from light yellow to purple via a 1,4-nucleophilic addition reaction.

Highly sensitive and rapid responsive fluorescence probe for determination of formaldehyde in seafood and in vivo imaging application.

Mutagenic formaldehyde (FA) is usually abused to preserve seafood, thus it is very necessary to detect harmful formaldehyde in seafood with a simple and effective method. In this work, we facilely prepared a new fluorescent probe RBNA, which showed a remarkable fluorescence lighting-up response towards FA with high sensitivity and selectivity, fast response (within 5 min) and a low detection limit (0.21 μM).

A selective cascade reaction-based probe for colorimetric and ratiometric fluorescence detection of benzoyl peroxide in food and living cells.

A novel colorimetric and ratiometric fluorescent probe (Cou-BPO) was readily prepared for specific detection of harmful benzoyl peroxide (BPO). The probe Cou-BPO reacted with BPO via a selective oxidation cleavage-induced cascade reaction of the pinacol phenylboronate group, which resulted in an observable colorimetric and ratiometric fluorescence response towards BPO with a fast response time (<15 min) and a low detection limit (56 nM). For practical application, facile, portable and sensitive test paper of Cou-BPO has been prepared for visual detection of BPO.

Usefulness of serum interleukin-33 as a prognostic marker of severe traumatic brain injury.

Background: Interleukin-33 is recently identified as a brain injury biomarker. We determined whether serum interlerukin-33 concentrations are associated with inflammation, severity and prognosis after traumatic brain injury (TBI).

Methods: We detected serum interlerukin-33 concentrations of 102 healthy controls and 102 severe TBI patients, as well as serum concentrations of 3 inflammatory biomarkers (interleukin-6, tumor necrosis factor-alpha and C-reactive protein) and 7 cell-specific proteins (myelin basic protein, glial fibrillary astrocyte protein, S100B, neuron-specific enolase, phosphorylated axonal neurofilament subunit H, Tau and ubiquitin carboxyl-terminal hydrolase L1) in 102 severe TBI patients.

High through-put determination of 28 veterinary antibiotic residues in swine wastewater by one-step dispersive solid phase extraction sample cleanup coupled with ultra-performance liquid chromatography-tandem mass spectrometry.

We developed a QuEChERS (quick, easy, cheap, effective, rugged and safe) method for the high through-put determination of 28 common veterinary antibiotics in swine wastewater using one-step dispersive solid-phase extraction (d-SPE) for sample cleanup and ultra-performance liquid chromatography-tandem mass spectrometry for detection. The orthogonal test method was used to systematically investigate the parameters that might influence d-SPE efficiency. The optimal d-SPE procedure utilized 40 mg primary secondary amine sorbent and 3 g L NaEDTA.

Branched Sialylated N-glycans Are Accumulated in Brain Synaptosomes and Interact with Siglec-H.

Proper N-glycosylation of proteins is important for normal brain development and nervous system function. Identification of the localization, carrier proteins and interacting partners of N-glycans is essential for understanding the roles of glycoproteins. The present study examined the N-glycan A2G'2F (Galβ1-3GlcNAcβ1-2Manα1-6[Galβ1-3GlcNAcβ1-2Manα1-3]Manβ1-4GlcNAcβ1-4[Fucα1-6]GlcNAc-).

A rhodamine-based fluorescent probe for colorimetric and fluorescence lighting-up determination of toxic thiophenols in environmental water and living cells.

Thiophenols are a class of highly toxic environmental pollutant, hence it is very necessary to monitor thiophenols in environment and living cells with an efficient and reliable method. Herein, a novel fluorescent probe for thiophenols has been developed, which exhibited a colorimetric and fluorescence turn-on dual response towards thiophenols with good selectivity and fast response. The sensing mechanism for thiophenols was attributed to nucleophilic substitution reaction, which was confirmed by HPLC.

Membrane IL1α Inhibits the Development of Hepatocellular Carcinoma via Promoting T- and NK-cell Activation.

Hepatocellular carcinoma is a worldwide health problem with limited treatment options and poor prognosis. Inflammation associated with liver injury and hepatocyte regeneration can lead to fibrosis, cirrhosis, and eventually, hepatocellular carcinoma. IL1α is one of the most important inflammatory cytokines involved in inflammation and tumor development.

Secreted IL-1α promotes T-cell activation and expansion of CD11b(+) Gr1(+) cells in carbon tetrachloride-induced liver injury in mice.

Interleukin-1α is mainly expressed on the cell membrane, but can also be secreted during inflammation. The roles of secreted and membrane IL-1α in acute liver inflammation are still not known. Here, we examined the functions of secreted and membrane IL-1α in a mouse model of carbon tetrachloride-induced acute liver injury.

Donor NK Cells and IL-15 promoted engraftment in nonmyeloablative allogeneic bone marrow transplantation.

Donor NK cells could promote engraftment by suppressing host alloreactive responses during allogeneic bone marrow transplantation (allo-BMT). The biological activity of NK cells could be significantly enhanced by IL-15. The current study attempted to evaluate the effect of donor NK cells and IL-15 administration on engraftment and immune reconstitution in a murine nonmyeloablative allo-BMT model.