In this study, polysaccharides from Angelica sinensis were extracted using the ultrasound-assisted extraction method. Based on the results of single factor experiments and orthogonal tests, three independent variables-water/raw material ratio, ultrasound time, and ultrasound power-were selected for investigation. Then, we used response surface methodology to optimize the extraction conditions.
View Article and Find Full Text PDFSpectrochim Acta A Mol Biomol Spectrosc
September 2017
Fluorescence quenching was used to study the potential interaction mechanism of Bovine serum albumin (BSA) with either hydrophilic ferroferric oxide (FeO) nanoparticles (NPs) or hydrophobic FeO NPs. The experimental results indicated the mechanism between BSA and hydrophilic FeO NPs was static quenching and the one between BSA and hydrophobic FeO NPs was dynamic process that was drove by Förster's resonance energy transfer (FRET). And the binding parameters for the interaction of BSA with either hydrophilic or hydrophobic FeO NPs were calculated by using the fluorescence quenching measurement.
View Article and Find Full Text PDFJ Chromatogr B Analyt Technol Biomed Life Sci
January 2017
This study investigated a new and easy-to-industrialized extracting method for curcumin from Curcuma longa rhizomes using ultrasonic extraction technology combined with ammonium sulfate/ethanol aqueous two-phase system (ATPS), and the preparation of curcumin using the semi-preparative HPLC. The single-factor experiments and response surface methodology (RSM) were utilized to determine the optimal material-solvent ratio, ultrasonic intensity (UI) and ultrasonic time. The optimum extraction conditions were finally determined to be material-solvent rate of 3.
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