Overproduction of Patchoulol in Metabolically Engineered .

Patchoulol, a plant-derived sesquiterpene compound, is widely used in perfumes, cosmetics, and pharmaceuticals. Microbial production provides a promising alternative approach for the efficient and sustainable production of patchoulol. However, there are no systematic engineering studies on aimed at achieving high-yield patchoulol production.

The fermentation of compound oral liquid by human colonic microbiota altered the abundance of probiotics and short-chain fatty acid production.

Compound oral liquid (COL), made from functional herbal foods, has gained immense popularity in China for healthcare. However, the interaction between the nutrients in COL and gut microbiota is still unclear. In our study, the content of total flavonoids, polyphenols, and proteins was increased and the total sugar reduced by crushing raw ingredients to 10 mesh (COL-C).

Association of human gut microbiota composition and metabolic functions with Ficus hirta Vahl dietary supplementation.

Ficus hirta Vahl (FHV), a traditional herbal ingredient of the tonic diet, receives increasing popularity in southern China. However, it is largely unknown that how a FHV diet (FHVD) affects the human gut microbiome. In this exploratory study, a total of 43 healthy individuals were randomized into the FHVD (n = 25) and Control (n = 18) groups to receive diet intervention for 8 weeks.

Modulation of Gut Microbiota Composition and Short-Chain Fatty Acid Synthesis by Mogroside V in an Incubation System.

Mogroside V (MV), a sweetener, is one of the major components in. In our research, after incubation with MV for 24 h, the human gut microbiota diversity changed, with an enrichment of the genera , , , , and and the inhibition of XlVa, , and . Moreover, the synthesis of short-chain fatty acids, such as acetate, propionate, and butyrate, was increased by gut microbiota.

Application of RecET-Cre/loxP system in Corynebacterium glutamicum ATCC14067 for L-leucine production.

Objective: To explore the RecET-Cre/loxP system for chromosomal replacement of promoter and its application on enhancement L-leucine production in Corynebacterium glutamicum (C. glutamicum) ATCC14067.

Results:  The RecET-Cre/loxP system was used to achieve the chromosomal replacement of promoter in C.

Multiplex gene editing and large DNA fragment deletion by the CRISPR/Cpf1-RecE/T system in Corynebacterium glutamicum.

Corynebacterium glutamicum is an essential industrial strain that has been widely harnessed for the production of all kinds of value-added products. Efficient multiplex gene editing and large DNA fragment deletion are essential strategies for industrial biotechnological research. Cpf1 is a robust and simple genome editing tool for simultaneous editing of multiplex genes.

Synthetic biology approaches to access renewable carbon source utilization in Corynebacterium glutamicum.

Corynebacterium glutamicum (C. glutamicum), an important industrial workhorse, is capable of efficiently producing a variety of value-added chemicals and fuels beyond amino acids. C.