PdeERF114 recruits PdeWRKY75 to regulate callus formation in poplar by modulating the accumulation of H O and the relaxation of cell walls.

Callus formation is important for numerous biological processes in plants. Previously, we revealed that the PdeWRKY75-PdeRBOHB module positively regulates hydrogen peroxide (H O ) accumulation, thereby affecting callus formation in poplar. In this study, we identified and confirmed a transcription factor, PdeERF114, that interacts with PdeWRKY75 both in vitro and in vivo.

Uncovering the Role of PdePrx12 Peroxidase in Enhancing Disease Resistance in Poplar Trees.

Peroxidase (Prx)-related genes are reported to be involved in the metabolism of hydrogen peroxide (HO) in plants. Here, we found that the expression of the gene was upregulated in wild-type (WT) poplar line NL895 infected with the pathogens strain 3C and strain 3E. The gene was cloned in the poplar line NL895 and its overexpression (OE) and reduced-expression (RE) vectors were constructed.

Experimental Study on Carbonation of Cement-Based Materials in Underground Engineering.

The corrosive water environment has a decisive influence on the durability of a diversion tunnel lining. In this paper, the effects of carbonation on cement-based materials in water-immersion and saturated-humidity environments were studied by increasing the CO concentration. The results show that under conditions of water-immersion and saturated humidity, the color of the non-carbonation region is dark, while the carbonation region is gray, and the color boundary is obvious.

The chromosome-scale genome sequence of Triadica sebifera provides insight into fatty acids and anthocyanin biosynthesis.

The Chinese tallow tree (Triadica sebifera) can produce oil with high content of unsaturated fatty acids in seeds and shows attractive leaf color in autumn and winter. Here, the 739 Mb chromosome-scale genome sequence of the Chinese tallow tree was assembled and it reveals the Chinese tallow tree is a tetraploid. Numerous genes related to nutrition assimilation, energy utilization, biosynthesis of secondary metabolites and resistance significantly expanded or are specific to the Chinese tallow tree.

Phosphate (Pi) stress-responsive transcription factors PdeWRKY6 and PdeWRKY65 regulate the expression of PdePHT1;9 to modulate tissue Pi concentration in poplar.

Phosphorus (P) is an important nutrient for plants. Here, we identify a WRKY transcription factor (TF) in poplar (Populus deltoides × Populus euramericana) (PdeWRKY65) that modulates tissue phosphate (Pi) concentrations in poplar. PdeWRKY65 overexpression (OE) transgenic lines showed reduced shoot Pi concentrations under both low and normal Pi availabilities, while PdeWRKY65 reduced expression (RE) lines showed the opposite phenotype.

Investigation on Water Transformation and Pore Structure of Cement-Stabilized Dredged Sediment Based on NMR Technology.

Cement-stabilized dredged sediment (CDS) when used as a new road construction material cannot only solve the problem of abandoned sediment disposal, but also effectively save natural soil resources. This study aimed to evaluate the strength and permeability of CDS and establish corresponding prediction models from the perspective of a stabilization mechanism. The soil-water composition and pore size distribution were investigated by the nuclear magnetic resonance (NMR) technique.

The transcription factor WRKY75 regulates the development of adventitious roots, lateral buds and callus by modulating hydrogen peroxide content in poplar.

Hydrogen peroxide (H2O2) plays important roles in plant development. Adventitious roots (AR), lateral buds (LB) and callus formation are important traits for plants. Here, a gene encoding RESPIRATORY BURST OXIDASE HOMOLOG B (PdeRBOHB) from poplar line 'NL895' (Populus.

Sewage sludge ash-incorporated stabilisation/solidification for recycling and remediation of marine sediments.

Finding suitable disposal sites for dredged marine sediments and incinerated sewage sludge ash (ISSA) is a challenge. Stabilisation/solidification (S/S) has become an increasingly popular remediation technology. This study sheds light on the possible beneficial use of ISSA together with traditional binders to stabilise/solidify marine sediments.

Gain-of-function of the 1-aminocyclopropane-1-carboxylate synthase gene ACS1G induces female flower development in cucumber gynoecy.

Unisexual flowers provide a useful system for studying plant sex determination. In cucumber (Cucumis sativus L.), three major Mendelian loci control unisexual flower development, Female (F), androecious [a; 1-aminocyclopropane-1-carboxylate {ACC} synthase 11, acs11], and Monoecious (M; ACS2), referred to here as the Female, Androecious, Monoecious (FAM) model, in combination with two genes, gynoecious (g, the WIP family C2H2 zinc finger transcription factor gene WIP1) and the ethylene biosynthetic gene ACC oxidase 2 (ACO2).

Isolation, identification and bioactivity of endophytic fungi from medicinal plant Malus sieboldii.

Objective: To isolate and identify endophytic fungi from Malus sieboldii, and detect cytotoxicity, protease inhibition and antifungal activities of their crude extracts.

Method: The fungi were identified with the aid of morphology or Internal Transcribed Spacer (ITS) rDNA molecular methods. Fungal activities were tested by cylinder-plate, MTT and BRpNA methods, respectively.