Metabolite Profiling Analysis of the Tongmai Sini Decoction in Rats after Oral Administration through UHPLC-Q-Exactive-MS/MS.

Tongmai Sini decoction (TSD), the classical prescriptions of traditional Chinese medicine, consisting of three commonly used herbal medicines, has been widely applied for the treatment of myocardial infarction and heart failure. However, the absorbed components and their metabolism in vivo of TSD still remain unknown. In this study, a reliable and effective method using ultra-performance liquid chromatography coupled with hybrid quadrupole-Orbitrap mass spectrometry (UHPLC-Q-Exactive-MS/MS) was employed to identify prototype components and metabolites in vivo (rat plasma and urine).

[Study on modern processing technologies of aconiti kusnezoffii radix].

Objective: To compare different processing technologies of Aconiti Kusnezoffii Radix, and to optimize a kind of "low toxicity and high efficiency", simple and practical processing technology for Aconiti Kusnezoffii Radix.

Methods: HPLC method was used to determine the content of six kinds of alkaloids, and titration method to determine the content of total alkaloids. These contents combined with production were used to evaluate the modern processing technologies of Aconiti Kusnezoffii Radix.