How biofilm and granular sludge cope with dissolved oxygen exposure in anammox process: Performance, bioaccumulation characteristics and bacterial evolution.

In order to study the resistance mechanisms of biofilm and granular sludge to various dissolved oxygen (DO) exposures in anaerobic ammonium oxidation (anammox) process, a biofilm - granular sludge anammox reactor was established and operated. Experimental results showed that DO levels of ≤0.41 mg L hardly affected the total nitrogen removal efficiency (TNRE).

The role of hydroponic solution of Ulva fasciata in modulating the antibiotic resistance of Vibrio alginolyticus.

Aims: This study aimed to ascertain the active substances in the hydroponic solution of Ulva (U.) fasciata (HSUF) and their effects on antibiotics resistance of pathogenic bacteria Vibrio alginolyticus (Va.) at environmental levels.

Performance and microbial mechanism in sulfide-driven autotrophic denitrification by different inoculation sources in face of various sulfide and sulfate stress.

To develop a reliable sulfide (S) autotrophic denitrification (SAD) process under S and SO salinity stresses, the biofilm performance and microbial mechanisms were comparatively studied using different inocula of activated sludge (AS) and intertidal sediment (IS). Biofilm IS enriched more denitrification genes (0.34 %) and S oxidation genes (0.

Response of anammox to organics with different degradation characteristics and exposure time: Performance, sludge characteristics and bacterial community.

The effects of typical organic compounds including easily degradable organic matters sodium acetate, yeast and methanol, and refractory organic matter (ROM) humic acid on anaerobic ammonium oxidation (anammox) systems in short-term and medium-term exposure time were studied. During short-term experiments, nitrogen removal activity (NRA) was inhibited at sodium acetate level of 150 mg L total organic carbon (TOC) and methanol level of 30-150 mg L TOC, but humic acid and yeast (≤150 mg L TOC) enhanced nitrogen removal in anammox systems. The greatest NRA of 30.

Performance, kinetic characteristics and bacterial community of short-cut nitrification and denitrification system at different ferrous ion conditions.

In order to explore the operation performance, kinetic characteristics and bacterial community of the short-cut nitrification and denitrification (SND) system, the SND system with pre-cultured short cut nitrification and denitrification sludge was established and operated under different ferrous ion (Fe (II)) conditions. Experimental results showed that the average NH-N removal efficiency (ARE) of SND system was 97.3% on Day 5 and maintained a high level of 94.

A collaborative effect of solid-phase denitrification and algae on secondary effluent purification.

This study explored the collaborative effect on nutrients removal performance and microbial community in solid-phase denitrification based bacteria-algae symbiosis system. Three biodegradable carriers (apple wood, poplar wood and corncob) and two algae species (Chlorella vulgaris and Chlorella pyrenoidosa) were selected in these bacteria-algae symbiosis systems. Results demonstrated that corncob as the carrier exhibited the highest average removal efficiencies of total nitrogen (83.

Simultaneous denitrification and organics removal by denitrifying bacteria inoculum in a multistage biofilm process for treating desulfuration and denitration wastewater.

This study aimed to treat real wastewater from the desulfuration and denitration process in a petrochemical plant with high-strength nitrogen (TN≈200 mg/L, > 90% nitrate), sulfate (2.7%) and extremely low-strength organics (COD < 30 mg/L). Heterotrophic denitrification of multistage anoxic and oxic biofilm (MAOB) process in three tanks using facultative denitrifying bacteria inoculum was developed to simultaneously achieve desirable effluent nitrogen and organics at different hydraulic retention time (HRT) and carbon to nitrogen (C/N) mass ratios.

Improving denitrification performance of biofilm technology with salt-tolerant denitrifying bacteria agent for treating high-strength nitrate and sulfate wastewater from lab-scale to pilot-scale.

This study focused on the application of salt-tolerant denitrifying bacteria (DBA) in an optimized biofilm process to treat high sulfate-nitrate wastewater from lab-scale to pilot-scale. Lab-scale results demonstrated the salinity, DBA inoculum, supplementary carbon and phosphorus source significantly varied the startup periods at the range of 36-74 d, and the optimum initial start-up conditions were as follows: >0.6 g/L of DBA, 2-4 of C/N ratio, 0.

Enhanced removal of mixed VOCs with different hydrophobicities by Tween 20 in a biotrickling filter: Kinetic analysis and biofilm characteristics.

Mass transfer limitation usually causes the poor performance of biotrickling filters (BTFs) for the treatment of hydrophobic volatile organic compounds (VOCs) during long-term operation. In this study, two identical lab-scale BTFs were established to remove a mixture of n-hexane and dichloromethane (DCM) gases using non-ionic surfactant Tween 20 by Pseudomonas mendocina NX-1 and Methylobacterium rhodesianum H13. A low pressure drop (≤110 Pa) and a rapid biomass accumulation (17.

Characteristics of exopolysaccharides produced by isolates from natural bioflocculant of conglutination mud.

Three novel types of exopolysaccharides (EPS) EPS-S8, EPS-S5, and EPS-F10 were extracted and purified from bacterial isolates sp. GHS8, sp. GHS5 and sp.

Antibiotics resistance evolution of isolated Vibrio parahaemolyticus from mariculture under the continuous culture of sub-inhibitory concentrations of Ulva fasciata hydroponic solution.

The outbreak of vibriosis from Vibrio (V.) parahaemolyticus is widespread in the mariculture, and live macroalgae has been considered to be effective and eco-friendly approach for the control of vibriosis. Three V.

Interaction of tetrahydrofuran and methyl tert-butyl ether in waste gas treatment by a biotrickling filter bioaugmented with Piscinibacter caeni MQ-18 and Pseudomonas oleovorans DT4.

Bioaugmented biotrickling filter (BTF) seeded with Piscinibacter caeni MQ-18, Pseudomonas oleovorans DT4, and activated sludge was established to investigate the treatment performance and biodegradation kinetics of the gaseous mixtures of tetrahydrofuran (THF) and methyl tert-butyl ether (MTBE). Experimental results showed an enhanced startup performance with a startup period of 9 d in bioaugmented BTF (25 d in control BTF seeded with activated sludge). The interaction parameter I of control (7.

Efficient anaerobic bioremediation of high-concentration benzo[a]pyrene in marine environments.

Benzo[a]pyrene (BaP), a persistent organic pollutant that may accumulate in sea sediments after oil spill or BaP chemical leakage accidents, considerably harms marine ecosystems and human health. Previous studies have been predominantly focused on its degradation at low concentrations, while the remediation of BaP pollution with high concentrations was neglected. Additionally, the metabolic pathways associated with its anaerobic degradation remain unclear.

Anaerobic degradation of high-concentration polycyclic aromatic hydrocarbons (PAHs) in seawater sediments.

Recurring oil spill accidents have been a global challenge and contribute to PAHs' heavy accumulation in marine sediments. The rapid bioremediation of PAHs with high concentrations in marine sediments has rarely been studied. In this study, four representative PAHs in crude oil were tested for fast anaerobic biodegradation.

The role of Ulva fasciata in the evolution of the microbial community and antibiotic resistance genes in maricultural sediments.

This study explored changes in the microbial community and antibiotic resistance genes (ARGs) in maricultural clam sediment after 3-month co-culture with different densities (0, 5 and 12 g L) of seaweed Ulva fasciata (U. fasciata). The maximum removal rates of NO-N, PO-P, and inhibition of Vibrio culturability occurred at presence of 12 g LU.

Dynamics of denitrification performance and denitrifying community under high-dose acute oxytetracycline exposure and various biorecovery strategies in polycaprolactone-supported solid-phase denitrification.

Solid-phase denitrification (SPD) is a promising technology for nitrate-rich water purification. This study aimed to examine the variation in denitrification performance and denitrifying community under high-dose acute oxytetracycline (OTC) exposure and various biorecovery strategies. The denitrification performance was impaired significantly after one-day OTC shock at 50 mg L in a continuous-flow SPD system supported by a polycaprolactone (PCL) carrier but could rapidly recover without the addition of OTC.

Comparative analysis of denitrification performance, denitrifying community and functional genes to oxytetracycline exposure between single and hybrid biodegradable polymers supported solid-phase denitrification systems.

Biodegradable carrier are vital for the solid-phase denitrification (SPD) systems for treating nitrate-rich water. Two solid-phase denitrification reactors were developed with both 200 g L of single (polycaprolactone, PCL) (R1) and hybrid solid carbon sources (PCL/polylactic acid (PLA) /polyhydroxyalkanoates (PHA)) (R2) to examine the denitrification performance, denitrifying community and functional genes to various oxytetracycline (OTC) exposure in this study, respectively. Complete denitrification performance was achieved in the both SPD systems at low stress of OTC (1 mg L), but then dramatically reduced to less than 20% of nitrate reduction efficiency after one-month high OTC stress (10 mg L), and rapidly recovered to stable nitrate removal rates of 76.

Variation in microbial populations and antibiotic resistance genes in mariculture sediments in the present of the seaweed Ulva fasciata and under selective pressure of oxytetracycline.

The widely distributed seaweed Ulva fasciata has nutrient absorption abilities and can be used in the bioremediation of polluted maricultural environments. This study explored microbial community and antibiotic resistance gene (ARG) variation in mariculture sediments in response to different trace levels (10, 100, and 500 μg L) of oxytetracycline (OTC) and the presence of Ulva fasciata. The increase in OTC level promoted nutrient (NO_-N and PO-P) removal mainly due to Ulva fasciata adsorption.

Response of denitrifying community, denitrification genes and antibiotic resistance genes to oxytetracycline stress in polycaprolactone supported solid-phase denitrification reactor.

The coexistence of nitrate and antibiotics in wastewater is a common problem. The study aimed to explore the response of denitrifying community, denitrification genes and antibiotic resistance genes (ARGs) to oxytetracycline (OTC) stress in polycaprolactone (PCL) supported solid-phase denitrification (SPD) reactors. Complete nitrate reduction (greater than99%) was achieved in SPD system with OTC stress of 0, 0.

sp. nov., isolated from the toxigenic marine dinoflagellate LZT09.

A Gram-stain-negative, strictly aerobic, non-motile and non-pigmented spirillum, designated strain LZ-5, was isolated from cultures of the paralytic shellfish poisoning (PSP) toxin-producing marine dinoflagellate LZT09 collected from the Zhoushan sea area in the East China Sea during an algal bloom. The isolate grew at 4-40 °C (optimum, 25-33 °C) and pH 5.0-9.

Microbial origin of bioflocculation components within a promising natural bioflocculant resource of Ruditapes philippinarum conglutination mud from an aquaculture farm in Zhoushan, China.

Ruditapes philippinarum conglutination mud (RPM) is a byproduct from the aquiculture of an important commercially bivalve mollusk R. philippinarum and has been recently reported as a promising natural bioflocculant resource. However the origin of bioflocculation components within RPM is still a pending doubt and impedes its effective exploitation.

Assessment of nutrient removal and microbial population dynamics in a non-aerated vertical baffled flow constructed wetland for contaminated water treatment with composite biochar addition.

A novel composite biochar (NCB) was produced from the pyrolysis of co-fermentation products of sewage sludge, food wastes and rice straw, and exhibited higher nitrogen and phosphorus adsorption capacity due to the larger surface area (14.7 m g) and higher Ca content (51753.7 mg kg) than single rice straw biochar.