Comprehensive untargeted lipidomics study of black morel (Morchella sextelata) at different growth stages.

The black morel (Morchella sextelata) is a valuable edible and medicinal mushroom appreciated worldwide. Here, lipidomic profiles and lipid dynamic changes during the growth of M. sexletata were analyzed using ultra-performance liquid chromatography coupled with mass spectrometry.

Effect of vanadium and tungsten loading order on the denitration performance of F-doped VO-WO/TiO catalysts.

F-doped VO-WO/TiO catalyst has been confirmed to have excellent denitration activity at low temperatures. Since the VO-WO/TiO catalyst is a structure-sensitive catalyst, the loading order of VO and WO may affect its denitration performance. In this paper, a series of F-doped VO-WO/TiO catalysts with different VO and WO loading orders were synthesized to investigate the effect of denitration performance at low temperatures.

Secondary Metabolites from and Their Anti-Inflammatory Activities.

, a culinary and medicinal mushroom, is widely consumed in Asian countries. Chemical investigation on the fruiting bodies of led to the isolation of one new ergostane-type sterol fatty acid ester, erinarol K (); and eleven known compounds: 5α,8α -epidioxyergosta-6,22-dien-3β-yl linoleate (); ethyl linoleate (); linoleic acid (); hericene A (); hericene D (); hericene E (); ergosta-4,6,8(14),22-tetraen-3-one (); hericenone F (); ergosterol (); ergosterol peroxide (); 3β,5α,6α,22-ergosta-7,22-diene-3,5,6-triol 6-oleate (). The chemical structures of the compounds were determined by 1D and 2D NMR (nuclear magnetic resonance) spectroscopy, mass spectra, etc.

Integration of Transcriptomics and Metabolomics for Understanding the Different Vegetative Growth in .

is an edible and medicinal fungus with high nutritional, medicinal, and economic value. Recently, . has been produced through artificial cultivation in China, but its stable production remains problematic because the details of its growth and development process are limitedly understood.

Untargeted Metabolomics Reveals Alterations in the Primary Metabolites and Potential Pathways in the Vegetative Growth of Morchella sextelata.

one of the true morels, has recently been artificially cultivated with stable production in China. Analysis of the variations in primary metabolites during the vegetative stages of is critical for understanding the metabolic process. In this study, three developmental stages were categorized based on morphological and developmental changes, including the young mushroom period, marketable mature period, and physiological maturity period.

Chemical Composition of Aromas and Lipophilic Extracts from Black Morel () Grown in China.

Morels ( spp.) are valuable medicinal and edible mushrooms. In this study, chemical profiles of aromas and lipophilic extracts of black morel () grown in China were analyzed by gas chromatography/mass spectroscopy, along with the evaluation of antioxidant and antimicrobial activities for the lipophilic extracts.

Chemical Composition and Antimicrobial Activity of Essential Oil from Collected in Ethiopia.

The essential oil from , a traditional herb of Ethiopia, has been studied, including the chemical composition and antimicrobial activity. The difference between four samples (P-1⁻P-4), which differed in gender or location, has also been analyzed. The essential oils were obtained by steam distillation, while the aromas were extracted by head space solid-phase microextraction (HS-SPME) and both were analyzed by gas chromatography- mass spectrometry (GC-MS).

Biochemical and Structural Characterization of TtnD, a Prenylated FMN-Dependent Decarboxylase from the Tautomycetin Biosynthetic Pathway.

Tautomycetin (TTN) is a polyketide natural product featuring a terminal alkene. Functional characterization of the genes within the ttn gene cluster from Streptomyces griseochromogenes established the biosynthesis of the TTN polyketide backbone, its dialkylmaleic anhydride moiety, the coupling of the two moieties to form the nascent intermediate TTN F-1, and the tailoring steps converting TTN F-1 to TTN. Here, we report biochemical and structural characterization of TtnD, a prenylated FMN (prFMN)-dependent decarboxylase belonging to the UbiD family that catalyzes the penultimate step of TTN biosynthesis.

Cytotoxic sesquiterpenoids from Ligularia pleurocaulis.

The phytochemical investigation of whole plants of Ligularia pleurocaulis led to isolation of five sesquiterpenoids, including an eremophilenolide dimer named biligupleurolide, along with eight known sesquiterpenoids. Their structures were determined by spectroscopic methods including 2D NMR techniques, and the structures of biligupleurolide and ligupleurolide were confirmed by X-ray diffraction analysis. All of the isolated compounds were tested for their cytotoxic activity against three cancer cell lines (HepG2, A549 and MCF-7), and biligupleurolide showed moderate inhibitory activities, with IC50 values of 5.

Terpenoids from Ligularia kangtingensis.

Background: Ligularia kangtingensis, a species from the genus Ligularia (Compositae), is an indigenous plant in Southwest China and more than 20 species in this genus have been used as folk medicines in China.

Objective: The chemical constituents of the whole plant of L. kangtingensis were studied.

Strain prioritization for natural product discovery by a high-throughput real-time PCR method.

Natural products offer unmatched chemical and structural diversity compared to other small-molecule libraries, but traditional natural product discovery programs are not sustainable, demanding too much time, effort, and resources. Here we report a strain prioritization method for natural product discovery. Central to the method is the application of real-time PCR, targeting genes characteristic to the biosynthetic machinery of natural products with distinct scaffolds in a high-throughput format.

Structural and stereochemical studies of a tetralin norsesquiterpenoid from Ligularia kangtingensis.

A chiral tetralin norsesquiterpenoid, ligukangtinol, was isolated from the plant Ligularia kangtingensis. Its planar structure was determined by extensive analysis of spectroscopic data (MS, IR, and NMR), and (1S,3R) absolute configuration of the tetralin ring was established by TDDFT-ECD calculations of the solution conformers. Conformational analysis and ECD calculations proved that the semiempirical helicity rules of 6-hydroxytetralins correlating the (1)L(b) Cotton effect and P/M helicity of the fused carbocyclic ring correctly predicts the absolute configuration and thus can be used for the configurational assignment of related substituted tetralin derivatives.

Sibiralactone: a new monoterpene from Sibiraea angustata.

Sibiralactone (1), a new monoterpene derivative, was isolated from the leaves of Sibiraea angustata. The structure was determined by the analysis of its NMR data and the absolute configuration was established by TDDFT ECD calculation of the solution conformers.

Triterpenoid glycosides from the leaves of Ilex pernyi.

Ten triterpenoid glycosides, including five new ones (1-5), were isolated from the leaves of Ilex pernyi. The chemical structures of 1-5 were determined on the basis of the chemical and spectroscopic evidence.

An effective O-demethylation of some C19-diterpenoid alkaloids with HBr-glacial acetic acid.

The aconitine-type alkaloids talatisamine (1), 8,14-diacetyltalatisamine (11), and compound 3, the lycoctonine-type alkaloid deltaline (5), and the 7,17-seco C(19)-diterpenoid alkaloids 7 and 9 were treated with HBr-glacial acetic acid to give useful O-demethylated derivatives 2, 2, 4, 6, 8, and 10 respectively in good to high yields (49-90%).

Chemical constituents from the leaves of Ilex pernyi.

A new compound and five known compounds were isolated from the ethanolic extract of the leaves of Ilex pernyi Franch. Their structures were established on the basis of spectral analysis and identified as trans-isoeugenyl-alpha-L-arabinopynosyl (1 --> 6) -beta-D-glucopyranoside (1) , kaempferol-3-O-sambubioside (2), quercetin-3-O-sambubioside (3), isoquercitrin (4), (+) -syringaresinol-O-beta-D-glucopyranoside (5), amarantholidoside IV (6). Among them, compound 1 is a new phenolic glycoside, named as ilexperphenoside A, and compounds 2-6 were isolated from this plant for the first time.

[Studies on triterpenoid constituents in leaf of Ilex pernyi].

Objective: To investigate the chemical constituents of Ilex pernyi.

Method: The chemical constituents were isolated by various column chromatographic methods. The structures were identified by spectral data.

Complete assignments of (1)H and (13)C NMR spectroscopic data for two new triterpenoid saponins from Ilex pernyi.

Ilexpernoside A and ilexpernoside B, two new pentacyclic C(4)-nortriterpenoid saponins, were isolated from the leaves of Ilex pernyi Franch. Their chemical structures were determined by MS, NMR spectroscopy and chemical analysis. Complete assignments of the (1)H and (13)C NMR spectroscopic data were achieved by 1D and 2D NMR experiments (HSQC, HMBC, (1)H-(1)H COSY and NOESY).